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Journal of Clinical Microbiology
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Virology

Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results

Recep Kesli, Hakkı Polat, Yuksel Terzi, Muhammet Guzel Kurtoglu, Yavuz Uyar
Recep Kesli
1Konya Education and Research Hospital, Department of Microbiology, Konya, Turkey
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  • For correspondence: recepkesli@yahoo.com
Hakkı Polat
2Selcuk University Meram Medical School, Department of Internal Diseases, Konya, Turkey
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Yuksel Terzi
3Ondokuzmayis University Faculty of Art and Science, Department of Statistics, Samsun, Turkey
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Muhammet Guzel Kurtoglu
1Konya Education and Research Hospital, Department of Microbiology, Konya, Turkey
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Yavuz Uyar
4Refik Saydam National Public Health Agency, Virology Reference Laboratory, Ankara, Turkey
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DOI: 10.1128/JCM.05292-11
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ABSTRACT

Hepatitis C virus (HCV) is a global health care problem. Diagnosis of HCV infection is mainly based on the detection of anti-HCV antibodies as a screening test with serum samples. Recombinant immunoblot assays are used as supplemental tests and for the final detection and quantification of HCV RNA in confirmatory tests. In this study, we aimed to compare the HCV core antigen test with the HCV RNA assay for confirming anti-HCV results to determine whether the HCV core antigen test may be used as an alternative confirmatory test to the HCV RNA test and to assess the diagnostic values of the total HCV core antigen test by determining the diagnostic specificity and sensitivity rates compared with the HCV RNA test. Sera from a total of 212 treatment-naive patients were analyzed for anti-HCV and HCV core antigen both with the Abbott Architect test and with the molecular HCV RNA assay consisting of a reverse transcription-PCR method as a confirmatory test. The diagnostic sensitivity, specificity, and positive and negative predictive values of the HCV core antigen assay compared to the HCV RNA test were 96.3%, 100%, 100%, and 89.7%, respectively. The levels of HCV core antigen showed a good correlation with those from the HCV RNA quantification (r = 0.907). In conclusion, the Architect HCV antigen assay is highly specific, sensitive, reliable, easy to perform, reproducible, cost-effective, and applicable as a screening, supplemental, and preconfirmatory test for anti-HCV assays used in laboratory procedures for the diagnosis of hepatitis C virus infection.

  • Copyright © 2011, American Society for Microbiology. All Rights Reserved.
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Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results
Recep Kesli, Hakkı Polat, Yuksel Terzi, Muhammet Guzel Kurtoglu, Yavuz Uyar
Journal of Clinical Microbiology Nov 2011, 49 (12) 4089-4093; DOI: 10.1128/JCM.05292-11

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Comparison of a Newly Developed Automated and Quantitative Hepatitis C Virus (HCV) Core Antigen Test with the HCV RNA Assay for Clinical Usefulness in Confirming Anti-HCV Results
Recep Kesli, Hakkı Polat, Yuksel Terzi, Muhammet Guzel Kurtoglu, Yavuz Uyar
Journal of Clinical Microbiology Nov 2011, 49 (12) 4089-4093; DOI: 10.1128/JCM.05292-11
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