Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
LETTERS TO THE EDITOR

Further Characterization of Proteolytic Clostridium botulinum Type A5 Reveals that Neurotoxin Formation Is Unaffected by Loss of the cntR (botR) Promoter Sigma Factor Binding Site

Andrew T. Carter, David R. Mason, Kathie A. Grant, Giovanna Franciosa, Paulo Aureli, Michael W. Peck
Andrew T. Carter
Institute of Food Research Foodborne Bacterial Pathogens Programme Norwich NR4 7UA, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: andrew.carter@bbsrc.ac.uk
David R. Mason
Institute of Food Research Foodborne Bacterial Pathogens Programme Norwich NR4 7UA, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kathie A. Grant
HPA Centre for Infections Foodborne Pathogens Reference Unit London NW9 5EQ, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Giovanna Franciosa
Department of Food Safety and Veterinary Public Health Istituto Superiore di Sanità Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paulo Aureli
Department of Food Safety and Veterinary Public Health Istituto Superiore di Sanità Rome, Italy
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael W. Peck
Institute of Food Research Foodborne Bacterial Pathogens Programme Norwich NR4 7UA, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JCM.01774-09
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • FIG. 1.
    • Open in new tab
    • Download powerpoint
    FIG. 1.

    Position of a 76-bp deletion identified in the neurotoxin gene cluster of proteolytic C. botulinum type A5 strain H04402 065. Neurotoxins and associated genes are shaded. Prime symbols denote a partial gene. Upper part of figure represents coding regions spanning 18.2 kb of a chromosomal DNA sequence (GenBank accession number GQ477161). The −35 and −10 binding sites for the CntR/RNA polymerase complex are indicated for each gene that is positively regulated by CntR. Alternative nomenclature for neurotoxin cluster genes is as follows: cntC (ha34), cntD (ha17), cntE (ha70), cntR (botR), cntB (ntnh), cntA/A5 (bont/A5), cntA/B3′ (bont/B3′).

  • FIG. 2.
    • Open in new tab
    • Download powerpoint
    FIG. 2.

    Growth and neurotoxin formation by proteolytic C. botulinum type A5 strain H04402 065 (squares) and type A1 strain ATCC 3502 (triangles). Closed symbols represent the viable count (left y axis); open symbols represent the neurotoxin concentration in culture supernatant (right y axis).

PreviousNext
Back to top
Download PDF
Citation Tools
Further Characterization of Proteolytic Clostridium botulinum Type A5 Reveals that Neurotoxin Formation Is Unaffected by Loss of the cntR (botR) Promoter Sigma Factor Binding Site
Andrew T. Carter, David R. Mason, Kathie A. Grant, Giovanna Franciosa, Paulo Aureli, Michael W. Peck
Journal of Clinical Microbiology Feb 2010, 48 (3) 1012-1013; DOI: 10.1128/JCM.01774-09

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Further Characterization of Proteolytic Clostridium botulinum Type A5 Reveals that Neurotoxin Formation Is Unaffected by Loss of the cntR (botR) Promoter Sigma Factor Binding Site
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Further Characterization of Proteolytic Clostridium botulinum Type A5 Reveals that Neurotoxin Formation Is Unaffected by Loss of the cntR (botR) Promoter Sigma Factor Binding Site
Andrew T. Carter, David R. Mason, Kathie A. Grant, Giovanna Franciosa, Paulo Aureli, Michael W. Peck
Journal of Clinical Microbiology Feb 2010, 48 (3) 1012-1013; DOI: 10.1128/JCM.01774-09
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Bacterial Proteins
Clostridium botulinum
Neurotoxins
Promoter Regions, Genetic
Sequence Deletion
Sigma Factor
Trans-Activators

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X