Virology

Prevalence and Management of Invalid GeneXpert Enterovirus Results Obtained with Cerebrospinal Fluid Samples: a 2-Year Study

Susan E. Sefers, Anna K. Raymer, Jessica T. Kilby, David H. Persing, Yi-Wei Tang
DOI: 10.1128/JCM.01238-09

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  • TABLE 1.

    Invalid GXEA results correlate to levels of visible RBCs in CSF specimens

    Visible RBC rankingNo. of specimensNo. (%) of specimens with invalid resultInvalid-result odds ratioaNo. (%) of EnV-positive specimensbEnV- positive odds ratio
    None38819 (4.9)1.0081 (20.9)1.00
    1+573 (5.3)1.078 (14.0)0.67
    2+355 (14.3)2.928 (22.9)1.10
    3+268 (38.8)6.286 (23.1)1.11
    4+198 (42.1)8.603 (15.8)0.76

    • ↵ a χ2 trend = 38.01, P < 0.0001.

    • ↵ b Includes those specimens that previously tested invalid.

  • TABLE 2.

    A 1:5 dilution of fresh specimens or a one-time freeze-thaw of specimens diminishes invalid results without decreasing test sensitivity

    Method for diminishing invalid resultsNo. of invalid specimens treatedNo. invalid after use of method% Diminishment of invalid-result rateaNo. of positive specimens treatedNo. positive after use of method% Sensitivity after use of methodb
    1:5 dilution28582.1585798.3
    Freeze-thaw25196.0555396.4

    • ↵ a There was no significant difference in rates of diminishment of invalid results for dilution and freeze-thawing; Fisher's exact test, P > 0.05.

    • ↵ b There was no significant difference in sensitivity of GXEA after dilution and freeze-thawing; Fisher's exact test, P > 0.05.

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KEYWORDS

cerebrospinal fluid
Diagnostic Errors
Enterovirus Infections
Molecular Diagnostic Techniques
Reagent Kits, Diagnostic

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