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Letter to the Editor

Genotyping of Aspergillus fumigatus Reveals Compartmentalization of Genotypes in Disseminated Disease after Invasive Pulmonary Aspergillosis

Pilar Escribano, Patricia Muñoz, Pedro Montilla, Belén Padilla, Emilio Bouza, Jesús Guinea
Daniel J. Diekema, Editor
Pilar Escribano
aClinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain
bInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Madrid, Spain
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Patricia Muñoz
aClinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain
bInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Madrid, Spain
cCIBER de Enfermedades Respiratorias (CIBERES CB06/06/0058), Madrid, Spain
dMedicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain
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Pedro Montilla
aClinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain
bInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Madrid, Spain
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Belén Padilla
aClinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain
bInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Madrid, Spain
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Emilio Bouza
aClinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain
bInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Madrid, Spain
cCIBER de Enfermedades Respiratorias (CIBERES CB06/06/0058), Madrid, Spain
dMedicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain
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Jesús Guinea
aClinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Marañón, Universidad Complutense de Madrid, Madrid, Spain
bInstituto de Investigación Sanitaria del Hospital Gregorio Marañón, Madrid, Spain
cCIBER de Enfermedades Respiratorias (CIBERES CB06/06/0058), Madrid, Spain
dMedicine Department, School of Medicine, Universidad Complutense de Madrid, Madrid, Spain
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Daniel J. Diekema
University of Iowa College of Medicine
Roles: Editor
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DOI: 10.1128/JCM.02037-16
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LETTER

Invasive pulmonary aspergillosis (IPA) disseminated to distant organs (IPAd) is uncommon (1, 2) and has traditionally been considered a monomicrobial infection caused by a single Aspergillus fumigatus clone present both in the lower respiratory tract and at distant sites (3, 4). In order to explore the potential compartmentalization of genotypes at different anatomical sites, we studied available isolates collected from the lung and distant sites in 3 patients with proven IPAd (5, 6) (Table 1). A. fumigatus colonies (n = 38) from 10 samples were subcultured for molecular identification (7), genotyping using short tandem repeats of A. fumigatus (STRAf genotyping) (8), and antifungal susceptibility testing (9).

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TABLE 1

Underlying conditions of and diagnostic criteria of aspergillosis for the 3 patients and genotypes found in samples from the lower respiratory tract and distant organsa

(The findings of this study were partially presented at the 23rd European Congress of Clinical Microbiology and Infectious Diseases [ECCMID], Berlin, Germany, 27 to 30 April 2013 [abstr O-467] [10]).

Microsatellite typing of the isolates resulted in the detection of 10 genotypes. Lower respiratory tract samples tended to yield several genotypes, whereas nonrespiratory samples yielded a single genotype. Patient 1 was a 26-year-old woman who had received an allogeneic hematopoietic stem cell transplant for acute lymphocytic leukemia in 2004. Her disease relapsed in 2006, and she received chemotherapy and corticosteroids to control graft-versus-host disease. IPAd was proven in the autopsy, and a single genotype was found in the 2 samples. Data for patient 2 have been reported elsewhere (11). IPAd was diagnosed based on the isolation of A. fumigatus in lower respiratory tract samples and blood cultures. A total of 3 genotypes were found in the lung, but only 1 of them was present in the blood cultures; this genotype was found in several lower respiratory tract samples and was clonally related to another genotype found in 2 additional respiratory samples. Patient 3 was a homeless person who had received corticosteroids. He was diagnosed first with IPA (probable) and then with invasive aspergillosis of the brain 16 months later (proven). Of the 6 genotypes found in the lung during the first episode, 1 was detected in the central nervous system sample taken during the second episode.

All isolates were susceptible to itraconazole, voriconazole, and posaconazole. We did not find differences in antifungal susceptibility between different colonies from the same patient or between the genotypes. Genotyping enabled us to explore 3 issues with clinical impact. First, our data support the hypothesis of dissemination of disease from the lung. Second, the presence of identical genotypes in the blood and respiratory tract in patient 2 sustains the idea of the potential clinical significance of isolating A. fumigatus from blood cultures in a patient with risk factors and symptoms compatible with invasive aspergillosis (5, 11). Finally, patient 3 had 2 episodes of IPA in 16 months; since the second episode was caused by 1 of the genotypes found in the lung during the first episode, the second episode was a relapse rather than a reinfection. We observed compartmentalization of A. fumigatus in patients with IPAd; the genotype found at distant sites was always present in the respiratory tract, and some genotypes were found exclusively in the lung. A refined and in-depth analysis of all visible A. fumigatus isolates grown on the plates was required to support these observations.

ACKNOWLEDGMENTS

This study was supported by grant numbers PI14/00740 and MSI15/00115 from the Fondo de Investigación Sanitaria (FIS, Instituto de Salud Carlos III; Plan Nacional de I+D+I 2013-2016) and cofunded by the European Regional Development Fund (FEDER) “A way of making Europe.” The funding bodies had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. P.E. (CPI15/00115) and J.G. (CPII15/00006) hold a Miguel Servet contract.

  • Copyright © 2016 American Society for Microbiology.

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Genotyping of Aspergillus fumigatus Reveals Compartmentalization of Genotypes in Disseminated Disease after Invasive Pulmonary Aspergillosis
Pilar Escribano, Patricia Muñoz, Pedro Montilla, Belén Padilla, Emilio Bouza, Jesús Guinea
Journal of Clinical Microbiology Dec 2016, 55 (1) 331-333; DOI: 10.1128/JCM.02037-16

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Genotyping of Aspergillus fumigatus Reveals Compartmentalization of Genotypes in Disseminated Disease after Invasive Pulmonary Aspergillosis
Pilar Escribano, Patricia Muñoz, Pedro Montilla, Belén Padilla, Emilio Bouza, Jesús Guinea
Journal of Clinical Microbiology Dec 2016, 55 (1) 331-333; DOI: 10.1128/JCM.02037-16
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KEYWORDS

Aspergillus fumigatus
genotyping
disseminated lung infection
Aspergillus fumigatus
invasive pulmonary aspergillosis
Mycological Typing Techniques

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