Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotherapy
    • Applied and Environmental Microbiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • COVID-19 Special Collection
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Virology

Standardization of Hepatitis E Virus (HEV) Nucleic Acid Amplification Technique-Based Assays: an Initial Study To Evaluate a Panel of HEV Strains and Investigate Laboratory Performance

Sally A. Baylis, Kay-Martin Hanschmann, Johannes Blümel, C. Micha Nübling; on behalf of the HEV Collaborative Study Group
Sally A. Baylis
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: baysa@pei.de
Kay-Martin Hanschmann
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Johannes Blümel
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
C. Micha Nübling
Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JCM.02578-10
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Additional Files
  • Fig. 1.
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Phylogenetic analysis of HEV strains evaluated in the study. GenBank accession numbers are shown for reference strains; the numbers of the panel strains are shown in Table 1.

  • Fig. 2.
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Analysis of viral loads (log10 copies/ml) by laboratory and sample. (a) HRC-HE104 genotype 3a. (b) JRC-HE3 genotype 3b. (c) RKI genotype 3f. (d) HRC-HE15 genotype 4c.

Tables

  • Figures
  • Additional Files
  • Table 1.

    Details of HEV strains evaluated in the study

    GenotypeVirus strainHEV RNA level (copies/ml)aGenBank accession no.Anti-HEV IgM/IgG resultbALT level (IU/liter)c
    3aHRC-HE1041.6 × 107AB602891−/−36
    3bJRC-HE32.5 × 107AB434146+/−398
    3fRKI1.3 × 106FJ956757−/−Negative
    4cHRC-HE151.0 × 106AB602890−/−505
    • ↵a Concentrations determined by laboratories where samples were identified.

    • ↵b +/+, positive result; +/−, equivocal result; −/−, negative result.

    • ↵c ALT, alanine transaminase.

  • Table 2.

    Qualitative analysis of the four HEV strains

    Virus strain (genotype)Nominal concn (log10 copies/ml)% positivea
    HRC-HE104 (3a)6.296
    5.296
    4.292/88
    3.275/67
    2.238/25
    1.213/8
    JRC-HE3 (3b)6.496
    5.492
    4.492/88
    3.475/67
    2.458/33
    1.421/9
    RKI (3f)5.192
    4.175/71
    3.171/63
    2.133/25
    1.14
    HRC-HE15 (4c)5.092/88
    4.083
    3.050/38
    2.033/25
    1.0b4/0
    • ↵a For the number of positive test results, a best-case/worst-case percentage is reported for results reported as being simply positive or equivocal.

    • ↵b Data were returned for a total number of 24 tests for each sample. However, in the case of sample 22 (genotype 4c), a false-positive result (as evidenced by the detection of an HEV genotype not represented in the panel) was reported by one laboratory and was not included during the calculation of percent positive results. A breakdown of the original data is shown in Tables S2 to S5 in the supplemental material.

  • Table 3.

    Viral load data for the four HEV strains

    Virus strainSampleNominal concn (log10 copies/ml)No. of samplesObserved viral load (log10 copies/ml)
    Geometric meanSD95% CIaMedianMinimumMaximum
    HRC-HE104136.2125.840.645.43, 6.245.774.827.48
    115.2124.740.684.31, 5.174.723.636.40
    34.2113.850.673.40, 4.303.843.115.64
    183.293.040.662.53, 3.542.962.404.49
    172.261.980.371.59, 2.371.941.432.40
    191.240.101.27−1.93, 2.120.00−1.001.38
    JRC-HE3206.4126.160.725.70, 6.616.154.437.70
    235.4125.071.094.38, 5.765.142.157.00
    44.4124.210.673.79, 4.634.272.605.58
    123.4103.400.602.97, 3.833.202.925.00
    102.462.431.031.35, 3.522.361.264.11
    61.431.402.39−4.53, 7.341.43−1.003.78
    RKI15.1124.630.594.26, 5.014.573.916.26
    84.1103.770.613.33, 4.213.633.205.26
    23.192.830.692.30, 3.372.631.774.28
    162.161.681.540.06, 3.301.80−1.003.28
    211.13−0.321.18−3.25, 2.61−1.00−1.001.04
    HRC-HE1555.0124.560.694.12, 5.004.443.286.28
    144.0103.400.383.13, 3.673.442.634.04
    93.081.831.850.28, 3.382.46−1.004.20
    152.061.021.83−0.90, 2.941.35−1.003.85
    221.03−1.000.00−1.00−1.00−1.00
    • ↵a 95% CI, 95% confidence interval for the geometric mean.

Additional Files

  • Figures
  • Tables
  • Supplemental material

    Files in this Data Supplement:

    • Supplemental file 1 - Tables S1 (Assay protocols used by participants), S2 (Qualitative analysis of HRC-HE104 genotype 3a dilutions), S3 (Qualitative analysis of JRC-HE3 genotype 3b dilutions), S4 (Qualitative analysis of RKI genotype 3f dilutions), and S5 (Qualitative analysis of HRC-HE15 genotype 4c dilutions)
      Zipped MS Word document, 32K.
PreviousNext
Back to top
Download PDF
Citation Tools
Standardization of Hepatitis E Virus (HEV) Nucleic Acid Amplification Technique-Based Assays: an Initial Study To Evaluate a Panel of HEV Strains and Investigate Laboratory Performance
Sally A. Baylis, Kay-Martin Hanschmann, Johannes Blümel, C. Micha Nübling on behalf of the HEV Collaborative Study Group
Journal of Clinical Microbiology Apr 2011, 49 (4) 1234-1239; DOI: 10.1128/JCM.02578-10

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Standardization of Hepatitis E Virus (HEV) Nucleic Acid Amplification Technique-Based Assays: an Initial Study To Evaluate a Panel of HEV Strains and Investigate Laboratory Performance
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Standardization of Hepatitis E Virus (HEV) Nucleic Acid Amplification Technique-Based Assays: an Initial Study To Evaluate a Panel of HEV Strains and Investigate Laboratory Performance
Sally A. Baylis, Kay-Martin Hanschmann, Johannes Blümel, C. Micha Nübling on behalf of the HEV Collaborative Study Group
Journal of Clinical Microbiology Apr 2011, 49 (4) 1234-1239; DOI: 10.1128/JCM.02578-10
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENT
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

 

American Society for Microbiology
1752 N St. NW
Washington, DC 20036
Phone: (202) 737-3600

 

Copyright © 2021 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X