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Bacteriology

Evaluation of New bioMérieux Chromogenic CPS Media for Detection of Urinary Tract Pathogens

Josselin Rigaill, Paul O. Verhoeven, Caroline Mahinc, Mohamed Jeraiby, Florence Grattard, Nathalie Fonsale, Bruno Pozzetto, Anne Carricajo
A. B. Onderdonk, Editor
Josselin Rigaill
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, France
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Paul O. Verhoeven
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, FranceGIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), Faculty of Medicine of Saint-Etienne, University of Lyon, Saint-Etienne, France
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Caroline Mahinc
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, France
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Mohamed Jeraiby
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, France
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Florence Grattard
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, FranceGIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), Faculty of Medicine of Saint-Etienne, University of Lyon, Saint-Etienne, France
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Nathalie Fonsale
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, France
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Bruno Pozzetto
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, FranceGIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), Faculty of Medicine of Saint-Etienne, University of Lyon, Saint-Etienne, France
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Anne Carricajo
Laboratory of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, Saint-Etienne, FranceGIMAP EA 3064 (Groupe Immunité des Muqueuses et Agents Pathogènes), Faculty of Medicine of Saint-Etienne, University of Lyon, Saint-Etienne, France
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A. B. Onderdonk
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DOI: 10.1128/JCM.00941-15
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ABSTRACT

Four chromogenic media were compared for their ability to detect urinary tract pathogens in 299 urine specimens, of which 175 were found positive, allowing the growth of 279 microorganisms. After 18 to 24 h of incubation, the CPS ID4, CPSE, CPSO (bioMérieux), and UriSelect4 (Bio-Rad) media showed sensitivities of 97.1%, 99.3%, 99.6%, and 99.6%, respectively.

TEXT

It has long been recognized that the use of chromogenic agar plates improves the detection and identification of urinary tract pathogens (1–3) for the diagnosis of urinary infection. However, improvements are still needed to allow an easier differentiation of colonies in mixed culture, enhance the growth rate, and permit the detection of fastidious microorganisms.

Two chromogenic CPS media (bioMérieux) with CPSE (translucent agar) and CPSO (opaque agar) were recently commercialized, with the objectives of increasing the sensitivity of detection of microorganisms and providing results after a shortened incubation period (16 to 18 h instead of 18 to 24 h). The present study reports the performance of these two new CPS media compared with that of the CPS ID4 medium from the same company and the UriSelect4 medium from Bio-Rad.

A total of 299 urine samples were prospectively included in the study between May and July 2014. Clear urine samples were excluded. A 10-μl volume of specimen was plated onto the 4 chromogenic media. All plates were incubated at 36°C under aerobic conditions and then were visually examined after 18 to 24 h of incubation. An intermediate reading was performed at 16 to 18 h for the two new CPS media, as proposed by the manufacturer. The different types of colonies were submitted to a presumptive identification, according to their cultural characteristics (Escherichia coli, enterococci, coliform bacilli [Klebsiella, Enterobacter, Serratia, and Citrobacter], and Proteus for pink, turquoise, green, and brown colonies, respectively) and formally identified at the species level by matrix-assisted laser desorption ionization–time of flight mass spectrometry (Microflex LT; Bruker, Bremen, Germany). A urine sample was considered positive if a microorganism grew at a concentration of ≥103 CFU/ml (4) on at least one of the 4 media. Statistical analyses were performed using the MedCalc statistical software version 14.12.0 (MedCalc Software bvba, Ostend, Belgium). P values of <0.05 were considered statistically significant.

Among the 299 included samples, 88 (29.4%) tested sterile, 16 (5.4%) grew <103 CFU/ml of bacteria, and 195 (65.2%) were found positive. Among the positive samples, 131, 50, 8, and 6 yielded 1, 2, 3, and 4 microorganisms, respectively, for a total of 279 microorganisms. With a huge predominance of Enterobacteriaceae (57.7%) (of which 61.9% were E. coli), 33.3% Gram-positive bacteria, and 6.5% yeasts, the study population was representative of the distribution of urinary tract pathogens isolated in hospitals (5, 6).

After 18 to 24 h of incubation, the new CPS media and the UriSelect4 medium showed similar performance, with a sensitivity of 99.3% (277/279) for CPSE and 99.6% (278/279) for CPSO and UriSelect4, whereas the CPS ID4 medium, which exhibited a sensitivity of 97.1% (271/279), was statistically less efficient (P < 0.05). After 16 to 18 h of incubation, the two new CPS media showed a statistically significant decrease in sensitivity (94.6% [264/279] for CPSE and 95.0% [265/279] for CPSO) compared to that observed after 18 to 24 h (P < 0.05), except for Enterobacteriaceae, for which the sensitivity was 98.1% (158/161) at 16 to 18 h (Table 1). As observed in other studies (7–9), Gram-positive bacteria and yeasts exhibited delayed growth on chromogenic media (Table 1) and yielded smaller colonies than those of other species.

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TABLE 1

Microorganisms detected in urinary specimens on each of the four chromogenic media tested comparatively in this study

As recommended by the two manufacturers, pink to burgundy colonies could be identified as E. coli without needing further analysis. For this agent, a sensitivity of 99.0% was observed with the CPS ID4 medium, and a sensitivity of 98.1% was observed with the three other media; indeed, in one sample, an E. coli strain gave pale brown colonies on all four media, whereas another strain of the same species gave white colonies on all of the media except on the CPS ID4, which exhibited pink colonies. The specificity of the direct identification of E. coli was of 99.1% for the four media, since a strain of Hafnia alvei grew with pink colonies, an observation previously made with the same species (10).

Overall, the two new CPS media are efficient in detecting and identifying urinary tract pathogens. They exhibit an improvement in sensitivity compared to that of the former CPS ID4 medium. At 16 to 18 h, 95% of the urinary pathogens were recovered using the chromID CPS Elite medium, including all E. coli strains, which represents the most frequently isolated species in urinary tract infections. At 18 to 24 h, the recovery rate reaches 99.6% for chromID CPS Elite opaque and 99.3% for chromID CPS Elite translucent. However, in order to avoid missing microorganisms with slower growth, we recommend performing an additional culture of the plates at 18 to 24 h. Further studies with automated reading of cultures would permit an optimal evaluation of the sensitivity of the new media after a shorter incubation period.

ACKNOWLEDGMENTS

The media used in this study were provided by bioMérieux.

We declare no conflicts of interest related to this study.

FOOTNOTES

    • Received 7 April 2015.
    • Returned for modification 6 May 2015.
    • Accepted 14 May 2015.
    • Accepted manuscript posted online 20 May 2015.
  • Copyright © 2015, American Society for Microbiology. All Rights Reserved.

REFERENCES

  1. 1.↵
    1. Perry JD,
    2. Freydière AM
    . 2007. The application of chromogenic media in clinical microbiology. J Appl Microbiol 103:2046–2055. doi:10.1111/j.1365-2672.2007.03442.x.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Chang J-C,
    2. Chien M-L,
    3. Chen H-M,
    4. Yan J-J,
    5. Wu J-J
    . 2008. Comparison of CPS ID 3 and CHROMagar Orientation chromogenic agars with standard biplate technique for culture of clinical urine samples. J Microbiol Immunol Infect 41:422–427.
    OpenUrlPubMed
  3. 3.↵
    1. Scarparo C,
    2. Piccoli P,
    3. Ricordi P,
    4. Scagnelli M
    . 2002. Comparative evaluation of two commercial chromogenic media for detection and presumptive identification of urinary tract pathogens. Eur J Clin Microbiol Infect Dis 21:283–289. doi:10.1007/s10096-002-0718-0.
    OpenUrlCrossRefPubMedWeb of Science
  4. 4.↵
    1. Nicolle LE,
    2. Bradley S,
    3. Colgan R,
    4. Rice JC,
    5. Schaeffer A,
    6. Hooton TM
    . 2005. Infectious Diseases Society of America guidelines for the diagnosis and treatment of symptomatic bacteriuria in adults. Clin Infect Dis 40:643–654. doi:10.1086/427507.
    OpenUrlCrossRefPubMedWeb of Science
  5. 5.↵
    1. Huoi C,
    2. Vanhems P,
    3. Nicolle M-C,
    4. Michallet M,
    5. Bénet T
    . 2013. Incidence of hospital-acquired pneumonia, bacteraemia and urinary tract infections in patients with haematological malignancies, 2004–2010: a surveillance-based study. PLoS One 8:e58121. doi:10.1371/journal.pone.0058121.
    OpenUrlCrossRefPubMed
  6. 6.↵
    1. Payne M,
    2. Roscoe D
    . 2015. Evaluation of two chromogenic media for the isolation and identification of urinary tract pathogens. Eur J Clin Microbiol Infect Dis 34:303–308. doi:10.1007/s10096-014-2235-3.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Carricajo A,
    2. Boiste S,
    3. Thore J,
    4. Aubert G,
    5. Gille Y,
    6. Freydière AM
    . 1999. Comparative evaluation of five chromogenic media for detection, enumeration and identification of urinary tract pathogens. Eur J Clin Microbiol Infect Dis 18:796–803. doi:10.1007/s100960050403.
    OpenUrlCrossRefPubMedWeb of Science
  8. 8.↵
    1. Chaux C,
    2. Crepy M,
    3. Xueref S,
    4. Roure C,
    5. Gille Y,
    6. Freydiere AM
    . 2002. Comparison of three chromogenic agar plates for isolation and identification of urinary tract pathogens. Clin Microbiol Infect 8:641–645. doi:10.1046/j.1469-0691.2002.00433.x.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Perry JD,
    2. Butterworth LA,
    3. Nicholson A,
    4. Appleby MR,
    5. Orr KE
    . 2003. Evaluation of a new chromogenic medium, UriSelect 4, for the isolation and identification of urinary tract pathogens. J Clin Pathol 56:528–531. doi:10.1136/jcp.56.7.528.
    OpenUrlAbstract/FREE Full Text
  10. 10.↵
    1. Meddeb M,
    2. Maurer M,
    3. Grillon A,
    4. Scheftel J-M,
    5. Jaulhac B
    . 2014. Comparaison de l'utilisation en routine de deux milieux chromogènes ChromID CPS (bioMerieux) et UriSelect4 (Bio-Rad) pour la détection d'Escherichia coli et des principaux uropathogènes dans les urines. Ann Biol Clin (Paris) 72:224–230. doi:10.1684/abc.2014.0943.
    OpenUrlCrossRefPubMed
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Evaluation of New bioMérieux Chromogenic CPS Media for Detection of Urinary Tract Pathogens
Josselin Rigaill, Paul O. Verhoeven, Caroline Mahinc, Mohamed Jeraiby, Florence Grattard, Nathalie Fonsale, Bruno Pozzetto, Anne Carricajo
Journal of Clinical Microbiology Jul 2015, 53 (8) 2701-2702; DOI: 10.1128/JCM.00941-15

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Evaluation of New bioMérieux Chromogenic CPS Media for Detection of Urinary Tract Pathogens
Josselin Rigaill, Paul O. Verhoeven, Caroline Mahinc, Mohamed Jeraiby, Florence Grattard, Nathalie Fonsale, Bruno Pozzetto, Anne Carricajo
Journal of Clinical Microbiology Jul 2015, 53 (8) 2701-2702; DOI: 10.1128/JCM.00941-15
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