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Bacteriology

Genetic Diversity and Virulence Profiles of Escherichia coli Isolates Causing Spontaneous Bacterial Peritonitis and Bacteremia in Patients with Cirrhosis

Frédéric Bert, James R. Johnson, Bénédicte Ouattara, Véronique Leflon-Guibout, Brian Johnston, Estelle Marcon, Dominique Valla, Richard Moreau, Marie-Hélène Nicolas-Chanoine
Frédéric Bert
Service de Microbiologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, France
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  • For correspondence: frederic.bert@bjn.aphp.fr
James R. Johnson
Veterans Affairs Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota
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Bénédicte Ouattara
Service de Microbiologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, France
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Véronique Leflon-Guibout
Service de Microbiologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, France
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Brian Johnston
Veterans Affairs Medical Center and Department of Medicine, University of Minnesota, Minneapolis, Minnesota
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Estelle Marcon
Service de Microbiologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, France
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Dominique Valla
Service d'Hépatologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, FranceINSERM U 773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France
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Richard Moreau
Service d'Hépatologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, FranceINSERM U 773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France
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Marie-Hélène Nicolas-Chanoine
Service de Microbiologie, Hôpital Beaujon, Assistance Publique-Hôpitaux de Paris (AP-HP), Clichy, FranceINSERM U 773, Centre de Recherche Biomédicale Bichat-Beaujon CRB3, Paris, France
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DOI: 10.1128/JCM.00516-10
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ABSTRACT

Among patients with cirrhosis, infections caused by Escherichia coli organisms that translocate from the gut are a frequent and severe complication. One hundred ten E. coli isolates from 110 cirrhotic patients with spontaneous bacterial peritonitis and/or spontaneous bacteremia were characterized for their phylogenetic group and virulence genotype (34 extraintestinal virulence factor genes). Genetic relatedness was investigated by enterobacterial repetitive intergenic consensus sequence type 2 (ERIC-2) PCR typing and multilocus sequence typing. Phylogenetic groups A, B1, B2, and D accounted for 24%, 4%, 48%, and 24% of the population, respectively. Overall, 68 distinct ERIC-2 profiles were encountered. Eleven clonal groups, represented by multiple isolates (2 to 11) from the same sequence type (ST) or sequence type complex, were identified. These clonal groups accounted for 54 (49%) isolates overall. Membership in one of these clonal groups was more frequent among B2 isolates than non-B2 isolates (67% versus 32%, P < 0.001). The most frequent sequence types were ST95 (n = 13) and ST73 (n = 8), followed by the ST14 and ST10 complexes (n = 7). ST131 and ST69 were represented by three isolates each. Clonal group-associated isolates exhibited a greater prevalence of 11 virulence genes, including pap elements, than the other isolates. However, no association between clonal groups and host factors, type of infection, or mortality was observed. In conclusion, E. coli isolates causing spontaneous bacterial peritonitis and bacteremia in cirrhotic patients are genetically diverse. However, approximately half of the isolates belong to familiar clonal groups and exhibit extensive virulence profiles that may be associated with greater invasive potential.

FOOTNOTES

    • Received 11 March 2010.
    • Returned for modification 27 April 2010.
    • Accepted 21 May 2010.
  • ↵▿ Published ahead of print on 2 June 2010.

  • American Society for Microbiology
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Genetic Diversity and Virulence Profiles of Escherichia coli Isolates Causing Spontaneous Bacterial Peritonitis and Bacteremia in Patients with Cirrhosis
Frédéric Bert, James R. Johnson, Bénédicte Ouattara, Véronique Leflon-Guibout, Brian Johnston, Estelle Marcon, Dominique Valla, Richard Moreau, Marie-Hélène Nicolas-Chanoine
Journal of Clinical Microbiology Jul 2010, 48 (8) 2709-2714; DOI: 10.1128/JCM.00516-10

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Genetic Diversity and Virulence Profiles of Escherichia coli Isolates Causing Spontaneous Bacterial Peritonitis and Bacteremia in Patients with Cirrhosis
Frédéric Bert, James R. Johnson, Bénédicte Ouattara, Véronique Leflon-Guibout, Brian Johnston, Estelle Marcon, Dominique Valla, Richard Moreau, Marie-Hélène Nicolas-Chanoine
Journal of Clinical Microbiology Jul 2010, 48 (8) 2709-2714; DOI: 10.1128/JCM.00516-10
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