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Bacteriology

Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for Clostridium difficile Detection Challenges Cytotoxin B Cell Test and Culture as Gold Standard

Torbjörn Norén, Ingegärd Alriksson, Josefin Andersson, Thomas Åkerlund, Magnus Unemo
Torbjörn Norén
1Department of Laboratory Medicine, Microbiology
2Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden
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  • For correspondence: torbjorn.noren@orebroll.se
Ingegärd Alriksson
1Department of Laboratory Medicine, Microbiology
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Josefin Andersson
1Department of Laboratory Medicine, Microbiology
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Thomas Åkerlund
3Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden
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Magnus Unemo
1Department of Laboratory Medicine, Microbiology
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DOI: 10.1128/JCM.01824-10
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  • Table 1.

    The novel Clostridium difficile diagnostic assay loop-mediated isothermal amplification (LAMP; Illumigene) compared to cell culture cytotoxin B (CTBA) and/or toxigenic culture (TC) as the gold standarda

    Diagnostic assayDiagnostic assay compared to CTBA plus TC
    SensitivitySpecificityPPVNPV
    % (no. of specimens/total no.)CI (%)% (no. of specimens/total no.)CI (%)% (no. of specimens/total no.)CI (%)% (no. of specimens/total no.)CI (%)
    LAMP98 (49/50)89–10098 (218/222)95–9992 (49/53)82–9899 (218/219)97–100
    CTBA72 (36/50)57–84100 (222/222)98–100100 (36/36)90–10094 (222/236)90–97
    TC100 (50/50)93–100100 (222/222)98–100100 (50/50)93–100100 (222/222)98–100
    • ↵a One of the four specimens that tested positive by LAMP only was positive in an in-house rpoA PCR, indicating that both the sensitivity and specificity of the LAMP test are higher. PPV, positive predictive value; NPV, negative predictive value; CI, confidence interval.

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Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for Clostridium difficile Detection Challenges Cytotoxin B Cell Test and Culture as Gold Standard
Torbjörn Norén, Ingegärd Alriksson, Josefin Andersson, Thomas Åkerlund, Magnus Unemo
Journal of Clinical Microbiology Feb 2011, 49 (2) 710-711; DOI: 10.1128/JCM.01824-10

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Rapid and Sensitive Loop-Mediated Isothermal Amplification Test for Clostridium difficile Detection Challenges Cytotoxin B Cell Test and Culture as Gold Standard
Torbjörn Norén, Ingegärd Alriksson, Josefin Andersson, Thomas Åkerlund, Magnus Unemo
Journal of Clinical Microbiology Feb 2011, 49 (2) 710-711; DOI: 10.1128/JCM.01824-10
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