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Bacteriology

Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial

Hossein Salimnia, Marilynn R. Fairfax, Paul R. Lephart, Paul Schreckenberger, Sharon M. DesJarlais, J. Kristie Johnson, Gwen Robinson, Karen C. Carroll, Amy Greer, Margie Morgan, Raymond Chan, Michael Loeffelholz, Frances Valencia-Shelton, Stephen Jenkins, Audrey N. Schuetz, Judy A. Daly, Trenda Barney, Andrew Hemmert, Kristen J. Kanack
S. S. Richter, Editor
Hossein Salimnia
Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan, USADMC University Laboratories, Detroit, Michigan, USA
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Marilynn R. Fairfax
Department of Pathology, Wayne State University School of Medicine, Detroit, Michigan, USADMC University Laboratories, Detroit, Michigan, USA
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Paul R. Lephart
DMC University Laboratories, Detroit, Michigan, USA
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Paul Schreckenberger
Loyola University Medical Center, Chicago, Illinois, USA
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Sharon M. DesJarlais
Loyola University Medical Center, Chicago, Illinois, USA
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J. Kristie Johnson
Department of Pathology, University of Maryland, Baltimore, Maryland, USA
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Gwen Robinson
Department of Pathology, University of Maryland, Baltimore, Maryland, USA
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Karen C. Carroll
Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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Amy Greer
Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
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Margie Morgan
Cedars-Sinai Medical Center, Los Angeles, California, USA
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Raymond Chan
Cedars-Sinai Medical Center, Los Angeles, California, USA
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Michael Loeffelholz
Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA
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Frances Valencia-Shelton
Department of Pathology, University of Texas Medical Branch, Galveston, Texas, USA
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Stephen Jenkins
New York-Presbyterian Hospital, Weill-Cornell Medical Center, New York, New York, USA
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Audrey N. Schuetz
New York-Presbyterian Hospital, Weill-Cornell Medical Center, New York, New York, USA
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Judy A. Daly
Department of Pathology, University of Utah, and Primary Children's Medical Center, Salt Lake City, Utah, USA
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Trenda Barney
Department of Pathology, University of Utah, and Primary Children's Medical Center, Salt Lake City, Utah, USA
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Andrew Hemmert
BioFire Diagnostics LLC, Salt Lake City, Utah, USA
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Kristen J. Kanack
BioFire Diagnostics LLC, Salt Lake City, Utah, USA
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S. S. Richter
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DOI: 10.1128/JCM.01679-15
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ABSTRACT

Sepsis is a major cause of morbidity, mortality, and increased medical expense. Rapid diagnosis improves outcomes and reduces costs. The FilmArray blood culture identification panel (BioFire Diagnostics LLC, Salt Lake City, UT), a highly multiplexed PCR assay, can identify 24 etiologic agents of sepsis (8 Gram-positive, 11 Gram-negative, and 5 yeast species) and three antimicrobial resistance genes (mecA, vanA/B, and bla KPC) from positive blood culture bottles. It provides results in about 1 h with 2 min for assay setup. We present the results of an eight-center trial comparing the sensitivity and specificity of the panel with those of the laboratories' standard phenotypic identification techniques, as well as with molecular methods used to distinguish Acinetobacter baumannii from other members of the A. calcoaceticus-A. baumannii complex and to detect antimicrobial resistance genes. Testing included 2,207 positive aerobic blood culture samples, 1,568 clinical and 639 seeded. Samples were tested fresh or were frozen for later testing within 8 h after the bottles were flagged as positive by an automated blood culture system. At least one organism was detected by the panel in 1,382 (88.1%) of the positive clinical specimens. The others contained primarily off-panel organisms. The panel reported multiple organisms in 81 (5.86%) positive clinical specimens. The unresolved blood culture identification sensitivity for all target detections exceeded 96%, except for Klebsiella oxytoca (92.2%), which achieved 98.3% sensitivity after resolution of an unavoidable phenotypic error. The sensitivity and specificity for vanA/B and bla KPC were 100%; those for mecA were 98.4 and 98.3%, respectively.

  • Copyright © 2016 Salimnia et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial
Hossein Salimnia, Marilynn R. Fairfax, Paul R. Lephart, Paul Schreckenberger, Sharon M. DesJarlais, J. Kristie Johnson, Gwen Robinson, Karen C. Carroll, Amy Greer, Margie Morgan, Raymond Chan, Michael Loeffelholz, Frances Valencia-Shelton, Stephen Jenkins, Audrey N. Schuetz, Judy A. Daly, Trenda Barney, Andrew Hemmert, Kristen J. Kanack
Journal of Clinical Microbiology Feb 2016, 54 (3) 687-698; DOI: 10.1128/JCM.01679-15

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Evaluation of the FilmArray Blood Culture Identification Panel: Results of a Multicenter Controlled Trial
Hossein Salimnia, Marilynn R. Fairfax, Paul R. Lephart, Paul Schreckenberger, Sharon M. DesJarlais, J. Kristie Johnson, Gwen Robinson, Karen C. Carroll, Amy Greer, Margie Morgan, Raymond Chan, Michael Loeffelholz, Frances Valencia-Shelton, Stephen Jenkins, Audrey N. Schuetz, Judy A. Daly, Trenda Barney, Andrew Hemmert, Kristen J. Kanack
Journal of Clinical Microbiology Feb 2016, 54 (3) 687-698; DOI: 10.1128/JCM.01679-15
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