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Journal of Clinical Microbiology
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Mycology

Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens

P. Lewis White, Michael D. Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J. Peter Donnelly, Rosemary A. Barnes, ; on behalf of the European Aspergillus PCR Initiative
P. Lewis White
1Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom
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  • For correspondence: lewis.white@wales.nhs.uk
Michael D. Perry
1Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom
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Juergen Loeffler
2Wuerzburg University, Wuerzburg, Germany
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Willem Melchers
3Radboud University, Nijmegen Medical Centre, Nijmegen, Netherlands
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Lena Klingspor
4Karolinska University Hospital, Stockholm, Sweden
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Stephane Bretagne
5Henri Mondor Hospital, Créteil, France
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Elaine McCulloch
6Royal Hospital for Sick Children, Glasgow, Scotland, United Kingdom
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Manuel Cuenca-Estrella
7National Microbiological Institute, Madrid, Spain
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Niklas Finnstrom
8Cepheid AB, Bromma, Sweden
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J. Peter Donnelly
3Radboud University, Nijmegen Medical Centre, Nijmegen, Netherlands
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Rosemary A. Barnes
9School of Medicine, Department of Infection, Immunity and Biochemistry, Cardiff University, UHW, Heath Park, Cardiff, Wales, United Kingdom
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DOI: 10.1128/JCM.01466-10
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ABSTRACT

A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

  • Copyright © 2010 American Society for Microbiology
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Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens
P. Lewis White, Michael D. Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J. Peter Donnelly, Rosemary A. Barnes on behalf of the European Aspergillus PCR Initiative
Journal of Clinical Microbiology Sep 2010, 48 (10) 3753-3755; DOI: 10.1128/JCM.01466-10

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Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens
P. Lewis White, Michael D. Perry, Juergen Loeffler, Willem Melchers, Lena Klingspor, Stephane Bretagne, Elaine McCulloch, Manuel Cuenca-Estrella, Niklas Finnstrom, J. Peter Donnelly, Rosemary A. Barnes on behalf of the European Aspergillus PCR Initiative
Journal of Clinical Microbiology Sep 2010, 48 (10) 3753-3755; DOI: 10.1128/JCM.01466-10
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KEYWORDS

aspergillosis
Aspergillus fumigatus
blood
DNA, Fungal
mycology
polymerase chain reaction

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