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Journal of Clinical Microbiology
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Bacteriology

PCR-Electrospray Ionization Mass Spectrometry for Direct Detection of Pathogens and Antimicrobial Resistance from Heart Valves in Patients with Infective Endocarditis

Cassandra L. Brinkman, Paschalis Vergidis, James R. Uhl, Bobbi S. Pritt, Franklin R. Cockerill, James M. Steckelberg, Larry M. Baddour, Joseph J. Maleszewski, William D. Edwards, Rangarajan Sampath, Robin Patel
Cassandra L. Brinkman
aDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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Paschalis Vergidis
aDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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James R. Uhl
aDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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Bobbi S. Pritt
aDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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Franklin R. Cockerill
aDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology
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James M. Steckelberg
bDivision of Infectious Diseases, Department of Internal Medicine
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Larry M. Baddour
bDivision of Infectious Diseases, Department of Internal Medicine
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Joseph J. Maleszewski
cDivision of Anatomic Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA
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William D. Edwards
cDivision of Anatomic Pathology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA
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Rangarajan Sampath
dIbis Biosciences, Abbott, Carlsbad, California, USA
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Robin Patel
aDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology
bDivision of Infectious Diseases, Department of Internal Medicine
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DOI: 10.1128/JCM.00304-13
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ABSTRACT

Microbiological diagnosis is pivotal to the appropriate management and treatment of infective endocarditis. We evaluated PCR-electrospray ionization mass spectrometry (PCR/ESI-MS) for bacterial and candidal detection using 83 formalin-fixed paraffin-embedded heart valves from subjects with endocarditis who had positive valve and/or blood cultures, 63 of whom had positive valvular Gram stains. PCR/ESI-MS yielded 55% positivity with concordant microbiology at the genus/species or organism group level (e.g., viridans group streptococci), 11% positivity with discordant microbiology, and 34% with no detection. PCR/ESI-MS detected all antimicrobial resistance encoded by mecA or vanA/B and identified a case of Tropheryma whipplei endocarditis not previously recognized.

  • Copyright © 2013, American Society for Microbiology. All Rights Reserved.
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PCR-Electrospray Ionization Mass Spectrometry for Direct Detection of Pathogens and Antimicrobial Resistance from Heart Valves in Patients with Infective Endocarditis
Cassandra L. Brinkman, Paschalis Vergidis, James R. Uhl, Bobbi S. Pritt, Franklin R. Cockerill, James M. Steckelberg, Larry M. Baddour, Joseph J. Maleszewski, William D. Edwards, Rangarajan Sampath, Robin Patel
Journal of Clinical Microbiology Jun 2013, 51 (7) 2040-2046; DOI: 10.1128/JCM.00304-13

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PCR-Electrospray Ionization Mass Spectrometry for Direct Detection of Pathogens and Antimicrobial Resistance from Heart Valves in Patients with Infective Endocarditis
Cassandra L. Brinkman, Paschalis Vergidis, James R. Uhl, Bobbi S. Pritt, Franklin R. Cockerill, James M. Steckelberg, Larry M. Baddour, Joseph J. Maleszewski, William D. Edwards, Rangarajan Sampath, Robin Patel
Journal of Clinical Microbiology Jun 2013, 51 (7) 2040-2046; DOI: 10.1128/JCM.00304-13
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