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The Brief Case

The Brief Case: Staphylococcus intermedius Group—Look What the Dog Dragged In

William Lainhart, Melanie L. Yarbrough, Carey-Ann D. Burnham
Alexander J. McAdam, Editor
William Lainhart
aDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA
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Melanie L. Yarbrough
aDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA
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Carey-Ann D. Burnham
aDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri, USA
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Alexander J. McAdam
Boston Children's Hospital
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DOI: 10.1128/JCM.00839-17
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    FIG 1

    Colony morphologies of Staphylococcus intermedius group members and Staphylococcus aureus at 24 h of growth. (A) Colony morphology of S. aureus on a sheep blood agar plate. (B) Beta-hemolysis caused by S. aureus. The photo was taken using backlighting to highlight the hemolysis pattern. (C) Colony morphology of S. intermedius group on a sheep blood agar plate. (D) Double zone of beta-hemolysis caused by an S. intermedius group member. The photo was taken using backlighting to highlight the hemolysis pattern. (E) Growth of an S. intermedius group member on a Spectra MRSA chromogenic agar plate (Remel Microbiology Products, Lenexa, KS). (F) Growth of an S. intermedius group member on mannitol salt agar (Remel Microbiology Products).

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    FIG 2

    Biochemical characteristics of Staphylococcus intermedius group members compared to those of Staphylococcus aureus, and susceptibility testing for S. intermedius group members. (A) Latex agglutination test (coagulase) result for S. aureus; (B) latex agglutination test (coagulase) result for S. intermedius group members; (C) PYR testing results for S. aureus (left, negative) and S. intermedius group members (right, positive); (D) discrepant cefoxitin (FOX 30; 26-mm diameter) and oxacillin (OX 1; 15-mm diameter) results for an S. intermedius group isolate.

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  • TABLE 1

    Biochemical characteristics used to differentiate Staphylococcus aureus from animal-adapted, coagulase-positive Staphylococcus speciesa

    Staphylococcus speciesPresence of indicated characteristic
    Clumping factorFree coagulasePYRUreaseHeat-stable nucleased-Mannitol fermentationPolymyxin B resistance
    S. aureus++−V+++
    S. intermediusV++++V−
    S. pseudintermedius−+++++V
    S. delphini−+ND+−+−
    S. schleiferi subsp. schleiferi+−+−+−−
    S. schleiferi subsp. coagulans−+V++V−
    • ↵a Data are adapted from references 4 and 11. +, 90% or more strains were positive; V, results were varied; −, 90% or more strains were negative; ND, not determined.

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The Brief Case: Staphylococcus intermedius Group—Look What the Dog Dragged In
William Lainhart, Melanie L. Yarbrough, Carey-Ann D. Burnham
Journal of Clinical Microbiology Jan 2018, 56 (2) e00839-17; DOI: 10.1128/JCM.00839-17

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The Brief Case: Staphylococcus intermedius Group—Look What the Dog Dragged In
William Lainhart, Melanie L. Yarbrough, Carey-Ann D. Burnham
Journal of Clinical Microbiology Jan 2018, 56 (2) e00839-17; DOI: 10.1128/JCM.00839-17
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KEYWORDS

methicillin resistance
Staphylococcus intermedius group
Staphylococcus

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