Bacteriology

Optimized Protocol for Simple Extraction of High-Quality Genomic DNA from Clostridium difficile for Whole-Genome Sequencing

James Heng Chiak Sim, Victoria Anikst, Akshar Lohith, Nader Pourmand, Niaz Banaei
N. A. Ledeboer, Editor
DOI: 10.1128/JCM.00956-15

Article Figures & Data

Figures

  • FIG 1
    FIG 1

    Gel electrophoresis results for genomic DNA (gDNA) extracted from Clostridium difficile using three different QIAamp protocols. Gram-positive protocol (A), lanes 1 to 4: gDNA extracts from isolates 38A2 (lane 1 and 2) and 69B1 (lane 3 and 4). Each lane shows an independent extraction. Bacterial suspension protocol (B), lanes 1 to 3: gDNA extracts from isolate 38A2 obtained in three independent extractions. Optimized protocol (C), lanes 1 to 6: gDNA extracts from isolates 127A4, 161A1, 208A2, 208A3, 224C1, and 224A3, respectively. M, molecular-weight marker.

Tables

  • TABLE 1

    Recovered quantity of gDNA and A260/A280 absorbance ratio for gDNA extracted using the optimized protocol

    Strain no.Quantity (μg)A260/A280 ratio
    127A418.71.81
    161A115.81.81
    208A217.21.81
    208A313.11.80
    224C115.61.81
    224A314.61.85
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