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Virology

Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens

R. H. T. Nijhuis, D. Guerendiain, E. C. J. Claas, K. E. Templeton
Alexander J. McAdam, Editor
R. H. T. Nijhuis
aDepartment of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
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D. Guerendiain
bSpecialist Virology Center, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom
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E. C. J. Claas
aDepartment of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
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K. E. Templeton
bSpecialist Virology Center, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom
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Alexander J. McAdam
Boston Children's Hospital
Roles: Editor
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DOI: 10.1128/JCM.00221-17
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ABSTRACT

Infections of the respiratory tract can be caused by a diversity of pathogens, both viral and bacterial. Rapid microbiological diagnosis ensures appropriate antimicrobial therapy as well as effective implementation of isolation precautions. The ePlex respiratory pathogen panel (RP panel) is a novel molecular biology-based assay, developed by GenMark Diagnostics, Inc. (Carlsbad, CA), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). The objective of this study was to compare the performance of the RP panel with those of laboratory-developed real-time PCR assays, using a variety of previously collected clinical respiratory specimens. A total of 343 clinical specimens as well as 29 external quality assessment (EQA) specimens and 2 different Middle East respiratory syndrome coronavirus isolates have been assessed in this study. The RP panel showed an agreement of 97.4% with the real-time PCR assay regarding 464 pathogens found in the clinical specimens. All pathogens present in clinical samples and EQA samples with a threshold cycle (CT) value of <30 were detected correctly using the RP panel. The RP panel detected 17 additional pathogens, 7 of which could be confirmed by discrepant testing. In conclusion, this study shows excellent performance of the RP panel in comparison to real-time PCR assays for the detection of respiratory pathogens. The ePlex system provided a large amount of useful diagnostic data within a short time frame, with minimal hands-on time, and can therefore potentially be used for rapid diagnostic sample-to-answer testing, in either a laboratory or a decentralized setting.

  • Copyright © 2017 Nijhuis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license .

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Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens
R. H. T. Nijhuis, D. Guerendiain, E. C. J. Claas, K. E. Templeton
Journal of Clinical Microbiology May 2017, 55 (6) 1938-1945; DOI: 10.1128/JCM.00221-17

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Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens
R. H. T. Nijhuis, D. Guerendiain, E. C. J. Claas, K. E. Templeton
Journal of Clinical Microbiology May 2017, 55 (6) 1938-1945; DOI: 10.1128/JCM.00221-17
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KEYWORDS

bacterial infections
Molecular Diagnostic Techniques
real-time polymerase chain reaction
respiratory tract infections
Virus Diseases
influenza
point-of-care testing
rapid diagnostics
respiratory pathogens
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