DOI: 10.1128/JCM.00599-09
Article Figures & Data
Figures
- FIG. 1.
Real-time RT-PCR results for serial dilutions of RNAs of BTV-1, -6, and -8 with the appropriate primer-probe mixtures. For BTV-1 and BTV-6, the alternative mixtures produced similar results (BTV1-VP2-Mix2-FAM, y = −3.4471x + 17.383 [R2 = 0.9983]; BTV6-VP2-Mix2-FAM, y = −3.2771x + 8.5275 [R2 = 0.999]; data not shown).
Tables
- TABLE 1.
Primers and probes used in this study
Oligonucleotide (product size [bp]) and type Name Sequence BTV1-VP2-Mix1-FAM (123) Forward primer BTV1-VP2-186F 5′ CGG ACC GCA TTA TGG TAT AAC C 3′ Reverse primer BTV1-VP2-308R 5′ ACT CTT GTG TCT CGT ACT TTC AAC 3′ Probe BTV1-VP2-203FAM 5′ ACC GCC CGT CTT TCA TCG TAA CCC 3′ BTV1-VP2-Mix2-FAM (120) Forward primer BTV1-VP2-2407F 5′ CCT CAA AGG CGA TTC GAT TTA GC 3′ Reverse primer BTV1-VP2-2526R 5′ TCA CGA CGT TGT AGT TGA CTC C 3′ Probe BTV1-VP2-2438FAM 5′ TGA AGC GCA GCC CAA GAT TGC ACG 3′ BTV6-VP2-Mix1-FAM (97) Forward primer BTV6-VP2-785F 5′ GAT ACG TGA TGC GTG GAT TG 3′ Reverse primer BTV6-VP2-881R 5′ TAC CAC CTT CCT TCC GAC AC 3′ Probe BTV6-VP2-817FAM 5′ ATC CGA GGC ATA TTC GCT CGC TGG 3′ BTV6-VP2-Mix2-FAM (89) Forward primer BTV6-VP2-1056F 5′ TAT AAT GGC AGA ATA TGG TGG AC 3′ Reverse primer BTV6-VP2-1144R 5′ CAG TAA ACA TCG CCC AAC CT 3′ Probe BTV6-VP2-1081FAM 5′ ATC CGT ACC CTT GCT TGC GTG GAG 3′ BTV8-VP2-Mix1-FAM (86) Forward primer BTV8-VP2-1604F 5′ GTT ACG CAT TAC CGA GGT TGT G 3′ Reverse primer BTV8-VP2-1689R 5′ GAT CAT GTG TGA ACG CCT TCG 3′ Probe BTV8-VP2-1631FAM 5′ AAC GGC TCA CAC CGA CGA TCC AGC 3′
Supplemental material
Files in this Data Supplement:
- Supplemental file 1
-
Table S1 (Real-time RT-PCR results for clinical samples submitted to the NRL and samples from in-house animal experiments).
MS Word document, 30K.
- Supplemental file 1
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Table S1 (Real-time RT-PCR results for clinical samples submitted to the NRL and samples from in-house animal experiments).
