Mycology

Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens

P. Lewis White, Michael D. Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J. Peter Donnelly, Rosemary A. Barnes
and on behalf of the European Aspergillus PCR Initiative
DOI: 10.1128/JCM.01466-10

ABSTRACT

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

FOOTNOTES

    • Received 20 July 2010.
    • Returned for modification 6 August 2010.
    • Accepted 10 August 2010.

  • ↵▿ Published ahead of print on 18 August 2010.

  • ↵† The authors have paid a fee to allow immediate free access to this article.

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