Article Figures & Data
Figures
- FIG 1
PFGE of salivary and pulmonary P. aeruginosa isolates. Analysis of the genetic relationship with the XbaI restriction enzyme was performed with the 5 CC patients. Seven clonal groups were found. The genetic profile of the reference P. aeruginosa PAO1 strain is also shown.
Tables
- TABLE 1
Demographic data of the CF CC and NC patients
Parameter Value(s) for indicated patient group CC NC Total no. of patients 5 5 No. of females 2 2 No. of males 3 3 Avg age (yrs) 23.8 16.6 Age range (yrs) 16–34 12–27 - TABLE 2
Demographic and clinical data of the CF patientsa
Patient Age (yrs) Sex Oral conditions No. of DMFT No. of cells/field Sputum quality No. of isolates of P. aeruginosa PFGE clonal group(s) SECs WBCs Saliva Sputum Saliva Sputum Wild type Mucoid Wild type Mucoid Wild type Mucoid Wild type Mucoid NC 1 12 M Ng&Np 0 >25 <10 Poor 0 0 0 0 NC 2 13 M Ng&Np 0 10–25 >25 Acceptable 0 0 0 0 NC 3 15 F Ng&Np 1 >25 <10 Poor 0 0 0 0 NC 4 16 M Ng&Np 4 <10 <25 Appropriate 0 0 0 0 NC 5 27 F Ng&Np 12 <10 >25 Appropriate 0 0 0 0 CC 1 16 F Ng&Np 1 10–25 >25 Acceptable 2 0 1 0 5 and 6 5 CC 2 17 M Ng&Np 1 10–25 >25 Acceptable 1 0 1 0 1 1 CC 3 24 M Ng&Np 4 >25 10–25 Poor 0 0 1 1 7a 7b CC 4 28 M Ng&Np 9 10–25 >25 Acceptable 2 1 0 2 4 4 4 CC 5 34 F Ng&Np 6 10–25 >25 Acceptable 0 0 2 2 2 and 3 2 and 3 ↵a NC, patients not colonized by P. aeruginosa; CC, patients chronically colonized by P. aeruginosa; M, male; F, female; Ng&Np, no gingivitis and no periodontitis; DFMT, decayed, missing, or filled teeth; SECs, squamous epithelial cells; WBCs, white blood cells.
- TABLE 3
qPCR of subgingival plaque, saliva, and sputum samples in the CF patientsa
Patient Result of oprL qPCR Result of qPCR for gyrB and ecfX P. aeruginosa qPCR quantification (CFU/ml) Subgingival plaque Saliva Sputum Subgingival plaque Saliva Sputum Subgingival plaque Saliva Sputum NC 1 − − + NM NM − 0 0 0 NC 2 − − + NM NM − 0 0 0 NC 3 − − − NM NM NM 0 0 0 NC 4 − − + NM NM − 0 0 0 NC 5 − − + NM NM − 0 0 0 CC 1 − + + − + + 0 4.2 · 103 2.6 · 106 CC 2 + + + + + + 1.0 · 103 4.8 · 105 4.5 · 109 CC 3 + + + ± − + 1.2 · 101 0 1.1 · 107 CC 4 + + + + + + 1.12 · 106 2.1 · 107 1.7 · 108 CC 5 + + + − − + 0 0 1.2 · 109 ↵a NC, patients not colonized by P. aeruginosa; CC, patients chronically colonized by P. aeruginosa; −, negative detection result; +, positive detection result; ±, positive for gyrB and negative for ecfX; NM, determination not made.
- TABLE 4
Anaerobic species detection and quantification by qPCR in subgingival plaque and sputum samplesa
Species Sample qPCR quantification (CFU/ml) in sample from indicated patient NC 1 NC 2 NC 3 NC 4 NC 5 CC 1 CC 2 CC 3 CC 4 CC 5 Treponema denticola Subgingival plaque 0 0 0 0 3.83 · 104 0 0 0 0 0 Sputum 0 0 0 0 0 0 0 0 0 0 Tannerella forsythia Subgingival plaque 0 0 5.78 · 103 0 0 0 0 0 0 0 Sputum 0 0 0 0 0 0 0 0 0 0 Porphyromonas gingivalis Subgingival plaque 0 0 0 0 0 0 0 0 6.75 · 103 0 Sputum 0 0 0 0 0 0 0 0 0 0 Campylobacter rectus Subgingival plaque 5.73 · 105 0 8.70 · 103 0 9.38 · 105 0 0 0 0 0 Sputum 4.07 · 104 0 0 0 3.95 · 103 0 0 0 0 0 Fusobacterium nucleatum Subgingival plaque 3.70 · 106 4.24 · 104 2.38 · 105 2.08 · 104 5.82 · 106 1.25 · 107 3.79 · 105 7.58 · 104 2.77 · 106 0 Sputum 3.75 · 105 4.37 · 104 0 2.66 · 104 0 4.12 · 104 0 0 1.53 · 104 0 Parvimonas micra Subgingival plaque 1.41 · 104 0 9.68 · 103 0 5.34 · 105 0 0 0 4.90 · 105 0 Sputum 3.16 · 103 0 0 2.14 · 102 1.20 · 102 0 0 0 6.45 · 102 0 Prevotella intermedia Subgingival plaque 1.54 · 105 0 1.92 · 104 0 0 0 0 9.90 · 104 1.64 · 106 0 Sputum 2.61 · 106 0 0 0 0 0 0 0 0 0 Eikenella corrodens Subgingival plaque 4.59 · 106 5.35 · 104 1.42 · 104 9.22 · 103 4.37 · 105 1.77 · 106 5.77 · 104 0 0 0 Sputum 2.07 · 105 2.06 · 104 5.10 · 104 3.81 · 105 0 4.62 · 104 1.31 · 105 0 0 0 Aggregatibacter actinomycetemcomitans Subgingival plaque 0 0 0 0 0 0 0 0 0 0 Sputum 0 0 0 0 0 0 0 0 0 0 Total flora Subgingival plaque 7.13 · 109 2.17 · 108 7.80 · 108 5.48 · 108 8.18 · 109 2.70 · 109 2.80 · 108 4.07 · 109 4.18 · 109 1.71 · 108 Sputum 5.20 · 108 1.31 · 108 4.26 · 108 4.90 · 108 5.35 · 108 1.57 · 108 8.45 · 108 7.20 · 107 9.60 · 107 3.93 · 109 ↵a NC, patients not colonized by P. aeruginosa; CC, patients chronically colonized by P. aeruginosa.
