DOI: 10.1128/JCM.00687-09
Article Figures & Data
Tables
- TABLE 1.
Reference strains removed from the database library due to discordant identification results or unacceptable spectra
Reference organism Identification a Sequencing MALDI-TOF Candida periphelosum ATCC 20314 Candida guilliermondii Candida guilliermondii Candida periphelosum ATCC 20317 Candida guilliermondii Candida guilliermondii Candida rugosa ATCC 96275 Candida pararugosa Candida pararugosa Candida zeylanoides ATCC 15585 Candida guilliermondii Candida guilliermondii Candida zeylanoides ATCC 20393 Candida lipolytica Candida lipolytica Cryptococcus luteolus ATCC 44440 ND No acceptable spectra Cryptococcus luteolus ATCC 42279 Cryptococcus podzolicus No acceptable spectra Cryptococcus uniguttulatus ATCC 34143 Candida glabrata Candida glabrata Geotrichum candidum ATCC 10834 ND No acceptable spectra Geotrichum candidum ATCC 74169 ND No acceptable spectra Malassezia sympodialis ATCC 96803 ND No acceptable spectra Trichosporon asahii UMAH 10278 Trichosporon asteroides Trichosporon asteroides Trichosporon ovoides UMAH 563 Trichosporon asahii Trichosporon ovoides/asahii Trichosporon ovoides UMAH 672 Trichosporon asahii Trichosporon ovoides/asahii Trichosporon ovoides UMAH 635 Trichosporon asahii/faecale Trichosporon faecale ↵ a ND, not done.
- TABLE 2.
Reference strains and gene sequence-confirmed NIH clinical isolates evaluated for inclusion in the MALDI-TOF MS fungal database library
Reference isolate Strain(s) Candida albicans ATCC 18804T, NIH 513, NIH 528, NIH 540 Candida catenulata ATCC 10565T, ATCC 20117, ATCC 22872, ATCC 42214, ATCC 200552 Candida dubliniensis ATCC MYA-646T, ATCC MYA-583, ATCC MYA-579, ATCC MYA- 580, ATCC MYA-582 Candida glabrata ATCC 2001T, NIH 517, NIH 555, NIH 546, NIH 512 Candida guilliermondii ATCC 6260T Candida haemulonii ATCC 22991T Candida kefyr ATCC 90902, ATCC 204093, ATCC 42265T, ATCC 200553, ATCC 64885 Candida krusei ATCC 6258T Candida lambica ATCC 24750T Candida lipolytica ATCC 18943T, ATCC 18942, ATCC 20776, ATCC 20496, ATCC 201089 Candida lusitaniae ATCC 34449T, NIH 526 Candida metapsilosis ATCC 96144T Candida orthopsilosis ATCC 96139T Candida parapsilosis ATCC 22019T, NIH 521, NIH 534 Candida pararugosa ATCC 38774T Candida periphelosum ATCC 36239 Candida pintolopesii ATCC 22987T, ATCC 22998, ATCC 36231, ATCC 64177, ATCC 66058 Candida rugosa ATCC 200555, ATCC 238772, ATCC 58964, ATCC 10571T, NIH 574 Candida tropicalis ATCC 750T, NIH 515, NIH 536 Candida zeylanoides ATCC 26899, ATCC 7351T, ATCC 96278 Cryptococcus laurentii ATCC 18803T, ATCC 34142, ATCC 36833, ATCC 26024, ATCC 10668 Cryptococcus luteolus ATCC 32044T Cryptococcus gattii ATCC 32269T Cryptococcus neoformans ATCC 32268, ATCC 14248, ATCC 32267, ATCC 56991, ATCC 32045T, ATCC 208821 Cryptococcus podzolicus ATCC 34208T Cryptococcus terreus ATCC 11799T, ATCC 32046, ATCC 32422, ATCC 34145 Cryptococcus uniguttulatus ATCC 66033, ATCC 38298, ATCC 32047T, ATCC 32048 Geotrichum candidum ATCC 22601T, ATCC 204307 Geotrichum capitatum ATCC 10663T, ATCC 62964 Kodamaea ohmeri ATCC 26861T Malassezia furfur ATCC 96809T Malassezia pachydermatis ATCC 14522T Pichia anomala ATCC 8168T Rhodotorula glutinis ATCC 16726T Rhodotorula mucilaginosa ATCC 11799T Trichosporon asahii ATCC 90039T, UAMH 7654, UAMH 7655 Trichosporon asteroides ATCC 90043T, UAMH 4261, UAMH 7656 Trichosporon coremiiforme UAMH 7658, ATCC 90042T Trichosporon faecale ATCC 90041T Trichosporon inkin ATCC 18020T, UAMH 4260, UAMH 7663, UAMH 7664 Trichosporon jirovecii ATCC 34499T Trichosporon moniliiforme ATCC 90045 Trichosporon mucoides ATCC 90046, UAMH 7670 Trichosporon ovoides ATCC 90040, UAMH 7671 - TABLE 3.
Identification of clinical isolates by MALDI-TOF MS
Organism (no. of isolates tested) No. of isolates with spectral score of: <1.8 1.8-<1.9 1.9-<2.0 >2.0 Candida albicans (20) 20 Candida catenulata (2) 2 Candida dubliniensis (12) 12 Candida glabrata (11) 11 Candida guilliermondii (15) 3 2 10 Candida haemulonii (2) 2 Candida kefyr (10) 10 Candida krusei (9) 1 8 Candida lipolytica (9) 9 Candida lusitaniae (10) 5 3 2 Candida metapsilosis (8) 1 7 Candida orthopsilosis (21) 2 19 Candida parapsilosis (17) 17 Candida pelliculosa (10) 1 9 Candida rugosa (7) 1 6 Candida tropicalis (8) 8 Cryptococcus neoformans (6) 1 1 1 3 Geotrichum candidum (2) 2 Malasezzia pachydermatis (2) 2 Rhodotorula mucilaginosa (5) 5 Trichosporon asahii (4) 1 1 2 Trichosporon coremiiforme (1) 1 Trichosporon mucoides (3) 3 Total (194) 2 13 10 169
