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Bacteriology

Analytical Evaluation of GeneXpert CT/NG, the First Genetic Point-of-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis

Sepehr N. Tabrizi, Magnus Unemo, Daniel Golparian, Jimmy Twin, Athena E. Limnios, Monica Lahra, Rebecca Guy
Sepehr N. Tabrizi
aDepartment of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia
bDepartment of Obstetrics and Gynaecology, University of Melbourne, The Royal Women's Hospital and Murdoch Children's Research Institute, Parkville, Victoria, Australia
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Magnus Unemo
cWHO Collaborating Centre for Gonorrhoea and other STIs, Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden
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Daniel Golparian
cWHO Collaborating Centre for Gonorrhoea and other STIs, Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden
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Jimmy Twin
bDepartment of Obstetrics and Gynaecology, University of Melbourne, The Royal Women's Hospital and Murdoch Children's Research Institute, Parkville, Victoria, Australia
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Athena E. Limnios
dWHO Collaborating Centre for STD, Microbiology Department, The Prince of Wales Hospital, Randwick, Sydney, Australia
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Monica Lahra
dWHO Collaborating Centre for STD, Microbiology Department, The Prince of Wales Hospital, Randwick, Sydney, Australia
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Rebecca Guy
eDepartment of Microbiology and the Kirby Institute, University of NSW, Sydney, Australia
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DOI: 10.1128/JCM.00806-13
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ABSTRACT

GeneXpert CT/NG was evaluated with 372 characterized bacterial strains. Sensitivity of 10 genome copies/reaction was obtained for both agents. Four Neisseria mucosa and two Neisseria subflava isolates were positive for one of two gonococcal targets; however, the assay flagged all as negative. The assay was analytically highly sensitive and specific.

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Analytical Evaluation of GeneXpert CT/NG, the First Genetic Point-of-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis
Sepehr N. Tabrizi, Magnus Unemo, Daniel Golparian, Jimmy Twin, Athena E. Limnios, Monica Lahra, Rebecca Guy on behalf of the TTANGO Investigators
Journal of Clinical Microbiology May 2013, 51 (6) 1945-1947; DOI: 10.1128/JCM.00806-13

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Analytical Evaluation of GeneXpert CT/NG, the First Genetic Point-of-Care Assay for Simultaneous Detection of Neisseria gonorrhoeae and Chlamydia trachomatis
Sepehr N. Tabrizi, Magnus Unemo, Daniel Golparian, Jimmy Twin, Athena E. Limnios, Monica Lahra, Rebecca Guy on behalf of the TTANGO Investigators
Journal of Clinical Microbiology May 2013, 51 (6) 1945-1947; DOI: 10.1128/JCM.00806-13
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