Article Figures & Data
Figures
- FIG 1
Distribution of serum specimens according to ZIKV reference PCR, Altona PCR, and NS5 gene PCR results. ZIKV, Zika virus; reference PCR, 2-step dual-target real-time reverse transcription-PCR, designed by the U.S. Centers for Disease Control and Prevention, targeting the Zika virus envelope (E) and premembrane (prM) genes; NS5, nonstructural protein 5; NSQ, insufficient quantity for testing.
- FIG 2
(A) Serum ZIKV reference PCR positivity by day following symptom onset. (B) CT values of E and prM gene targets by day following symptom onset. ZIKV, Zika virus; reference PCR, 2-step dual-target real-time reverse transcription-PCR, designed by the U.S. Center for Disease Control and Prevention, targeting the Zika virus envelope (E) and premembrane (prM) genes; CT, cycle threshold. The horizontal bars represent median CT values.
- FIG 3
Distribution of urine specimens according to ZIKV reference PCR, Altona PCR, and NS5 gene PCR results. ZIKV, Zika virus; reference PCR, 2-step dual-target real-time reverse transcription-PCR, designed by the U.S. Centers for Disease Control and Prevention, targeting the Zika virus envelope (E) and premembrane (prM) genes; NS5, nonstructural protein 5; NT, not tested.
- FIG 4
ZIKV genome and locations targeted by the ZIKV reference PCR, Altona PCR, and NS5 gene PCR. ZIKV, Zika virus; reference PCR, 2-step dual-target real-time reverse transcription-PCR, designed by the U.S. Centers for Disease Control and Prevention, targeting the Zika virus envelope (E) and premembrane (prM) genes; NS, nonstructural protein gene; C, capsid protein gene. The schematic representation depicts the coding and untranslated regions (UTRs) of the ZIKV genome. The three structural proteins C, prM, and E and the nonstructural (NS) proteins NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5 are specified. The assay targets are indicated above. Briefly, the reference PCR dual-target assay amplifies a 75-bp region spanning the prM-E boundary (Zika1) and a 76-bp portion of the E gene (Zika2), the NS5 PCR targets a 191-bp internal region of the NS5 gene, and the Altona PCR targets the NS1 gene (personal communication with the manufacturer). The viral schematic and genomic locations are based on Zika virus reference strain MR-766 (GenBank accession no. NC_012532.1 ).
