Article Figures & Data
Figures
- FIG. 1.
Correlation between HBV DNA determinations within dynamic ranges using the Abbott RealTime and Versant assays for samples from 59 HBV-infected, HBeAg-negative patients for which a quantitative result was obtained with both assays. The identity line (solid line) as well as the best-fit correlation (broken line) is shown (y = 0.9863x − 0.154, R2 = 0.8646).
- FIG. 2.
Correlation between HBV DNA determinations within dynamic ranges using the RealTime and Versant assays for samples from 59 HBV-infected, HBeAg-negative patients according to surface gene variants (genotypes are indicated).
- FIG. 3.
Correlation between HBV DNA determinations within dynamic ranges using the RealTime and Versant assays for samples from 59 HBV-infected, HBeAg-negative patients according to the number of polymerase gene mutations.
- FIG. 4.
Correlation between HBV DNA determinations using the RealTime and other quantitative PCR assays in HBV DNA samples isolated from patients in cohort B for which a quantitative result was obtained with both assays. (Top) CAM assay (n = 66). (Bottom) CAP-CTM assay (n = 101). The best-fit correlation (solid line) is shown (for the CAM assay, y = 0.8653x + 0.3629 and R2 = 0.7668; for the CAP-CTM assay, y = 0.9586x − 0.0543 and R2 = 0.9276).
Tables
- TABLE 1.
Genotypic characterization of HBeAg-negative HBV strains isolated from 93 samples obtained from cohort A
HBV strain and relevant genotype No. of samples with genotype Surface gene variants A 31 A/G 2 B 9 C 7 D 35 E 6 F 2 G 1 Polymerase gene variants Wild-type 34 L180M 1 M204I 14 M204V 1 L180M + M204I 3 L180M + M204V 39 L180M + M204I + M204V 1 - TABLE 2.
Comparison of the dynamic ranges of three HBV DNA quantification assaysa
Test and result No. of samples with the following result by Abbott RealTime assay: Below detection threshold Quantifiable Above quantification limit Versant assay (n = 93) Below detection threshold 1 3 0 Quantifiable 0 59 0 Above quantification limit 0 30 0 Amplicor assay (n = 79) Below detection threshold 0 0 0 Quantifiable 0 66 0 Above quantification limit 0 13 0 TaqMan assay (n = 106) Below detection threshold 0 2 0 Quantifiable 0 101 0 Above quantification limit 0 3 0 ↵a The dynamic range of the Abbott RealTime assay was compared with those of the Versant, Amplicor, and TaqMan assays for detecting HBV DNA in 93 samples isolated from cohort A (Versant) and 106 samples isolated from cohort B (TaqMan). The cohort tested with Amplicor is a subgroup of 79 samples from cohort B.
