Article Figures & Data
Figures
- FIG. 1.
Genetic map of the CTX prophage and RS1 element in V. cholerae O1 strains. For comparison, the CTX prophage and the RS1 element on each chromosome of the El Tor reference strain N16961 (6), the classical biotype reference strain O395 (3), the Mozambican strain B33 (8), and the presumed genetic map of Vietnamese strain are shown. Locations of primers and the sequenced regions with GenBank accession numbers are shown on the map of the Vietnamese strain. Black triangles represents the CTX phage integration site. Superscript terms cla and ET refer to the classical and El Tor type genes, respectively. TLC, toxin-linked cryptic plasmid.
Tables
- TABLE 1.
Biotype characterization of V. cholerae O1 isolates from Vietnam
Strain(s) Voges-Proskauer test resulta Sensitivity to polymyxin B (50 IU)b rstR type ctxB type MLST typec Vietnamese strains (n = 70) + R El Tor Classical 1,1,1,2,1,1,1,1,1 O395 (classical) − S Classical Classical 1,1,12,2,3,1,1,6,1 N16961 (El Tor) + R El Tor El Tor 1,1,1,2,1,1,1,1,1 B33 strain + R Classical Classical 1,1,1,2,1,1,1,1,1 - TABLE 2.
Primer sequences used in this studya
Primer Sequence Reference(s) Ch1F GACCACTCAGGCCGCTGAAAT 6 Ch1R CCGCGCTCAAGTGGTTATCGG 6 Ch2F AACAACAGGTTGCAAGAGAGCATT 6 Ch2R TATTGCTTTTTTAATGGCCGTT 6 rstRclaF TTTGCTACTTCTTCTTGGTT 11 rstRETF TGAGCATAAGCTCTTGATTT 6, 11 rstAR CCGTGAAAGTCATCAACG 6 rstCF GATGTTTACGATAGCCTAGAAGACTT 6 rstCR TACAGTGATGGCTCAGTCAATGC 6 rstCF4 AAATCCGCAACTCAAGGCATTGA 6 rstCR4 TAAGCGCCTGAACGCAGATATAAAG 6 ctxBF AGATATTTTCGTATACAGAATCTCTAG 6 cepR AAACAGCAAGAAAACCCCGAGT 6 rstRCalF TCAAGCTTTTTTTTGCTTTATCTTA 2, 11 rstRCalR TGGCAACAAAGCACATTAAAGA 2, 11 rstREnvF GCTTCATTTGTGTATTGGTCTATTAGGTAGTTA 11 rstREnvR TCGAGTTGTAATTCATCAAGAGTGAAAA 11 ↵ a Primers were designed in this study based on the sequences described in the references.
