DOI: 10.1128/JCM.01997-06
Article Figures & Data
Figures
- FIG. 1.
Representative electrophoresis patterns of gyrA quinolone resistance-determining region (QRDR) PCR products and allele-specific PCR products amplified with mixed primers for determination of C261A, C261G, G271A, G271T and A272G mutant gyrA genotypes of H. pylori. The theoretical fragment sizes according to the gyrA sequence are mentioned in the text.
Tables
- TABLE 1.
Oligonucleotide sequences of primers used in AS-PCR method to determine gyrA mutation of H. pylori at positions 261, 271, and 272
Primer direction Mutation Primer name Primer sequencea PCR product size (bp) Forward C261A F261A1 CCCCCATGGCGAGAAaG 262 C261G F261G1 CCCCCATGGCGAGAAgG 262 G271A F271A5 GCGATAACGCGGTTTAGaA 254 F271A9 GCGATAATGCGGTTTAGaA 254 G271T F271T9 GGCGATAATGCGGTTAATtA 255 A272G F272G1 GCGATAACGCGGTTTAGGgT 254 F272G9 GCGATAATGCGGTTTAGGgT 254 Reverse gyrA R GTTAGGCAGACGGCTTGGTARAATA ↵a The penultimate nucleotide to distinguish between wild-type and mutant sequences is shown in lowercase. The 1-bp mismatch at another nucleotide is underlined.
