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LETTERS TO THE EDITOR

Comparison of Bacterial Antigen Test and Gram Stain for Detecting Classic Meningitis Bacteria in Cerebrospinal Fluid

Tess Karre, Emily A. Vetter, Jayawant N. Mandrekar, Robin Patel
Tess Karre
Division of Clinical Microbiology Department of Laboratory Medicine and Pathology
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Emily A. Vetter
Division of Clinical Microbiology Department of Laboratory Medicine and Pathology
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Jayawant N. Mandrekar
Division of Biomedical Statistics and Informatics
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Robin Patel
Divisions of Clinical Microbiology and Infectious Diseases Department of Medicine Mayo Clinic 200 First St. SW Rochester, Minnesota 55905
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  • For correspondence: patel.robin@mayo.edu
DOI: 10.1128/JCM.00153-10
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Tables

  • TABLE 1.

    Comparison of BAT and Gram stain resultsa

    Gram stain resultNo. (%) of specimens
    BAT positiveBAT negative
    Positive26 (62)11 (26)
    Negative3 (7)a 2 (5)
    • ↵ a Forty-two specimens were tested. The results of the BAT and the Gram stain were not statistically significantly different from one another (P = 0.64, Fisher's exact test). All three Gram stain-negative specimens were culture positive.

  • TABLE 2.

    Specimens with and without classic bacterial meningitis-causing organisms and number not detected by BAT and Gram stain

    OrganismTotal no. of specimensNo. (%) of specimens not detected by:
    BATGram stain
    Detected by culturea
        Streptococcus pneumoniae 226 (27)3 (14)
        Group B Streptococcus species73 (43)0 (0)
        Neisseria meningitidis 73 (43)1 (14)
        Haemophilus influenzae type b20 (0)1 (50)
    Not detected by cultureb 41 (25)0 (0)
    Total4213 (31)5 (12)
    • ↵ a Growth from cerebrospinal fluid, blood, or ear drainage specimens.

    • ↵ b Results of both the BAT and the Gram stain were positive for three specimens, with results indicating N. meningitidis in two and S. pneumoniae in one. In one specimen, the Gram stain showed Gram-positive cocci resembling streptococci, the BAT was negative, and the urine pneumococcal antigen test was positive.

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Comparison of Bacterial Antigen Test and Gram Stain for Detecting Classic Meningitis Bacteria in Cerebrospinal Fluid
Tess Karre, Emily A. Vetter, Jayawant N. Mandrekar, Robin Patel
Journal of Clinical Microbiology Mar 2010, 48 (4) 1504-1505; DOI: 10.1128/JCM.00153-10

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Comparison of Bacterial Antigen Test and Gram Stain for Detecting Classic Meningitis Bacteria in Cerebrospinal Fluid
Tess Karre, Emily A. Vetter, Jayawant N. Mandrekar, Robin Patel
Journal of Clinical Microbiology Mar 2010, 48 (4) 1504-1505; DOI: 10.1128/JCM.00153-10
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KEYWORDS

Antigens, Bacterial
bacteria
bacteriological techniques
cerebrospinal fluid
Meningitis, Bacterial

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