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Journal of Clinical Microbiology
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Bacteriology

Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing

James Heng Chiak Sim, Victoria Anikst, Akshar Lohith, Nader Pourmand, Niaz Banaei
N. A. Ledeboer, Editor
James Heng Chiak Sim
aDepartment of Pathology, Stanford University, Stanford, California, USA
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Victoria Anikst
aDepartment of Pathology, Stanford University, Stanford, California, USA
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Akshar Lohith
bDepartment of Biomolecular Engineering, University of California, Santa Cruz, California, USA
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Nader Pourmand
bDepartment of Biomolecular Engineering, University of California, Santa Cruz, California, USA
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Niaz Banaei
aDepartment of Pathology, Stanford University, Stanford, California, USA
cDepartment of Medicine, Stanford University, Stanford, California, USA
dClinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA
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N. A. Ledeboer
Roles: Editor
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DOI: 10.1128/JCM.00956-15
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Article Information

DOI 
https://doi.org/10.1128/JCM.00956-15
PubMed 
25878343

Published By 
American Society for Microbiology Journals
History 
  • Received April 8, 2015
  • Accepted April 9, 2015
  • Published online June 18, 2015.

Copyright & Usage 
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Editors and / or Reviewers 
  • N. A. Ledeboer, Editor

Article Versions

  • Accepted Manuscript version (April 15, 2015).
  • You are viewing the most recent version of this article.

Author Information

  1. James Heng Chiak Sima,
  2. Victoria Aniksta,
  3. Akshar Lohithb,
  4. Nader Pourmandb and
  5. Niaz Banaeia,c,d
  1. aDepartment of Pathology, Stanford University, Stanford, California, USA
  2. bDepartment of Biomolecular Engineering, University of California, Santa Cruz, California, USA
  3. cDepartment of Medicine, Stanford University, Stanford, California, USA
  4. dClinical Microbiology Laboratory, Stanford Health Care, Palo Alto, California, USA
  • Address correspondence to Niaz Banaei, nbanaei{at}stanford.edu.
  • Citation Sim JHC, Anikst V, Lohith A, Pourmand N, Banaei N. 2015. Optimized protocol for simple extraction of high-quality genomic DNA from Clostridium difficile for whole-genome sequencing. J Clin Microbiol 53:2329–2331. doi:10.1128/JCM.00956-15.

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Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing
James Heng Chiak Sim, Victoria Anikst, Akshar Lohith, Nader Pourmand, Niaz Banaei
Journal of Clinical Microbiology Jun 2015, 53 (7) 2329-2331; DOI: 10.1128/JCM.00956-15

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Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing
James Heng Chiak Sim, Victoria Anikst, Akshar Lohith, Nader Pourmand, Niaz Banaei
Journal of Clinical Microbiology Jun 2015, 53 (7) 2329-2331; DOI: 10.1128/JCM.00956-15
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