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Journal of Clinical Microbiology
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Mycology

Multicenter Comparison of the VITEK 2 Yeast Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Fluconazole against Candida spp.

M. A. Pfaller, D. J. Diekema, G. W. Procop, M. G. Rinaldi
M. A. Pfaller
1University of Iowa College of Medicine, Iowa City, Iowa
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  • For correspondence: michael-pfaller@uiowa.edu
D. J. Diekema
1University of Iowa College of Medicine, Iowa City, Iowa
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G. W. Procop
2Cleveland Clinic Foundation, Cleveland, Ohio
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M. G. Rinaldi
3University of Texas Health Science Center, San Antonio, Texas
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DOI: 10.1128/JCM.01986-06
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ABSTRACT

A fully automated commercial antifungal susceptibility test system (VITEK 2 yeast susceptibility test; bioMerieux, Inc., Hazelwood, Mo.) was compared in three different laboratories with Clinical and Laboratory Standards Institute (CLSI) reference broth microdilution (BMD) method by testing two quality control strains and a total of 426 isolates of Candida spp. (103 to 135 clinical isolates in each laboratory plus 80 challenge isolates in one laboratory) against fluconazole. Reference BMD MIC endpoints were established after 24 and 48 h of incubation. VITEK 2 endpoints were determined spectrophotometrically after 10 to 26 h of incubation (mean, 13 h). Excellent essential agreement (within two dilutions) between the VITEK 2 and the 24- and 48-h BMD MICs was observed. The overall agreement values were 97.9 and 93.7%, respectively. Both intra- and interlaboratory agreement was 100%. The overall categorical agreement between VITEK 2 and BMD was 97.2% at the 24-h BMD time point and 88.3% at the 48-h BMD time point. Decreased categorical agreement at 48 h was attributed to trailing growth observed with Candida glabrata. The VITEK 2 system reliably detected fluconazole resistance among Candida spp. and demonstrated excellent quantitative and qualitative agreement with the reference BMD method.

  • Copyright © 2007 American Society for Microbiology
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Multicenter Comparison of the VITEK 2 Yeast Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Fluconazole against Candida spp.
M. A. Pfaller, D. J. Diekema, G. W. Procop, M. G. Rinaldi
Journal of Clinical Microbiology Mar 2007, 45 (3) 796-802; DOI: 10.1128/JCM.01986-06

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Multicenter Comparison of the VITEK 2 Yeast Susceptibility Test with the CLSI Broth Microdilution Reference Method for Testing Fluconazole against Candida spp.
M. A. Pfaller, D. J. Diekema, G. W. Procop, M. G. Rinaldi
Journal of Clinical Microbiology Mar 2007, 45 (3) 796-802; DOI: 10.1128/JCM.01986-06
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KEYWORDS

antifungal agents
Candida
fluconazole

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