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Bacteriology

wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains

Sylvain Brisse, Virginie Passet, Anita Björk Haugaard, Anamaria Babosan, Najiby Kassis-Chikhani, Carsten Struve, Dominique Decré
Sylvain Brisse
aInstitut Pasteur, Microbial Evolutionary Genomics, Paris, France
bCNRS, UMR3525, Paris, France
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Virginie Passet
aInstitut Pasteur, Microbial Evolutionary Genomics, Paris, France
bCNRS, UMR3525, Paris, France
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Anita Björk Haugaard
cDepartment of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark
dWHO Collaborating Centre for Reference and Research on and , Statens Serum Institut, Copenhagen, Denmark
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Anamaria Babosan
eDépartement de Bactériologie, Université Pierre et Marie Curie, Faculté de Médecine, and Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Est Parisiens, Paris, France
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Najiby Kassis-Chikhani
fLaboratoire de Bactériologie, Hôpital Paul Brousse, Assistance Publique-Hôpitaux de Paris, Villejuif, France
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Carsten Struve
cDepartment of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark
dWHO Collaborating Centre for Reference and Research on and , Statens Serum Institut, Copenhagen, Denmark
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Dominique Decré
eDépartement de Bactériologie, Université Pierre et Marie Curie, Faculté de Médecine, and Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Est Parisiens, Paris, France
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DOI: 10.1128/JCM.01924-13
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    Fig 1

    Dendrogram of 135 wzi alleles. The tree was built using BioNumerics version 6.6 based on the uncorrected p distance. Each sequence corresponds to a distinct wzi allele, as indicated. The allele number is followed by the corresponding capsular type(s). Dots separating several K types indicate cross-reactions, whereas successions of distinct K types with no dot (e.g., K15K17K50K51K52) indicate that the wzi allele was found in strains reacting individually with one of these distinct K types. The three main branches (A, B, and C) are labeled. K P-2008 indicates the novel serotype described by Pan et al. (17). Bootstrap values of the remarkable nodes are given.

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    • Supplemental file 1 -

      Fig. S1 (Phylogenetic relationships among the 77 reference strains of the international Klebsiella serotyping scheme, based on rpoB gene sequencing)

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    • Supplemental file 2 -

      Table S1 (Correspondence of wzi alleles with K types)

      PDF, 10.0K

    • Supplemental file 3 -

      Sequences of the 135 distinct wzi alleles found in this study

      PDF, 75K

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wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains
Sylvain Brisse, Virginie Passet, Anita Björk Haugaard, Anamaria Babosan, Najiby Kassis-Chikhani, Carsten Struve, Dominique Decré
Journal of Clinical Microbiology Nov 2013, 51 (12) 4073-4078; DOI: 10.1128/JCM.01924-13

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wzi Gene Sequencing, a Rapid Method for Determination of Capsular Type for Klebsiella Strains
Sylvain Brisse, Virginie Passet, Anita Björk Haugaard, Anamaria Babosan, Najiby Kassis-Chikhani, Carsten Struve, Dominique Decré
Journal of Clinical Microbiology Nov 2013, 51 (12) 4073-4078; DOI: 10.1128/JCM.01924-13
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