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Mycobacteriology and Aerobic Actinomycetes

Isolation of Mycobacterium ulcerans from Swab and Fine-Needle-Aspiration Specimens

Dorothy Yeboah-Manu, Emelia Danso, Kobina Ampah, Adwoa Asante-Poku, Zuliehatu Nakobu, Gerd Pluschke
Dorothy Yeboah-Manu
1Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
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  • For correspondence: dyeboah-manu@noguchi.mimcom.org
Emelia Danso
1Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
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Kobina Ampah
1Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
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Adwoa Asante-Poku
1Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
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Zuliehatu Nakobu
1Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana
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Gerd Pluschke
2Swiss Tropical and Public Health Institute, Basel, Switzerland
3University of Basel, Basel, Switzerland
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DOI: 10.1128/JCM.02279-10
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    Fig. 1.

    Laboratory diagnosis of preulcerative Buruli ulcer lesion by Ziehl-Neelsen staining of a smear prepared from an FNA sample. The black arrows point to acid-fast bacilli.

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    Result obtained with the different decontamination procedures that were evaluated in this studya

    Decontamination methodNo. (%) of cultures
    Culture positive (n = 20)No bacterial growth (n = 20)Contaminated (n = 20)
    Oxalic acid method10 (50)9 (45)1 (5)
    Petroff method10 (50)8 (40)2 (10)
    Double decontamination1 (5)19 (95)0 (0)
    • ↵a A culture was considered positive if at least one tube gave an isolate that was confirmed as M. ulcerans and contaminated if all tubes inoculated were overgrown by other bacteria.

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Isolation of Mycobacterium ulcerans from Swab and Fine-Needle-Aspiration Specimens
Dorothy Yeboah-Manu, Emelia Danso, Kobina Ampah, Adwoa Asante-Poku, Zuliehatu Nakobu, Gerd Pluschke
Journal of Clinical Microbiology Apr 2011, 49 (5) 1997-1999; DOI: 10.1128/JCM.02279-10

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Isolation of Mycobacterium ulcerans from Swab and Fine-Needle-Aspiration Specimens
Dorothy Yeboah-Manu, Emelia Danso, Kobina Ampah, Adwoa Asante-Poku, Zuliehatu Nakobu, Gerd Pluschke
Journal of Clinical Microbiology Apr 2011, 49 (5) 1997-1999; DOI: 10.1128/JCM.02279-10
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