The Utility of Multiplex Molecular Tests for Enteric Pathogens: a Micro-Comic Strip

Alexander J. McAdam

doi:10.1128/JCM.01916-17

DOI: 10.1128/JCM.01916-17

The views expressed in this Editorial do not necessarily reflect the views of the journal or of ASM.

EDITORIAL

There are several FDA-approved multiplex molecular tests available for detection of enteric pathogens in stool (1 –8). These tests allow for rapid detection of a variety of enteric pathogens; however, it can be challenging for laboratory directors to decide what pathogens to report and whether to continue to perform other tests for pathogens included in these tests (9, 10). How might laboratory directors approach implementation of these tests? Read the comic strip to find out.

REFERENCES

1.↵
1. Buchan BW,
2. Olson WJ,
3. Pezewski M,
4. Marcon MJ,
5. Novicki T,
6. Uphoff TS,
7. Chandramohan L,
8. Revell P,
9. Ledeboer NA
. 2013. Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga toxin-producing Escherichia coli isolates in stool specimens. J Clin Microbiol 51:4001–4007. doi:10.1128/JCM.02056-13.
OpenUrl Abstract/FREE Full Text
2.↵
1. Buss SN,
2. Leber A,
3. Chapin K,
4. Fey PD,
5. Bankowski MJ,
6. Jones MK,
7. Rogatcheva M,
8. Kanack KJ,
9. Bourzac KM
. 2015. Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. J Clin Microbiol 53:915–925. doi:10.1128/JCM.02674-14.
OpenUrl Abstract/FREE Full Text
3.↵
1. DeBurger B,
2. Hanna S,
3. Powell EA,
4. Ventrola C,
5. Mortensen JE
. 2017. Utilizing BD MAX enteric bacterial panel to detect stool pathogens from rectal swabs. BMC Clin Pathol 17:7. doi:10.1186/s12907-017-0043-2.
OpenUrl CrossRef
4.↵
1. Harrington SM,
2. Buchan BW
. 2015. Multicenter evaluation of the BD Max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes. J Clin Microbiol 53:1639–1647. doi:10.1128/JCM.03480-14.
OpenUrl Abstract/FREE Full Text
5.↵
1. Huang RS,
2. Johnson CL,
3. Pritchard L,
4. Hepler R,
5. Ton TT,
6. Dunn JJ
. 2016. Performance of the Verigene® enteric pathogens test, Biofire FilmArray gastrointestinal panel and Luminex xTAG® gastrointestinal pathogen panel for detection of common enteric pathogens. Diagn Microbiol Infect Dis 86:336–339. doi:10.1016/j.diagmicrobio.2016.09.013.
OpenUrl CrossRef
6.↵
1. Knabl L,
2. Grutsch I,
3. Orth-Holler D
. 2016. Comparison of the BD MAX® Enteric Bacterial Panel assay with conventional diagnostic procedures in diarrheal stool samples. Eur J Clin Microbiol Infect Dis 35:131–136. doi:10.1007/s10096-015-2517-4.
OpenUrl CrossRef PubMed
7.↵
1. Madison-Antenucci S,
2. Relich RF,
3. Doyle L,
4. Espina N,
5. Fuller D,
6. Karchmer T,
7. Lainesse A,
8. Mortensen JE,
9. Pancholi P,
10. Veros W,
11. Harrington SM
. 2016. Multicenter evaluation of BD Max enteric parasite real-time PCR assay for detection of Giardia duodenalis, Cryptosporidium hominis, Cryptosporidium parvum, and Entamoeba histolytica. J Clin Microbiol 54:2681–2688. doi:10.1128/JCM.00765-16.
OpenUrl Abstract/FREE Full Text
8.↵
1. Molling P,
2. Nilsson P,
3. Ennefors T,
4. Ogren J,
5. Floren K,
6. Thulin Hedberg S,
7. Sundqvist M
. 2016. Evaluation of the BD Max enteric parasite panel for clinical diagnostics. J Clin Microbiol 54:443–444. doi:10.1128/JCM.02100-15.
OpenUrl Abstract/FREE Full Text
9.↵
1. Binnicker MJ
. 2015. Multiplex molecular panels for diagnosis of gastrointestinal infection: performance, result interpretation, and cost-effectiveness. J Clin Microbiol 53:3723–3728. doi:10.1128/JCM.02103-15.
OpenUrl Abstract/FREE Full Text
10.↵
1. Hanson KE,
2. Couturier MR
. 2016. Multiplexed molecular diagnostics for respiratory, gastrointestinal, and central nervous system infections. Clin Infect Dis 63:1361–1367. doi:10.1093/cid/ciw494.
OpenUrl CrossRef PubMed

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KEYWORDS

enteric pathogens

molecular methods

Cited By...

[1] 1.↵
Buchan BW,
Olson WJ,
Pezewski M,
Marcon MJ,
Novicki T,
Uphoff TS,
Chandramohan L,
Revell P,
Ledeboer NA
. 2013. Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga toxin-producing Escherichia coli isolates in stool specimens. J Clin Microbiol 51:4001–4007. doi:10.1128/JCM.02056-13.
OpenUrl Abstract/FREE Full Text

[2] Buchan BW,

[3] Olson WJ,

[4] Pezewski M,

[5] Marcon MJ,

[6] Novicki T,

[7] Uphoff TS,

[8] Chandramohan L,

[9] Revell P,

[10] Ledeboer NA

[11] 2.↵
Buss SN,
Leber A,
Chapin K,
Fey PD,
Bankowski MJ,
Jones MK,
Rogatcheva M,
Kanack KJ,
Bourzac KM
. 2015. Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. J Clin Microbiol 53:915–925. doi:10.1128/JCM.02674-14.
OpenUrl Abstract/FREE Full Text

[12] Buss SN,

[13] Leber A,

[14] Chapin K,

[15] Fey PD,

[16] Bankowski MJ,

[17] Jones MK,

[18] Rogatcheva M,

[19] Kanack KJ,

[20] Bourzac KM

[21] 3.↵
DeBurger B,
Hanna S,
Powell EA,
Ventrola C,
Mortensen JE
. 2017. Utilizing BD MAX enteric bacterial panel to detect stool pathogens from rectal swabs. BMC Clin Pathol 17:7. doi:10.1186/s12907-017-0043-2.
OpenUrl CrossRef

[22] DeBurger B,

[23] Hanna S,

[24] Powell EA,

[25] Ventrola C,

[26] Mortensen JE

[27] 4.↵
Harrington SM,
Buchan BW
. 2015. Multicenter evaluation of the BD Max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes. J Clin Microbiol 53:1639–1647. doi:10.1128/JCM.03480-14.
OpenUrl Abstract/FREE Full Text

[28] Harrington SM,

[29] Buchan BW

[30] 5.↵
Huang RS,
Johnson CL,
Pritchard L,
Hepler R,
Ton TT,
Dunn JJ
. 2016. Performance of the Verigene® enteric pathogens test, Biofire FilmArray gastrointestinal panel and Luminex xTAG® gastrointestinal pathogen panel for detection of common enteric pathogens. Diagn Microbiol Infect Dis 86:336–339. doi:10.1016/j.diagmicrobio.2016.09.013.
OpenUrl CrossRef

[31] Huang RS,

[32] Johnson CL,

[33] Pritchard L,

[34] Hepler R,

[35] Ton TT,

[36] Dunn JJ

[37] 6.↵
Knabl L,
Grutsch I,
Orth-Holler D
. 2016. Comparison of the BD MAX® Enteric Bacterial Panel assay with conventional diagnostic procedures in diarrheal stool samples. Eur J Clin Microbiol Infect Dis 35:131–136. doi:10.1007/s10096-015-2517-4.
OpenUrl CrossRef PubMed

[38] Knabl L,

[39] Grutsch I,

[40] Orth-Holler D

[41] 7.↵
Madison-Antenucci S,
Relich RF,
Doyle L,
Espina N,
Fuller D,
Karchmer T,
Lainesse A,
Mortensen JE,
Pancholi P,
Veros W,
Harrington SM
. 2016. Multicenter evaluation of BD Max enteric parasite real-time PCR assay for detection of Giardia duodenalis, Cryptosporidium hominis, Cryptosporidium parvum, and Entamoeba histolytica. J Clin Microbiol 54:2681–2688. doi:10.1128/JCM.00765-16.
OpenUrl Abstract/FREE Full Text

[42] Madison-Antenucci S,

[43] Relich RF,

[44] Doyle L,

[45] Espina N,

[46] Fuller D,

[47] Karchmer T,

[48] Lainesse A,

[49] Mortensen JE,

[50] Pancholi P,

[51] Veros W,

[52] Harrington SM

[53] 8.↵
Molling P,
Nilsson P,
Ennefors T,
Ogren J,
Floren K,
Thulin Hedberg S,
Sundqvist M
. 2016. Evaluation of the BD Max enteric parasite panel for clinical diagnostics. J Clin Microbiol 54:443–444. doi:10.1128/JCM.02100-15.
OpenUrl Abstract/FREE Full Text

[54] Molling P,

[55] Nilsson P,

[56] Ennefors T,

[57] Ogren J,

[58] Floren K,

[59] Thulin Hedberg S,

[60] Sundqvist M

[61] 9.↵
Binnicker MJ
. 2015. Multiplex molecular panels for diagnosis of gastrointestinal infection: performance, result interpretation, and cost-effectiveness. J Clin Microbiol 53:3723–3728. doi:10.1128/JCM.02103-15.
OpenUrl Abstract/FREE Full Text

[62] Binnicker MJ

[63] 10.↵
Hanson KE,
Couturier MR
. 2016. Multiplexed molecular diagnostics for respiratory, gastrointestinal, and central nervous system infections. Clin Infect Dis 63:1361–1367. doi:10.1093/cid/ciw494.
OpenUrl CrossRef PubMed

[64] Hanson KE,

[65] Couturier MR

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The Utility of Multiplex Molecular Tests for Enteric Pathogens: a Micro-Comic Strip

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