DOI: 10.1128/JCM.01692-07
Article Figures & Data
Tables
- TABLE 1.
Primers and probes used in our singleplex real-time PCR assays for the detection of HHV-6 and GFP
Primer or probe Sequence HHV-6 forward primer 5′ AAAATTTCTCACGCCGGTATTC 3′ HHV-6 reverse primer 5′ CCTGCAGACCGTTCGTCAA 3′ HHV-6 probe 6-FAM-TCGGTCGACTGCCCGCTACCA-TAMRA GFP forward primer 5′ CACCCTCTCCACTGACAGAAAAT 3′ GFP reverse primer 5′ TTTCACTGGAGTTGTCCCAATTC 3′ GFP probe 6-FAM-TGTGCCCATTAACATCACCATCTAATTCAACA-TAMRA - TABLE 2.
Demographic, clinical, and laboratory information for the 24 HHV-6-positive patientsa
Patient no. Sample type Patient Days between onset and collection Diagnosis Symptom(s) CSF information HHV-6 real-time PCR CT value HHV-6 typing result Typing primer used Other agents detected Sex Age (yr) CSF abnormality Protein level (mg/dl) WBC count % Lymphs 1 CSF M 12 10 Encephalitis Fever, altered mental status, hallucinations Yes 56 35 95 31.78 B U86 2 CSF F 12 2 Encephalitis Fever, altered mental status No 33 4 Unknown 31.98 B U86 3 CSF F 2 7 Encephalitis Fever, altered mental status, seizures, muscle weakness Unknown Unknown Unknown Unknown 36.07 Untyped U86 4 CSF M 45 Unknown Encephalitis Fever, headache, seizures, altered mental status, muscle weakness, muscle pain Yes Unknown 20 80 34.3 Untyped U86 5 CSF M 2 1 Encephalitis Fever, seizures, altered mental status No 16 0 Unknown 39.3 B U95 6 CSF F 1 0 Unknown Fever, rash, altered mental status, increased AST/ALT Yes 483 2 50 38.65 Untyped U86 7 CSF M 2 0 Meningitis Fever, altered mental status, cough Unknown 28 Unknown Unknown 36.5 B U86 8 CSF F 30 31 Encephalitis; acute onset psychosis Headache, rash, altered mental status, confusion, hyperactivity Yes 13 10 100 32.29 B U95 9 CSF M 8 Unknown Encephalitis Seizures, altered mental status, muscle weakness, stiff neck Yes 35 58 90 37.58 B U95 10 CSF M 38 14 Encephalitis Fever, altered mental status, headache, stiff neck Yes Unknown Unknown Unknown 39.74 Untyped U95 EBV+ve 11 CSF F 1 Unknown Encephalitis Seizures Unknown Unknown Unknown Unknown 33.3 B U95 12 CSF/autopsy M 2 Unknown Encephalitis; meningitis Seizures Unknown Unknown Unknown Unknown 38.47 B U95 13 CSF M 17 1 Meningitis Headache, stiff neck, muscle weakness, muscle pain Yes 150 40 29 25.03 B U95 14 CSF F 81 5 Encephalitis Fever, headache, rash, altered mental status Unknown Unknown Unknown Unknown 33.56 Untyped U95 15 CSF F 4 0 Encephalitis Fever, seizures, altered mental status No 25 2 0 34.75 B U95 16 CSF F 8 3 Meningitis Fever, headache, stiff neck, meningeal sign Yes 66 228 58 39.92 B U95 17 Liver tissue F 30 7 Unknown Fever, rash, elevated LFT result, jaundice, pruritis N/A N/A N/A N/A 34 B U95 18 CSF M 60 1 Meningoencephalitis Fever, seizures, altered mental status Yes 49 99 N/A 28.89 B U95 19 CSF M 25 1 Meningitis Muscle weakness Yes 119 49 100 28.68 B U95 20 CSF/autopsy F 1 Unknown Unknown Sickle cell disease, anemia, fever Unknown Unknown Unknown Unknown 37.96 B U95 Adv+ve 21 CSF/autopsy M 2 Unknown Unknown Fever, cough, bronchiolitis symptoms Unknown Unknown Unknown Unknown 37.35 B U95 22 CSF M 71 8 Encephalitis Fever, altered mental status Yes 117 47 100 29.62 Untyped U95 EBV+ve 23A CSF F 3 16 Encephalitis; cerebellitis Muscle weakness, ataxia Unknown Unknown Unknown Unknown 32.2 B U95 23B Serum 27 Unknown N/A N/A N/A N/A 30.7 B U95 23C CSF 34 Headache, altered mental status, vomiting, tremor No 15 1 Unknown 36.09 B U95 24 CSF M 1 2 Encephalitis Fever, muscle weakness, altered mental status, seizures, rash Yes 288 5 65 32.9 B U95 ↵a F, female; M, male; AST/ALT, alanine aminotransferase/aspartate aminotransferase; LFT, liver function test; % Lymphs, number of lymphocytes as a percentage of total WBC count; N/A, not applicable; Adv, adenovirus. U86 and U95 are the genes targeted in the HHV-6 PCR typing assays. Three specimens were tested for patient 23 (A, B, and C).
