Cross-Sectional and Longitudinal Multilocus Sequence Typing of Pseudomonas aeruginosa in Cystic Fibrosis Sputum Samples

DOI: 10.1128/JCM.00459-09

Article Figures & Data

TABLE 1.

Allelic profiles of isolates from the clock speed study (West Midlands patients only)

Patient	Time (wk)	ST	Allele^a
Patient	Time (wk)	ST	acsA	aroE	guaA	mutL	nuoD	ppsA	trpE
W01	0	148	17	5	1	3	13	6	7
	12.3	148	17	5	1	3	13	6	7
	0	170	36	5	29	7	4	10	7
	19.9	170	36	5	29	7	4	10	7
W02	0	347	40	5	17	5	4	15	7
	0	348	22	20	11	3	3	3	7
	20.1	348	22	20	11	3	3	3	7
	0	353	88	27	28	3	4	13	7
	4.1	353	88	27	28	3	4	13	7
W03	0	146	6	5	11	3	4	23	1
	12.1	146	6	5	11	3	4	23	1
W04	0	148	17	5	1	3	13	6	7
	18.3	148	17	5	1	3	13	6	7
	0	170	36	5	29	7	4	10	7
	18.1	170	36	5	29	7	4	10	7
W05	0	148	17	5	1	3	13	6	7
	5.7	148	17	5	1	3	13	6	7
W06	0	146	6	5	11	3	4	23	1
	10.9	146	6	5	11	3	4	23	1
	0	236	28	5	1	5	4	32	10
	0	349	89	30	64	78	48	24	32
	13.9	349	89	30	64	78	48	24	32
W07	0	146	6	5	11	3	4	23	1
	1	146	6	5	11	3	4	23	1
	0	148	17	5	1	3	13	6	7
	1.4	148	17	5	1	3	13	6	7
	0	236	28	5	1	5	4	32	10
	17.9	236	28	5	1	5	4	32	10
W08	0	148	17	5	1	3	13	6	7
	11.3	148	17	5	1	3	13	6	7
W09	0	349	89	30	64	78	48	24	32
	2	349	89	30	64	78	48	24	32
W10	0	146	6	5	11	3	4	23	1
	7	146	6	5	11	3	4	23	1
	0	148	17	5	1	3	13	6	7
W11	0	350	16	5	5	74	2	10	10
	7.7	350	16	5	5	74	2	10	10
W12	0	237	85 (1)	1	59	6	1	33	42
	0	238	5	1	59	6	1	33	42
	9.1	238	5	1	59	6	1	33	42
W13	0	354	16	5	5	79	2	10	10
	7.3	354	16	5	5	79	2	10	10
W14	0	148	17	5	1	3	13	6	7
	0	148-ins	17	5	1	3	13	6-ins ^b (4)	7
	8.1	148-ins	17	5	1	3	13	6-ins (4)	7
	0	352	107	3	20	62	4	7	3
	3.9	352	107	3	20	62	4	7	3
W15	0	148	17	5	1	3	13	6	7
	0	367	36	53	29	7	4	10	7
	13.9	367	36	53	29	7	4	10	7
W16	0	351	106	66	11	3	29	4	9
	6.9	351	106	66	11	3	29	4	9
W17	0	146	6	5	11	3	4	23	1
	5.6	146	6	5	11	3	4	23	1
W18	0	146	6	5	11	3	4	23	1
	12	146	6	5	11	3	4	23	1
	0	148	17	5	1	3	13	6	7
	12	148	17	5	1	3	13	6	7
W19	0	148	17	5	1	3	13	6	7
	7.3	148	17	5	1	3	13	6	7
W20	0	146	6	5	11	3	4	23	1
	2.9	146	6	5	11	3	4	23	1
	0	374	6	5	11	81 (1)	4	23	1
	2.9	374	6	5	11	81 (1)	4	23	1
W21	0	17	11	5	1	7	9	4	7
	18.7	17	11	5	1	7	9	4	7
	0	370	6	5	36	63	4	49	2
	9.3	370	6	5	36	63	4	49	2
W22	0	170-del	36	5	29	7-del ^c	4	10	7
	7.7	170-del	36	5	29	7-del	4	10	7
W23	0	146	6	5	11	3	4	23	1
	0	231	6	5	36	63	4	30	2
W24	0	148	17	5	1	3	13	6	7
	11.3	148	17	5	1	3	13	6	7
	0	231	6	5	36	63	4	30	2
	9.1	231	6	5	36	63	4	30	2
W25	0	231	6	5	36	63	4	30	2
	14.1	231	6	5	36	63	4	30	2
	0	376	6	5	36	63	4	30	59 (1)
W26	0	366	87	70	79	80	53	48	1
	0	368	90 (1)	70	79	80	53	48	1
	9.6	368	90 (1)	70	79	80	53	48	1
	0	372	90	70	29 (5)	80	53	48	1

↵ a The number of nucleotide differences between alleles of one ST and another ST is indicated in parentheses (e.g., in patient W12 acsA85 differs from acsA5 by one nucleotide).
↵ b 6-ins is the same as ppsA6 but with a 4-bp insertion.
↵ c mutL7-del is the same as mutL7 but with a 52-bp deletion from bp 26 to 77.

TABLE 2.

Allelic profiles of the STs found in the cross-sectional study of patient sputum samples

Patient	ST^a	Proportion of ST	Allele^b
Patient	ST^a	Proportion of ST	acsA	aroE	guaA	mutL	nuoD	ppsA	trpE
W03	146	35/35	6	5	11	3	4	23	1
W04	148	34/34	17	5	1	3	13	6	7
W10	146	39/39	6	5	11	3	4	23	1
W27	179	31/31	36	27	28	3	4	13	7
W28	232	36/36	17	5	11	64	4	4	2
W29	148	30/30	17	5	1	3	13	6	7
W30	148	27/27	17	5	1	3	13	6	7
W31	146	32/32	6	5	11	3	4	23	1
W01	148 (c)	8/43	17	5	1	3	13	6	7
	170	35/43	36 (5)	5	29 (3)	7 (4)	4 (2)	10 (4)	7
W07	148	11/46	17 (1)	5	1	3 (5)	13 (2)	6 (5)	7 (2)
	236 (c)	31/46	28	5	1	5	4	32	10
	239	1/46	28	5	1	5	43 (1)	32	10
	240	3/46	28	5	1	5	4	32	43 (1)
W12	238 (c)	1/32	5	1	59	6	1	33	42
	237	30/32	85 (1)	1	59	6	1	33	42
	243	1/32	86 (2)	1	59	6	1	33	42
W18	146 (c)	18/42	6	5	11	3	4	23	1
	231	24/42	6	5	36 (1)	63 (1)	4	30 (4)	2 (2)
W22	146 (c)	4/28	6	5	11	3	4	23	1
	170-del	24/28	36 (2)	5	29 (1)	7-del ^c	4	10 (3)	7 (1)
W23	146 (c)	30/35	6	5	11	3	4	23	1
	231	5/35	6	5	36 (1)	63 (1)	4	30 (4)	2 (2)
W24	148 (c)	19/30	17	5	1	3	13	6	7
	231	11/30	6 (7)	5	36 (1)	63 (1)	4 (2)	30 (5)	2 (1)
W32	148 (c)	30/37	17	5	1	3	13	6	7
	233	7/37	16 (5)	5	30 (1)	11 (3)	13	31 (5)	41 (1)

↵ a c, comparator ST.
↵ b The difference between alleles of one ST and its comparator ST is indicated in parentheses (e.g., in patient W07 nuoD43 in ST-239 differs from nuoD4 in ST-236 by one nucleotide).
↵ c mutL7-del is the same as mutL7 but with a 52-bp deletion from bp 26 to 77.