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- TABLE 1.
Serological, blood culture, and 16S-23S intergenic spacer PCR test results for a family in which members were infected with Bartonella vinsonii subsp. berkhoffii and Bartonella henselaea
Patient, type of sample, date (mo/day/yr) Bartonella IFA reciprocal titer for: PCR/DNA sequencing result(s) for: Bvb I Bvb II Bvb III Bh Direct extraction BAPGM enrichment culture Subculture Father Blood, 4/11/2009 <16 <16 <16 <16 Neg Bvb II Neg Mother Blood, 4/11/2009 <16 <16 <16 <16 Bh SA2 Bvb II Neg Placenta, 1998 NA NA NA NA Neg NA NA Cervical biopsy specimen, 1991 NA NA NA NA Bh SA2 NA NA Son Blood, 4/11/2009 16 64 16 64 Neg Neg Neg Blood, 6/10/2009 64 1,024 256 1,024 Bh SA2b Bh/Bvbb Neg Blood, 9/9/2009 256 128 64 256 Neg Bhb Neg Daughter Brain, 1998 NA NA NA NA Bh SA2 NA NA Liver, 1998 NA NA NA NA Bh SA2/Bvb II NA NA Spleen, 1998 NA NA NA NA Neg NA NA ↵ a Bvb I, II, and III, B. vinsonii subsp. berkhoffii genotypes I, II, and III; Bh SA2, B. henselae strain SA2; NA, serology and culture not applicable (PCR testing was performed on stored paraffin-embedded tissues); Neg, negative.
↵ b These ITS PCR results were confirmed by targeting the rpoB gene, as overlapping sequences were obtained following amplification of the 16S-23S intergenic spacer region. The rpoB gene does not allow for Bartonella spp. strain discrimination; however, the partial, nonoverlapping ITS sequence confirmed an SA2 strain. All PCR results were confirmed by DNA sequencing.
