DOI: 10.1128/JCM.02478-12
Article Figures & Data
Figures
- Fig 1
Real-time PCR assay simultaneously detects nine different DEC virulence genes. Data from individual reaction mixtures, each containing DNA from ETEC, EAEC, STEC, EPEC, EIEC, or DAEC strains, are presented in a single graph to show the separation between individual amplicon melting curves.
Tables
- Table 1
Primers used for real-time multiplex PCR
Target gene and primer name Primer sequence (5′–3′) Concn (μM) Source or reference eae EAE-S for ACT GGA CTT CTT ATT RCC GTT CTA TG 0.2 8 EAE-B2 rev CCT AAA CGG GTA TTA TCA CCA GA pEAF EP-1 for GTT CTT GGC GAA CAG GCT TGT C 0.2 8 EP-2 rev TTA AGC CAG CTA CCA TCC ACC C aatA EA-1 for AGG TTT GAT ATT GAT GTC CTT GAG GA 0.1 8 EA-2 rev TCA GCT AAT AAT GTA TAG AAA TCC GCT GTT daaC DAA-F ATT ACG TCA TCC GGG AAG CAC ACA 0.1 This study DAA-R GCT TGC TCA TAA AGC CGC AGA CAA elt LTf GGC GAC AGA TTA TAC CGT GC 0.2 11 LTr CGG TCT CTA TAT TCC CTG TT est STa-F ATT TTT MTT TCT GTA TTR TCT T 0.4 11 STa-R CAC CCG GTA CAR GCA GGA TT ipaH IpaH1 GTT CCT TGA CCG CCT TTC CGA TAC CGT C 0.2 10 IpaH2 GCC GGT CAG CCA CCC TCT GAG AGT AC stx1 stxA1 598 AGT CGT ACG GGG ATG CAG ATA AAT 0.1 7 stxA1 1015 CCG GAC ACA TAG AAG GAA ACT CAT stx2 Stx2f GGC ACT GTC TGA AAC TGC CC 0.1 7 Stx2r TCG CCA GTT ATC TGA CAT TCT G
