Streptococcus salivarius Meningitis Case Strain Traced to Oral Flora of Anesthesiologist

Patricia L. Shewmaker; Robert E. Gertz; Clara Y. Kim; Sietske de Fijter; Mary DiOrio; Matthew R. Moore; Bernard W. Beall

doi:10.1128/JCM.00426-10

DOI: 10.1128/JCM.00426-10

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Tables

TABLE 1.

Multilocus and 16S rRNA gene sequences depicting identical results from meningitis-associated S. salivarius isolates from 2 case patients and carriage isolates recovered from their anesthesiologist

Isolate	Allele for indicated gene^a
Isolate	glcK^b	ddlA^b	pepO^b	ilvC^b	thrS^b	pyrE^b	dnaE^b	sodA^b	rpoB^c	sodA^c	pyk^c	ppaC^c	tuf^c	pfl^c	map^c	16S rRNA gene
0735^d	4	Unique	3	4	14	12	Unique	14	Unique	Ref 3	Ref 3	Ref 3	Unique	Unique	Unique	Unique
JIM8221^e	4	3	3	4	14	12	17	14	ND	ND	ND	ND	ND	ND	ND	ND
SS908	2	ND	ND	ND	ND	ND	ND	Unique	ND	ND	ND	ND	ND	ND	ND	AF459433^f

↵ a “Unique” indicates that the sequences for the genes (the ddlA, dnaE, sodA, rpoB, tuf, pfl, map, and 16S rRNA genes) were unique to these case and carriage isolates (corresponding to GenBank accession numbers GU556184, Gu556185, GUU556186, GU556187, GU556190, GU556191, GU556192, and GU175444, respectively). “Ref 3” indicates sequences identical to that given for the allele (in the database at http://viridans.eMLSA.net/ ) that is from S. salivarius strain sk729 (also described in reference 3); the other 5 targets in this MLST scheme for strain sk729 differed from those observed for 0735. ND, not determined.
↵ b These targets are as described in reference 6. Results with simple numbers indicate sequence identity to alleles with the same designation in this reference.
↵ c These targets are as described in reference 3 and at http://viridans.eMLSA.net/ .
↵ d Identical allelic and 16S rRNA gene sequences were obtained for brain isolate 0735, 4 additional CSF or blood isolates from the 2 case patients, and 12 carriage isolates recovered from the anesthesiologist.
↵ e Results for this isolate were obtained from an oral carriage strain highly related to 0735, taken from reference 6.
↵ f GenBank accession number for the 16S rRNA sequence found within reference strain SS908.