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Journal of Clinical Microbiology
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Bacteriology

Ultrarapid Detection of bla KPC1/2-12 from Perirectal and Nasal Swabs by Use of Real-Time PCR

Sara N. Richter, Ilaria Frasson, Maria Angela Biasolo, Andrea Bartolini, Antonietta Cavallaro, Giorgio Palù
Sara N. Richter
aDepartment of Molecular Medicine, Padua, Italy
bAzienda Ospedaliera di Padova, Microbiology and Virology Unit, Padua, Italy
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Ilaria Frasson
aDepartment of Molecular Medicine, Padua, Italy
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Maria Angela Biasolo
aDepartment of Molecular Medicine, Padua, Italy
bAzienda Ospedaliera di Padova, Microbiology and Virology Unit, Padua, Italy
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Andrea Bartolini
bAzienda Ospedaliera di Padova, Microbiology and Virology Unit, Padua, Italy
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Antonietta Cavallaro
bAzienda Ospedaliera di Padova, Microbiology and Virology Unit, Padua, Italy
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Giorgio Palù
aDepartment of Molecular Medicine, Padua, Italy
bAzienda Ospedaliera di Padova, Microbiology and Virology Unit, Padua, Italy
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DOI: 10.1128/JCM.00195-12
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ABSTRACT

The novel real-time PCR assay developed as described here was able to detect bla KPC1/2-12 (bla KPC-1/2 to bla KPC-12) from easily available clinical specimens in less than 2 h. The genotypic assay was highly sensitive (100%) and specific (98%). In some cases, it was able to detect bla KPC 48 h before positive detection by standard phenotypic assay on patients who were monitored daily. The high sensitivity and rapidity of the molecular method make it the method of choice for KPC surveillance and, thus, containment purposes.

  • Copyright © 2012, American Society for Microbiology. All Rights Reserved.
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Ultrarapid Detection of bla KPC1/2-12 from Perirectal and Nasal Swabs by Use of Real-Time PCR
Sara N. Richter, Ilaria Frasson, Maria Angela Biasolo, Andrea Bartolini, Antonietta Cavallaro, Giorgio Palù
Journal of Clinical Microbiology Apr 2012, 50 (5) 1718-1720; DOI: 10.1128/JCM.00195-12

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Ultrarapid Detection of bla KPC1/2-12 from Perirectal and Nasal Swabs by Use of Real-Time PCR
Sara N. Richter, Ilaria Frasson, Maria Angela Biasolo, Andrea Bartolini, Antonietta Cavallaro, Giorgio Palù
Journal of Clinical Microbiology Apr 2012, 50 (5) 1718-1720; DOI: 10.1128/JCM.00195-12
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