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Mycobacteriology and Aerobic Actinomycetes

Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species

Barbara A. Body, Melodie A. Beard, E. Susan Slechta, Kimberly E. Hanson, Adam P. Barker, N. Esther Babady, Tracy McMillen, Yi-Wei Tang, Barbara A. Brown-Elliott, Elena Iakhiaeva, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Richard J. Wallace Jr., S. Turner, L. Curtis, Susan Butler-Wu, Jenna Rychert
Betty A. Forbes, Editor
Barbara A. Body
aDepartment of Esoteric Microbiology, Lab. Corp. of America Holdings, Burlington, North Carolina, USA
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Melodie A. Beard
aDepartment of Esoteric Microbiology, Lab. Corp. of America Holdings, Burlington, North Carolina, USA
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E. Susan Slechta
bARUP Laboratories, Salt Lake City, Utah, USA
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Kimberly E. Hanson
bARUP Laboratories, Salt Lake City, Utah, USA
cUniversity of Utah, Salt Lake City, Utah, USA
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Adam P. Barker
bARUP Laboratories, Salt Lake City, Utah, USA
cUniversity of Utah, Salt Lake City, Utah, USA
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N. Esther Babady
dMemorial Sloan Kettering Cancer Center, New York, New York, USA
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Tracy McMillen
dMemorial Sloan Kettering Cancer Center, New York, New York, USA
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Yi-Wei Tang
dMemorial Sloan Kettering Cancer Center, New York, New York, USA
gWeill Medical College of Cornell University, New York, New York, USA
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Barbara A. Brown-Elliott
eUniversity of Texas Health Science Center, Tyler, Texas, USA
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Elena Iakhiaeva
eUniversity of Texas Health Science Center, Tyler, Texas, USA
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Ravikiran Vasireddy
eUniversity of Texas Health Science Center, Tyler, Texas, USA
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Sruthi Vasireddy
eUniversity of Texas Health Science Center, Tyler, Texas, USA
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Terry Smith
eUniversity of Texas Health Science Center, Tyler, Texas, USA
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Richard J. Wallace Jr.
eUniversity of Texas Health Science Center, Tyler, Texas, USA
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S. Turner
fUniversity of Washington Medical Center, Seattle, Washington, USA
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L. Curtis
fUniversity of Washington Medical Center, Seattle, Washington, USA
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Susan Butler-Wu
fUniversity of Washington Medical Center, Seattle, Washington, USA
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Jenna Rychert
bARUP Laboratories, Salt Lake City, Utah, USA
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Betty A. Forbes
Virginia Commonwealth University Medical Center
Roles: Editor
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DOI: 10.1128/JCM.00237-18
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ABSTRACT

This multicenter study was designed to assess the accuracy and reproducibility of the Vitek MS v3.0 matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry system for identification of Mycobacterium and Nocardia species compared to DNA sequencing. A total of 963 clinical isolates representing 51 taxa were evaluated. In all, 663 isolates were correctly identified to the species level (69%), with another 231 (24%) correctly identified to the complex or group level. Fifty-five isolates (6%) could not be identified despite repeat testing. All of the tuberculous mycobacteria (45/45; 100%) and most of the nontuberculous mycobacteria (569/606; 94%) were correctly identified at least to the group or complex level. However, not all species or subspecies within the M. tuberculosis, M. abscessus, and M. avium complexes and within the M. fortuitum and M. mucogenicum groups could be differentiated. Among the 312 Nocardia isolates tested, 236 (76%) were correctly identified to the species level, with an additional 44 (14%) correctly identified to the complex level. Species within the N. nova and N. transvalensis complexes could not always be differentiated. Eleven percent of the isolates (103/963) underwent repeat testing in order to get a final result. Identification of a representative set of Mycobacterium and Nocardia species was highly reproducible, with 297 of 300 (99%) replicates correctly identified using multiple kit lots, instruments, analysts, and sites. These findings demonstrate that the system is robust and has utility for the routine identification of mycobacteria and Nocardia in clinical practice.

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Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species
Barbara A. Body, Melodie A. Beard, E. Susan Slechta, Kimberly E. Hanson, Adam P. Barker, N. Esther Babady, Tracy McMillen, Yi-Wei Tang, Barbara A. Brown-Elliott, Elena Iakhiaeva, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Richard J. Wallace Jr., S. Turner, L. Curtis, Susan Butler-Wu, Jenna Rychert
Journal of Clinical Microbiology May 2018, 56 (6) e00237-18; DOI: 10.1128/JCM.00237-18

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Evaluation of the Vitek MS v3.0 Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Mycobacterium and Nocardia Species
Barbara A. Body, Melodie A. Beard, E. Susan Slechta, Kimberly E. Hanson, Adam P. Barker, N. Esther Babady, Tracy McMillen, Yi-Wei Tang, Barbara A. Brown-Elliott, Elena Iakhiaeva, Ravikiran Vasireddy, Sruthi Vasireddy, Terry Smith, Richard J. Wallace Jr., S. Turner, L. Curtis, Susan Butler-Wu, Jenna Rychert
Journal of Clinical Microbiology May 2018, 56 (6) e00237-18; DOI: 10.1128/JCM.00237-18
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    • ABSTRACT
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KEYWORDS

MALDI-TOF
mycobacterium
Mycobacterium tuberculosis
Nocardia
mass spectrometry
mycobacteria

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