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Journal of Clinical Microbiology
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Mycobacteriology and Aerobic Actinomycetes

Evaluation and Strategy for Use of MIRU-VNTRplus, a Multifunctional Database for Online Analysis of Genotyping Data and Phylogenetic Identification of Mycobacterium tuberculosis Complex Isolates

Caroline Allix-Béguec, Dag Harmsen, Thomas Weniger, Philip Supply, Stefan Niemann
Caroline Allix-Béguec
1Genoscreen, 1, rue du Professeur Calmette, Lille 59019 Cedex, France
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Dag Harmsen
2Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, Münster D-48149, Germany
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Thomas Weniger
2Department of Periodontology, University Hospital Münster, Waldeyerstrasse 30, Münster D-48149, Germany
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Philip Supply
4INSERM U629
5Institut Pasteur de Lille, 1, rue du Professeur Calmette, Lille 59019 Cedex, France
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  • For correspondence: philip.supply@pasteur-lille.fr
Stefan Niemann
3Forschungszentrum Borstel, National Reference Center for Mycobacteria, Parkallee 1-40, Borstel 23845, Germany
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DOI: 10.1128/JCM.00540-08
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ABSTRACT

Because of its portable data, discriminatory power, and recently proposed standardization, mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing has become a major method for the epidemiological tracking of Mycobacterium tuberculosis complex (MTBC) clones. However, no public MIRU-VNTR database based on well-characterized reference strains has been available hitherto for easy strain identification. Therefore, a collection of 186 reference strains representing the primary MTBC lineages was used to build a database, which is freely accessible at http://www.MIRU-VNTRplus.org . The geographical origin and the drug susceptibility profile of each strain were stored together with comprehensive genetic lineage information, including the 24-locus MIRU-VNTR profile, the spoligotyping pattern, the single-nucleotide- and large-sequence-polymorphism profiles, and the IS6110 restriction fragment length polymorphism fingerprint. Thanks to flexible import functions, a single or multiple user strains can be analyzed, e.g., for lineage identification with or without the use of reference strains, by best-match or tree-based analyses with single or combined marker data sets. The results can easily be exported. In the present study, we evaluated the database consistency and various analysis parameters both by testing the reference collection against itself and by using an external population-based data set comprising 629 different strains. Under the optimal conditions found, lineage predictions based on typing by 24-locus MIRU-VNTR analysis optionally combined with spoligotyping were verified in >99% of the cases. On the basis of this evaluation, a user strategy was defined, which consisted of best-match analysis followed, if necessary, by tree-based analysis. The MIRU-VNTRplus database is a powerful tool for high-resolution clonal identification and has little equivalent in terms of functionalities among the bacterial genotyping databases available so far.

  • Copyright © 2008 American Society for Microbiology
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Evaluation and Strategy for Use of MIRU-VNTRplus, a Multifunctional Database for Online Analysis of Genotyping Data and Phylogenetic Identification of Mycobacterium tuberculosis Complex Isolates
Caroline Allix-Béguec, Dag Harmsen, Thomas Weniger, Philip Supply, Stefan Niemann
Journal of Clinical Microbiology Aug 2008, 46 (8) 2692-2699; DOI: 10.1128/JCM.00540-08

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Evaluation and Strategy for Use of MIRU-VNTRplus, a Multifunctional Database for Online Analysis of Genotyping Data and Phylogenetic Identification of Mycobacterium tuberculosis Complex Isolates
Caroline Allix-Béguec, Dag Harmsen, Thomas Weniger, Philip Supply, Stefan Niemann
Journal of Clinical Microbiology Aug 2008, 46 (8) 2692-2699; DOI: 10.1128/JCM.00540-08
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KEYWORDS

Bacterial Typing Techniques
DNA, Bacterial
Databases, Genetic
Interspersed Repetitive Sequences
molecular epidemiology
Mycobacterium tuberculosis
tuberculosis

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