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Virology

Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens

R. H. T. Nijhuis, D. Guerendiain, E. C. J. Claas, K. E. Templeton
Alexander J. McAdam, Editor
R. H. T. Nijhuis
aDepartment of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
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D. Guerendiain
bSpecialist Virology Center, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom
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E. C. J. Claas
aDepartment of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
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K. E. Templeton
bSpecialist Virology Center, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom
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Alexander J. McAdam
Boston Children's Hospital
Roles: Editor
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DOI: 10.1128/JCM.00221-17
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  • FIG 1
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    FIG 1

    ePlex system (A) and the corresponding cartridge of the ePlex respiratory pathogen panel (B).

  • FIG 2
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    FIG 2

    Principle of the eSensor detection technology. Amplified sequences of the targeted pathogens are detected electrochemically using a complementary pathogen-specific signal probe tagged with ferrocene, a reducing agent. The hybridized molecule is then exposed to another sequence-specific probe that is bound to a solid phase, which is a gold electrode (A). Upon binding of the two molecules, the ferrocene comes in close proximity to the gold electrode, where an electron transfer that can be measured using GenMark's eSensor technology on the ePlex system can occur (B).

  • FIG 3
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    FIG 3

    Pathogen concordance by CT value.

Tables

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  • TABLE 1

    Pathogens included in this study

    RP panel targetNo. of results
    Found in clinical specimensPCR+/RP−PCR−/RP+
    Viral
        Adenovirus39 4
        Coronavirus
            229E711
            HKU1121
            NL637 1
            OC43911
            MERS coronavirus
        Human bocavirus2731
        Human metapneumovirus283
        Human rhinovirus/enterovirusa134b2c6a
        Influenza virus
            Influenza A1d
                H15
                2009 H1N118 1e
                H317
            Influenza B20
        Parainfluenza virus
            Type 111
            Type 2121
            Type 315
            Type 42 2
        Respiratory syncytial virus13d
            Type A43
            Type B24
    Bacterial
        Bordetella pertussis6
        Chlamydophila pneumoniae0
        Legionella pneumophila6
        Mycoplasma pneumoniae8
    • ↵a No differentiation possible between rhinovirus and enterovirus.

    • ↵b One hundred seven rhinoviruses and 27 enteroviruses found by routine testing.

    • ↵c Both rhinovirus.

    • ↵d No further subtyping performed.

    • ↵e In the laboratory-developed test detected as influenza A virus.

  • TABLE 2

    Non-NPSa specimens included in this study

    Specimen typeNo. includedPathogen (n)
    Sputum21Human rhinovirus (10)
    Legionella pneumophila (6)
    Human metapneumovirus (3)
    Respiratory syncytial virus (1)
    Influenza A virus H1N1 (1)
    Influenza A virus H3 (1)
    Mycoplasma pneumoniae (1)
    Bronchoalveolar lavage fluids16Human rhinovirus (12)
    Human metapneumovirus (3)
    Enterovirus (2)
    Human bocavirus (2)
    Respiratory syncytial virus (1)
    Mycoplasma pneumoniae (1)
    Adenovirus (1)
    Throat swab10Human rhinovirus (7)
    Respiratory syncytial virus (4)
    Enterovirus (1)
    Nasopharyngeal aspirate10Respiratory syncytial virus (7)
    Human rhinovirus (3)
    Enterovirus (1)
    Adenovirus (1)
    • ↵a NPS, nasopharyngeal swab.

  • TABLE 3

    EQA samples included in the study

    EQA sampleContentaRP panel
    RESPII16-01Rhinovirus type 72 d1Rhinovirus/enterovirus
    RESPII16-02Rhinovirus type 72 d2Rhinovirus/enterovirus
    RESPII16-03NegativeNegative
    RESPII16-04Adenovirus type 1Adenovirus
    RESPII16-05Coronavirus OC43Coronavirus OC43
    RESPII16-06 b Human metapneumovirus and coronavirus NL63 Human metapneumovirus
    RESPII16-07Rhinovirus type 8Rhinovirus/enterovirus
    RESPII16-08Parainfluenza virus type 3 and rhinovirus type 72Parainfluenza virus type 3 and rhinovirus/enterovirus
    RESPII16-09Human metapneumovirusHuman metapneumovirus
    RESPII16-10Coronavirus NL63 d1Coronavirus NL63
    RESPII16-11 Coronavirus NL63 d2 Negative
    RESPII16-12Parainfluenza virus type 1Parainfluenza virus type 1
    Qnostics RSP-S01Influenza A virusInfluenza A virus
    Qnostics RSP-S02Influenza B virusInfluenza B virus
    Qnostics RSP-S03Respiratory syncytial virus type ARespiratory syncytial virus type A
    Qnostics RSP-S04Respiratory syncytial virus type BRespiratory syncytial virus type B
    Qnostics RSP-S05 Adenovirus type 1 Negative
    Qnostics RSP-S06Coronavirus NL63Coronavirus NL63
    Qnostics RSP-S07Coronavirus OC43Coronavirus OC43
    Qnostics RSP-S08Parainfluenza virus type 1Parainfluenza virus type 1
    Qnostics RSP-S09Parainfluenza virus type 3 and rhinovirusParainfluenza virus type 3 and rhinovirus/enterovirus
    Qnostics RSP-S10Human metapneumovirusHuman metapneumovirus
    Qnostics RSP-S11RhinovirusRhinovirus/enterovirus
    Qnostics RSP-S12 Bordetella pertussis Bordetella pertussis
    Qnostics RSP-S13 Chlamydophila pneumoniae Negative
    Qnostics RSP-S14 Mycoplasma pneumoniae Mycoplasma pneumoniae
    Qnostics RSP-S15 Legionella pneumophila Legionella pneumophila
    Qnostics RSP-S16 Legionella pneumophila Legionella pneumophila
    Qnostics RSP-S17NegativeNegative
    • ↵a d1 and d2, different dilutions of the same virus, no information on the concentration provided.

    • ↵b Data in bold indicate samples that incorrectly detected the (full) content of the EQA sample.

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Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens
R. H. T. Nijhuis, D. Guerendiain, E. C. J. Claas, K. E. Templeton
Journal of Clinical Microbiology May 2017, 55 (6) 1938-1945; DOI: 10.1128/JCM.00221-17

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Comparison of ePlex Respiratory Pathogen Panel with Laboratory-Developed Real-Time PCR Assays for Detection of Respiratory Pathogens
R. H. T. Nijhuis, D. Guerendiain, E. C. J. Claas, K. E. Templeton
Journal of Clinical Microbiology May 2017, 55 (6) 1938-1945; DOI: 10.1128/JCM.00221-17
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KEYWORDS

bacterial infections
Molecular Diagnostic Techniques
real-time polymerase chain reaction
respiratory tract infections
Virus Diseases
influenza
point-of-care testing
rapid diagnostics
respiratory pathogens
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