Use of a New Single Multiplex PCR-Based Assay for Direct Simultaneous Characterization of Six Neisseria meningitidis Serogroups

Multiplex PCR amplification of the siaD/synB and siaD/synE (serogroups B and C), synG and synF (serogroups W135 and Y), orf-2 (serogroup A), and ctrA (serogroup X) genes from samples belonging to serogroup A (lanes 1 to 8), B (lanes 9 to 17), C (lanes 18 to 21) (A) and X (lanes 22 to 31) and W135 (lanes 32 to 43) and Y (lanes 44 to 50) (B). DNA from other species, Haemophilus influenzae (lanes 51, 52, 54, 55, 57) and Streptococcus pneumoniae (lanes 53, 56, 58, 59, and 60), were used as negative controls. PCR products were separated on a 4% agarose gel. Molecular sizes are indicated.