Article Figures & Data
Figures
- Fig 1
Flow chart describing our large-volume blood sample-processing methodology.
- Fig 2
Effect of blood volume on the probability of mycobacterium detection. Various numbers of BCG CFU per milliliter were spiked into different volumes of blood. The percentages of samples among seven samples per concentration that were positive at each concentration are shown.
- Fig 3
Comparison of various anticoagulants. Blood samples anticoagulated with ACD-A, ACD-B, or EDTA were spiked with 5 and 10 CFU/ml of BCG in 5 ml whole blood. The proportion of samples that were positive (bars) and the CT value of the rpoB assay (■) are shown for each condition. The number of replicates run with each anticoagulant type was 4 to 7.
- Fig 4
LOD of mycobacteria in ACD-B-anticoagulated blood. BCG was spiked into 5-ml blood aliquots. The proportion of positive samples for each CFU concentration out of 15 samples tested per concentration is shown. Each replicate is indicative of a blood sample from one healthy individual.
- Fig 5
Solution and sample stability. Stability studies were performed by running Xpert MTB/RIF assays with lysis solution stored for 0 to 90 days at RT (♢) or under refrigerated conditions (4 to 8°C) (■) (A) and BCG-spiked blood samples stored for 0 to 8 days (B). Each time point shows the mean of five test results. An increase in the CT (not shown) would have indicated that a stability storage limit had been reached.
