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Bacteriology

Comparison of Direct Colony Method versus Extraction Method for Identification of Gram-Positive Cocci by Use of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Adnan A. Alatoom, Scott A. Cunningham, Sherry M. Ihde, Jayawant Mandrekar, Robin Patel
Adnan A. Alatoom
1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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Scott A. Cunningham
1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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Sherry M. Ihde
1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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Jayawant Mandrekar
2Division of Biomedical Statistics and Informatics, Department of Health Sciences and Research
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Robin Patel
1Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology
3Division of Infectious Disease, Department of Medicine, Mayo Clinic, Rochester, Minnesota 55905
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  • For correspondence: patel.robin@mayo.edu
DOI: 10.1128/JCM.00506-11
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    Fig. 1.

    Percent identification of staphylococci, streptococci, enterococci, and “related genera” to the genus and species levels by the Bruker Biotyper system.

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  • Table 1.

    Bruker Biotyper identification of staphylococci, streptococci, and related genera to the genus and species levels by use of direct colony testing and preparatory extraction

    OrganismNo. of isolatesNo. of isolates (% correct) identified by:No. of spectra in database
    ExtractionDirect colony
    Genus levelSpecies levelGenus levelSpecies level
    Staphylococci, streptococci, and enterococci
        Staphylococcus aureus20201918812
        Staphylococcus lugdunensis888715
        Staphylococcus epidermidis1414111119
        Staphylococcus saprophyticus630008
        Staphylococcus capitis666426
        Staphylococcus haemolyticus555208
        Staphylococcus hominis554426
        Staphylococcus pettenkoferi550104
        Staphylococcus schleiferi444006
        Staphylococcus intermedius/S. pseudointermedius221003
        Staphylococcus simulans220005
        Staphylococcus pasteuri/S. warneri111102
        Streptococcus pyogenes101010418
        Streptococcus agalactiae7771110
        Group C beta-hemolytic streptococci66611NA
        Group F beta-hemolytic streptococci11110NA
        Group G beta-hemolytic streptococci22100NA
        Streptococcus pneumoniae101010639
        Streptococcus mitis group1010310210
        Streptococcus mutans group101089610
        Streptococcus salivarius group11117606
        Streptococcus anginosus group11105945
        Streptococcus bovis group1313109211
        Streptococcus dysgalactiae111003
        Streptococcus equi subsp. zooepidemicus111105
        Streptococcus halichoeri111001
        Enterococcus faecium15151514129
        Enterococcus faecalis1515151178
        Enterococcus avium666102
        Enterococcus raffinosus220001
        Enterococcus avium/E. raffinosus111003
        Enterococcus gallinarum111113
        Enterococcus casseliflavus/E. gallinarum442428
        Enterococcus gilvus111001
        Total217213 (98)171 (79)136 (63)56 (26)
    Related genera
        Rothia mucilaginosa11111103
        Rothia dentocariosa/R. aeria654205
        Micrococcus luteus/M. yunnanensis665402
        Micrococcus luteus321112
        Granulicatella adiacens750001
        Granulicatella elegans100001
        Abiotrophia defectiva666502
        Aerococcus sanguinicola12114402
        Aerococcus urinae775303
        Aerococcus viridans110005
        Gemella morbillorum200001
        Gemella sp.220202
        Kocuria kristinae331003
        Kocuria rosea111105
        Kocuria palustris110001
        Helcococcus kunzii222202
        Lactococcus lactis444425
        Leuconostoc citreum111112
        Pediococcus sp.111102
        Facklamia languida110101
        Facklamia ignava100001
        Arthrobacter cumminsii110001
        Macrococcus caseolyticus100001
        Total8171 (88)36 (44)32 (40)4 (5)
  • Table 2.

    Numbers and percentages of isolates identified with lowered scores assigned for genus and species identifications using extraction and direct colony methods

    Score cutoffNo. (%) of isolates identified
    ExtractionDirect colony
    Genus
        ≥1.4295 (99)226 (76)
        ≥1.5293 (98)214 (72)
        ≥1.6291 (98)191 (64)
        ≥1.7284 (95)168 (56)
    Species
        ≥1.7276 (93)161 (54)
        ≥1.8248 (83)129 (43)
        ≥1.9224 (75)95 (32)
        ≥2.0207 (69)60 (20)
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Comparison of Direct Colony Method versus Extraction Method for Identification of Gram-Positive Cocci by Use of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
Adnan A. Alatoom, Scott A. Cunningham, Sherry M. Ihde, Jayawant Mandrekar, Robin Patel
Journal of Clinical Microbiology Jul 2011, 49 (8) 2868-2873; DOI: 10.1128/JCM.00506-11

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Comparison of Direct Colony Method versus Extraction Method for Identification of Gram-Positive Cocci by Use of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
Adnan A. Alatoom, Scott A. Cunningham, Sherry M. Ihde, Jayawant Mandrekar, Robin Patel
Journal of Clinical Microbiology Jul 2011, 49 (8) 2868-2873; DOI: 10.1128/JCM.00506-11
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