Article Figures & Data
Figures
- FIG. 1.
M. genitalium strain R6G grown in Vero cell culture from a urethral swab specimen from a Danish man was added to urine or SP4 mycoplasma medium kept at room temperature or at 4°C. At 1, 2, 4, 7, 14, and 21 days of incubation, cells from 1.9 ml of urine or SP4 mycoplasma medium were harvested and frozen at −80°C. Each specimen was inoculated into Vero cell cultures, and growth was monitored by quantitative PCR. (A and B) R6G in urine at 4°C (A) and room temperature (B). (C) R6G in SP4 medium at room temperature.
Tables
- TABLE 1.
First-void urine specimens cultured for M. genitalium
Specimen name Detection of microorganism from urine DNA load of M. genitalium (geq/ml) First day of confirmed M. genitalium growth Final name M. genitalium C. trachomatis N. gonorrhoeae U. urealyticum U. parvum Urine Washed inoculum Vero cells FB medium Friis' agar R53G + − − + − 1,742,520 68,760 70 161 189 M6287 R69G + + + + + 62,520 9,160 60 76 80 M6284 R70G + − − − − 429,400 12,1800 74 80 73 M6282 R71G + + − − + 180,200 41,560 R72G + − + − − 1,232,760 3,800 R74G + − − − − 506,120 51,280 35 73 80 M6283 - TABLE 2.
Inhibition rates of selective antibiotics at different concentrations on a selection of M. genitalium strainsa
Strain % Inhibition at antibiotic concnb: Ampicillin (μg/ml) Penicillin G (IU/ml) Amphotericin B (μg/ml) Polymyxin B (μg/ml) 500 250 125 500 250 125 25 12.5 6.3 500 250 125 G37T 63.2 6.2 9.7 0 20.0 4.5 44.6 0 0 29.1 2.7 NTc M30 67.1 23.0 41.7 44.2 25.5 12.5 95.2 13.4 17.1 79.9 53.6 NT M2282 51.8 3.18 NT 27.6 0 NT 0 0 NT 0 0 NT M2300 64.9 33.6 32.7 33.6 12.4 18.0 65.3 10.0 8.8 31.0 19.2 NT M2321 33.1 21.8 0 3.3 0 0 6.0 7.8 23.3 55.0 67.0 NT M2341 94.6 31.6 68.4 42.0 59.9 21.8 39.5 47.4 10.1 62.3 44.3 NT M6090 76.1 0 0 0 0 0 95.5 0 0 0 0 NT M6151 69.3 61.6 26.8 69.6 48.4 0 100 0 0 29.5 0 NT R5G (M6280) 96.7 25.8 0 42.3 46.7 35.9 100 67.3 63.9 70.2 58.3 NT R6G (M6281) 99.7 92.1 0 99.5 91.8 0 99.9 62.4 35.4 19.5 24.0 0 R65G (M6285) 99.9 79.6 37.8 99.1 7.1 16.4 100 72.5 0 83.0 0 NT R66G (M6328) 99.9 96.9 94.3 99.9 56.9 28.3 99.9 99.9 76.9 98.2 0 NT R67G (M6286) 99.4 95.8 73.2 94.9 0 0 99.5 71.0 0 0 0 NT ↵ a M. genitalium strains were grown in Vero cell suspensions with selective antibiotics at different concentrations in wells of flat-bottom tissue culture plates at 37°C in 5% CO2. The M. genitalium DNA load in the supernatant was determined by quantitative real-time PCR 21 days after the inoculation of the M. genitalium strains.
↵ b The inhibition rate was calculated by the following formula: inhibition rate (%) = [(average of DNA loads in control wells − DNA loads in test well)/(average of DNA loads in control wells)] × 100.
↵ c NT, not tested.
