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Bacteriology

Identification of Virulent Capnocytophaga canimorsus Isolates by Capsular Typing

Estelle Hess, Francesco Renzi, Dunia Koudad, Mélanie Dol, Guy R. Cornelis
Robin Patel, Editor
Estelle Hess
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Francesco Renzi
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Dunia Koudad
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Mélanie Dol
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Guy R. Cornelis
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Robin Patel
Mayo Clinic
Roles: Editor
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DOI: 10.1128/JCM.00249-17
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ABSTRACT

Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We describe nine different serovars (A to I), and this serotyping scheme allowed typing of 25/25 isolates from human infections but only 18/52 isolates from dog mouths, indicating that the repertoire of capsules in the species is vast. However, while only three serovars (A, B, and C) covered 88% of the human isolates tested (22/25), they covered only 7.7% of the dog isolates (4/52). Serovars A, B, and C were found 22.9-, 14.6-, and 4.2-fold more often, respectively, among human isolates than among dog isolates, with no geographical bias, implying that isolates endowed with these three capsular types are more virulent for humans than other isolates. Capsular serotyping would thus allow identification of virulent isolates in dogs, which could contribute to the prevention of these infections. To this end, we developed a PCR typing method based on the amplification of specific capsular genes.

FOOTNOTES

    • Received 18 February 2017.
    • Returned for modification 17 March 2017.
    • Accepted 2 April 2017.
    • Accepted manuscript posted online 5 April 2017.
  • Supplemental material for this article may be found at https://doi.org/10.1128/JCM.00249-17 .

  • Copyright © 2017 Hess et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license .

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Identification of Virulent Capnocytophaga canimorsus Isolates by Capsular Typing
Estelle Hess, Francesco Renzi, Dunia Koudad, Mélanie Dol, Guy R. Cornelis
Journal of Clinical Microbiology May 2017, 55 (6) 1902-1914; DOI: 10.1128/JCM.00249-17

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Identification of Virulent Capnocytophaga canimorsus Isolates by Capsular Typing
Estelle Hess, Francesco Renzi, Dunia Koudad, Mélanie Dol, Guy R. Cornelis
Journal of Clinical Microbiology May 2017, 55 (6) 1902-1914; DOI: 10.1128/JCM.00249-17
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KEYWORDS

Bacterial Typing Techniques
Capnocytophaga
Gram-Negative Bacterial Infections
Polysaccharides, Bacterial
serotyping
virulence factors
capsular polysaccharide
ELISA
PCR
septicemia
serotyping scheme
Western blot

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