DOI: 10.1128/JCM.01227-09
Article Figures & Data
Tables
- TABLE 1.
Sequences of primers used in this study
Functional category Primer Primer sequence (5′ to 3′) Reference Phylogenetic group ChuA.1 GACGAACCAACGGTCAGGAT 2 ChuA.2 TGCCGCCAGTACCAAAGACA YjaA.1 TGAAGTGTCAGGAGACGCTG YjaA.2 ATGGAGAATGCGTTCCTCAAC TspE4C2.1 GAGTAATGTCGGGGCATTCA Toxins TspE4C2.2 CGCGCCAACAAAGTATTACG Cytotoxic necrotizing factor (cnf1) CNF1-1 CTGACTTGCCGTGGTTTAGTCGG 6 CNF1-2 TACACTATTGACATGCTGCCCGGA Hemolysin A (hlyA) hlyA.1 AACAAGGATAAGCACTGTTCTGGCT hlyA.2 ACCATATAAGCGGTCATTCCCGTCA Fimbriae and/or adhesins P-fimbrial adhesin II (papGII) papG_II f GGGATGAGCGGGCCTTTGAT 4 papG_II r CGGGCCCCCAAGTAACTCG P-fimbrial adhesin III (papGIII) papG_III f CCACCAAATGACCATGCCAGAC 15 papG_III r GGCCTGCAATGGATTTACCTGG S fimbriae (sfaDE) SFA-1 CTCCGGAGAACTGGGTGCATCTTAC 7 CGGAGGAGTAATTACAAACCTGGCA Afa/Dr adhesins (afa/draBC) afa/draBC-f GGCAGAGGGCCGGCAACAGGC 3 afa/draBC-r CCCGTAACGCGCCAGCATCTC Iron uptake Aerobactin synthesis (iucD) Aer1 TACCGGATTGTCATATGCAGACCGT 15 Aer2 AATATCTTCCTCCAGTCCGGAGAAG Other Uropathogenic strain-specific protein (usp) N6 ATGCTACTGTTTCCGGGTAGTGTGT 8 N7 CATCATGTAGTCGGGGCGTAACAAT TIR domain-containing protein (tcpC) tcpC for GGCAACAATATGTATAATATCCT tcpC rev GCCCAGTCTATTTCTGCTAAAGA 1 - TABLE 2.
Distribution of phylogenetic groups and virulence factors in relation to the presence of tcpC
Phylogenetic group or virulence factor Prevalence (no. [%] of strains)a UTI + SSTI isolates UTI isolates SSTI isolates Commensal isolates tcpC positive (49 [23]) tcpC negative (163 [77]) tcpC positive (23 [21]) tcpC negative (87 [79]) tcpC positive (26 [25]) tcpC negative (76 [75]) tcpC positive (7[8]) tcpC negative (83 [92]) Phylogenetic group A 5 (10) 35 (21) 0 (0) 28 (32)** 5 (19) 7 (9) 0 (0) 20 (24) B1 3 (6) 13 (8) 0 (0) 6 (7) 3 (12) 7 (9) 0 (0) 13 (16) B2 40 (82) 81 (50)*** 23 (100) 32 (37)*** 17 (65) 49 (64) 7 (100) 23 (28)** D 1 (2) 34 (21)** 0 (0) 21 (24)* 1 (4) 13 (17) 0 (0) 27 (33) Virulence factor cnf1 33 (67) 25 (15)*** 16 (70) 9 (10)*** 17 (65) 16 (21)** 4 (57) 1 (1)** hlyA 33 (67) 26 (16)*** 18 (78) 10 (11)*** 15 (58) 16 (21)* 5 (71) 2 (2)*** papGIII 19 (39) 10 (6)*** 11 (48) 3 (3)*** 8 (31) 7 (9) 3 (43) 0 (0)** papGII 13 (27) 34 (21) 11 (48) 26 (30) 2 (8) 8 (11) 0 (0) 7 (8) sfaDE 33 (67) 30 (18)*** 19 (83) 7 (8)*** 14 (54) 23 (30) 7 (100) 8 (10)*** afa/draBC 0 (0) 3 (2) 0 (0) 2 (2) 0 (0) 1 (1) 0 (0) 4 (5) iucD 24 (49) 70 (43) 13 (57) 33 (38) 11 (42) 37 (49) 2 (29) 33 (40) usp 44 (90) 49 (30)*** 22 (96) 26 (30)*** 22 22 (85) 23 (30)* 6 (86) 1 (1)*** Average virulence score 4.06 1.52 4.78, 1.33 3.19, 1.72 3.86, 0.67 ↵ a The P values obtained following Bonferroni correction are indicated by asterisks when P is <0.05, as follows: *, P < 0.05; **, P < 0.005; ***, P < 0.0005.
