DOI: 10.1128/JCM.02141-08
Article Figures & Data
Figures
- FIG. 1.
Pulsed-field gel electrophoresis (XbalI) of whole genomic DNA.
Tables
- TABLE 1.
Bacterial strains used in this study
Isolate Detail MIC (μg/ml)a TIM CTX IPM GEN JIE515 Serratia sp. strain >64/2 >32 4 >8 JIE527 E. coli blood culture isolate from day 30 16/2 <2 <1 <2 JIE528 E. coli blood culture isolate from day 31 >64/2 >32 <1 >8 DH5α E. coli DH5αRif (laboratory strain, no plasmid) 8/2 4 <1 <2 pJIBE401 E. coli DH5αRif with pJIBE401 (IMP-4 plasmid) >64/2 32 2 >8 ↵ a Abbreviations: TIM, ticarcillin-clavulanic acid; CTX, ceftriaxone; IPM, imipenem; GEN, gentamicin. For all of the strains, the tobramycin MIC equaled the gentamicin MIC, the amikacin MIC was <8 μg/ml, and the ciprofloxacin MIC was ≤1 μg/ml.
- TABLE 2.
Conjugative transfer of plasmids
Plasmid marker Conjugative transfera from: Serratia JIE515 E. coli JIE527 E. coli JIE528 bla IMP-4 + + IncL/M + + IncHI2 ± IncN (+) (+) IncFIA (+) (+) IncFII (+) (+) ↵ a Conjugative transfer of blaIMP-4 and plasmids of Inc groups to E. coli DH5α was determined as previously described (1). Symbols: +, always; ±, sometimes; (+), present in wild-type strain but not transferred.
