Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Bacteriology

Cholera Outbreaks Caused by an Altered Vibrio cholerae O1 El Tor Biotype Strain Producing Classical Cholera Toxin B in Vietnam in 2007 to 2008

Binh Minh Nguyen, Je Hee Lee, Ngo Tuan Cuong, Seon Young Choi, Nguyen Tran Hien, Dang Duc Anh, Hye Ri Lee, M. Ansaruzzaman, Hubert P. Endtz, Jongsik Chun, Anna Lena Lopez, Cecil Czerkinsky, John D. Clemens, Dong Wook Kim
Binh Minh Nguyen
1National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Je Hee Lee
2International Vaccine Institute, Seoul, Republic of Korea
3School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ngo Tuan Cuong
1National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Seon Young Choi
2International Vaccine Institute, Seoul, Republic of Korea
3School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nguyen Tran Hien
1National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dang Duc Anh
1National Institute of Hygiene and Epidemiology, Hanoi, Vietnam
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hye Ri Lee
2International Vaccine Institute, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. Ansaruzzaman
4International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hubert P. Endtz
4International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jongsik Chun
2International Vaccine Institute, Seoul, Republic of Korea
3School of Biological Sciences, Seoul National University, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anna Lena Lopez
2International Vaccine Institute, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cecil Czerkinsky
2International Vaccine Institute, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John D. Clemens
2International Vaccine Institute, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dong Wook Kim
2International Vaccine Institute, Seoul, Republic of Korea
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: dwkim@ivi.int
DOI: 10.1128/JCM.02040-08
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Genetic map of the CTX prophage and RS1 element in V. cholerae O1 strains. For comparison, the CTX prophage and the RS1 element on each chromosome of the El Tor reference strain N16961 (6), the classical biotype reference strain O395 (3), the Mozambican strain B33 (8), and the presumed genetic map of Vietnamese strain are shown. Locations of primers and the sequenced regions with GenBank accession numbers are shown on the map of the Vietnamese strain. Black triangles represents the CTX phage integration site. Superscript terms cla and ET refer to the classical and El Tor type genes, respectively. TLC, toxin-linked cryptic plasmid.

Tables

  • Figures
  • TABLE 1.

    Biotype characterization of V. cholerae O1 isolates from Vietnam

    Strain(s)Voges-Proskauer test resultaSensitivity to polymyxin B (50 IU)brstR typectxB typeMLST typec
    Vietnamese strains (n = 70)+REl TorClassical1,1,1,2,1,1,1,1,1
    O395 (classical)−SClassicalClassical1,1,12,2,3,1,1,6,1
    N16961 (El Tor)+REl TorEl Tor1,1,1,2,1,1,1,1,1
    B33 strain+RClassicalClassical1,1,1,2,1,1,1,1,1
    • ↵ a +, positive; −, negative.

    • ↵ b R and S indicate resistance and sensitivity, respectively.

    • ↵ c MLST loci in the order dnaE, lap, rstA, gmd, recA, pgm, gry, chi, and cat.

  • TABLE 2.

    Primer sequences used in this studya

    PrimerSequenceReference(s)
    Ch1FGACCACTCAGGCCGCTGAAAT 6
    Ch1RCCGCGCTCAAGTGGTTATCGG 6
    Ch2FAACAACAGGTTGCAAGAGAGCATT 6
    Ch2RTATTGCTTTTTTAATGGCCGTT 6
    rstRclaFTTTGCTACTTCTTCTTGGTT 11
    rstRETFTGAGCATAAGCTCTTGATTT 6, 11
    rstARCCGTGAAAGTCATCAACG 6
    rstCFGATGTTTACGATAGCCTAGAAGACTT 6
    rstCRTACAGTGATGGCTCAGTCAATGC 6
    rstCF4AAATCCGCAACTCAAGGCATTGA 6
    rstCR4TAAGCGCCTGAACGCAGATATAAAG 6
    ctxBFAGATATTTTCGTATACAGAATCTCTAG 6
    cepRAAACAGCAAGAAAACCCCGAGT 6
    rstRCalFTCAAGCTTTTTTTTGCTTTATCTTA 2, 11
    rstRCalRTGGCAACAAAGCACATTAAAGA 2, 11
    rstREnvFGCTTCATTTGTGTATTGGTCTATTAGGTAGTTA 11
    rstREnvRTCGAGTTGTAATTCATCAAGAGTGAAAA 11
    • ↵ a Primers were designed in this study based on the sequences described in the references.

PreviousNext
Back to top
Download PDF
Citation Tools
Cholera Outbreaks Caused by an Altered Vibrio cholerae O1 El Tor Biotype Strain Producing Classical Cholera Toxin B in Vietnam in 2007 to 2008
Binh Minh Nguyen, Je Hee Lee, Ngo Tuan Cuong, Seon Young Choi, Nguyen Tran Hien, Dang Duc Anh, Hye Ri Lee, M. Ansaruzzaman, Hubert P. Endtz, Jongsik Chun, Anna Lena Lopez, Cecil Czerkinsky, John D. Clemens, Dong Wook Kim
Journal of Clinical Microbiology Apr 2009, 47 (5) 1568-1571; DOI: 10.1128/JCM.02040-08

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Cholera Outbreaks Caused by an Altered Vibrio cholerae O1 El Tor Biotype Strain Producing Classical Cholera Toxin B in Vietnam in 2007 to 2008
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Cholera Outbreaks Caused by an Altered Vibrio cholerae O1 El Tor Biotype Strain Producing Classical Cholera Toxin B in Vietnam in 2007 to 2008
Binh Minh Nguyen, Je Hee Lee, Ngo Tuan Cuong, Seon Young Choi, Nguyen Tran Hien, Dang Duc Anh, Hye Ri Lee, M. Ansaruzzaman, Hubert P. Endtz, Jongsik Chun, Anna Lena Lopez, Cecil Czerkinsky, John D. Clemens, Dong Wook Kim
Journal of Clinical Microbiology Apr 2009, 47 (5) 1568-1571; DOI: 10.1128/JCM.02040-08
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • rstR type.
    • Presence and the location of rstC (RS1).
    • Absence of the tandem repeat of CTX prophage and RS1.
    • Location of RS1-CTX prophage array.
    • Toxin type determination.
    • Multilocus sequence typing (MLST) analysis.
    • Nucleotide sequence accession numbers.
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Cholera
Cholera Toxin
Disease Outbreaks
Vibrio cholerae O1

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X