Article Figures & Data
Figures
- FIG. 1.
Molecular characterization of 15 single colonies isolated from the original M. avium-M. kansasii mixed culture by PRA-ITS (A), PCR-IS1245 (B), RFLP-IS1245 (C), and pulsed-field gel electrophoresis (D). Lanes: colonies 88.1 to 88.4, M. avium; colonies 88.5 to 88.15, M. kansasii; −, PCR negative control (water); +, PCR positive control (DNA from M. avium ATCC 25291T). Asterisks indicate RFLP-IS1245 band polymorphisms in M. avium colonies. On the right are molecular size markers.
- FIG. 2.
RFLP-IS1245 of colonies 88.11 and 88.12 and individual colonies isolated after 10 serial passages in 7H9-OADC liquid medium. Lanes: 1, original colony 88.11; 2 to 10, isolated colonies after subculture of colony 88.11; 11, original colony 88.12; 12 to 21, isolated colonies after subculture of colony 88.12. Black arrows indicate the hybridization bands present in the original colonies. On the right are molecular size markers.
