Genotyping of Aspergillus fumigatus Reveals Compartmentalization of Genotypes in Disseminated Disease after Invasive Pulmonary Aspergillosis

Pilar Escribano; Patricia Muñoz; Pedro Montilla; Belén Padilla; Emilio Bouza; Jesús Guinea

doi:10.1128/JCM.02037-16

DOI: 10.1128/JCM.02037-16

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Tables

TABLE 1

Underlying conditions of and diagnostic criteria of aspergillosis for the 3 patients and genotypes found in samples from the lower respiratory tract and distant organs^a

Patient	Underlying condition(s)	Radiological finding(s)	Mycological finding(s)	Sample (date of isolation)	Genotype(s) (no. of colonies)
1	Lymphocytic acute leukemia; allogeneic stem cell transplantation	Nodules in the lungs on CT scan; no lesions in the brain on CT scan	Positive serum galactomannan result; isolation of A. fumigatus from the brain sample and respiratory sample	Lung biopsy (30 September 2006)	AF-1 (10)
1				Brain biopsy (30 September 2006)	AF-1 (2)
2	COPD	Opacity in the upper right lobe, with nodular lesions on the chest X-ray	Positive serum galactomannan result; isolation of A. fumigatus from the respiratory sample and blood culture	Sputum (15 January 2009)	AF-2 (2), AF-3 (2)
				Blood culture (18 January 2009)	AF-2 (3)
				BAS (19 January 2009)	AF-2 (1), AF-3 (1), AF-4 (1)
				BAS (19 January 2009)	AF-4 (2)
3	HIV (11 CD4 cells/mm³) H1N1 infection	Bilateral cavitated infiltrates on CT scan; abscesses and ventriculitis on central nervous system CT scan	Positive BAL fluid galactomannan result; isolation of A. fumigatus from the respiratory sample and blood culture and CSF samples	Sputum (26 January 2011)	AF-5 (2), AF-6 (1)
				Sputum (28 January 2011)	AF-7 (2), AF-8 (1), AF-9 (1)
				Sputum (31 January 2011)	AF-5 (1), AF-8 (2), AF-10 (2)
				CSF (28 May 2012)	AF-6 (2)

↵a Genotypes in bold indicate matches between strains from the lower respiratory tract and distant organs. All 3 patients were receiving corticosteroids and had proven aspergillosis according to European Organization for Research and Treatment of Cancer (EORTC) criteria (patients 1 and 3) (5) or the criteria of Blot et al. (patient 2) (6). BAS, basophil; COPD, chronic obstructive pulmonary disease; CSF, cerebrospinal fluid; CT, computed tomography.