Skip to main content
  • ASM
    • Antimicrobial Agents and Chemotheraphy
    • Applied and Environmental Mircobiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems
  • Log in
  • My alerts
  • My Cart

Main menu

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
  • ASM
    • Antimicrobial Agents and Chemotheraphy
    • Applied and Environmental Mircobiology
    • Clinical Microbiology Reviews
    • Clinical and Vaccine Immunology
    • EcoSal Plus
    • Eukaryotic Cell
    • Infection and Immunity
    • Journal of Bacteriology
    • Journal of Clinical Microbiology
    • Journal of Microbiology & Biology Education
    • Journal of Virology
    • mBio
    • Microbiology and Molecular Biology Reviews
    • Microbiology Resource Announcements
    • Microbiology Spectrum
    • Molecular and Cellular Biology
    • mSphere
    • mSystems

User menu

  • Log in
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Clinical Microbiology
publisher-logosite-logo

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Accepted Manuscripts
    • Archive
    • Minireviews
  • For Authors
    • Submit a Manuscript
    • Scope
    • Editorial Policy
    • Submission, Review, & Publication Processes
    • Organization and Format
    • Errata, Author Corrections, Retractions
    • Illustrations and Tables
    • Nomenclature
    • Abbreviations and Conventions
    • Publication Fees
    • Ethics Resources and Policies
  • About the Journal
    • About JCM
    • Editor in Chief
    • Editorial Board
    • For Reviewers
    • For the Media
    • For Librarians
    • For Advertisers
    • Alerts
    • RSS
    • FAQ
  • Subscribe
    • Members
    • Institutions
Mycobacteriology and Aerobic Actinomycetes

Added Value of Xpert MTB/RIF Ultra for Diagnosis of Pulmonary Tuberculosis in a Low-Prevalence Setting

Onya Opota, Fathiah Zakham, Jesica Mazza-Stalder, Laurent Nicod, Gilbert Greub, Katia Jaton
Geoffrey A. Land, Editor
Onya Opota
Institute of Microbiology, University of Lausanne, University Hospital of Lausanne, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fathiah Zakham
Institute of Microbiology, University of Lausanne, University Hospital of Lausanne, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jesica Mazza-Stalder
Pneumology Service, University Hospital of Lausanne, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laurent Nicod
Pneumology Service, University Hospital of Lausanne, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gilbert Greub
Institute of Microbiology, University of Lausanne, University Hospital of Lausanne, Lausanne, SwitzerlandInfectious Diseases Service, University Hospital of Lausanne, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katia Jaton
Institute of Microbiology, University of Lausanne, University Hospital of Lausanne, Lausanne, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Geoffrey A. Land
Carter BloodCare & Baylor University Medical Center
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1128/JCM.01717-18
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Tables

  • Additional Files
  • TABLE 1

    Comparative performance of smear microscopy, Xpert MTB/RIF, and Xpert Ultra using culture as the gold standard (n = 196 specimens)

    TestM. tuberculosis detection
    % sensitivity (95% CI)% specificity (95% CI)
    All culture-positive specimens (n = 47)Smear-positive/culture-positive specimens (n = 23)Smear-negative/culture positive specimens (n = 24)All culture-negative specimens (n = 149)
    Smear microscopy48.94 (35.28–62.76) 23/47100 (97.49–100) 149/149
    Xpert MTB/RIF82.98 (69.86–91.11) 39/47100 (85.69–100) 23/2366.67 (46.71–82.03) 16/2497.32 (93.30–98.95) 145/149
    Xpert Ultra95.74 (85.75–99.24) 45/47100 (85.69–100) 23/2391.67 (74.15–98.52) 22/2496.64 (92.39–98.56) 144/149
  • TABLE 2

    Clinical characteristics of patients with discrepant results between M. tuberculosis culture, Xpert Ultra, and Xpert MTB/RIF and clinical characteristics of patients with Xpert Ultra positive “trace” resultsa

    PatientSex, age (yr)SpecimenSmear resultXpert MTB/RIFrpoB mutation XpertXpert Ultra resultrpoB mutation UltraMTBC culture resultRIF resistance culture resultClinical and radiologic finding
    1M, 52Bronchial aspirateNegativeNegativeNAPositive very lowNDPositive (15 days)NegativeCough, lung infiltrate, lymphadenopathy
    2F, 41SputumNegativeNegativeNAPositive very lowNDPositive (16 days)NegativeCough, wt loss, hemoptysis, fever, miliary lung infiltrate, cavitations, lymphadenopathy
    3F, 43SputumNegativeNegativeNAPositive very lowNDPositive (13 days)NegativeHIV infection, cough, hemoptysis, fever, cavitation
    4M, 17Bronchial aspirateNegativeNegativeNAPositive traceIPositive (20 days)PositiveCough, lymphadenopathy
    5F, 23Bronchial aspirateNegativeNegativeNAPositive traceIPositive (20 days)NegativeMediastinal tuberculous lymphadenitis
    6F, 46SputumNegativeNegativeNAPositive very lowNDPositive (13 days)NegativeHemoptysis, lung infiltrate
    7M, 15SputumNegativeNegativeNANegativeNAPositive (15 days)NegativeCough, hemoptysis, cavitation, fever
    8F, 26SputumNegativeNegativeNANegativeNAPositive (15 days)NegativePleural effusion
    9M, 23Bronchial aspirateNegativePositive lowNDPositive very lowNDNegativeNACough, cavitation
    10M, 62Bronchial aspirateNegativePositive very lowNDPositive mediumNDNegativeNALung infiltrate with cavitation, weight loss; history of TB 20 years before with a relapse 10 years before
    11F, 34Induced sputumNegativePositive very lowNDPositive very lowNDNegativeNANo symptoms; cavitation, M. tuberculosis culture positive on another respiratory specimen
    12M, 39Bronchoalveolar lavageNegativePositive very lowNDPositive very lowNDNegativeNAHIV infection; no symptom; new cavitation and pulmonary nodules; history of TB 7 years before
    13F, 25Bronchial aspirateNegativeNegativeNAPositive traceINegativeNACough, wt loss, asthenia, fever, lymphadenopathy, M. tuberculosis PCR and culture positive in a mediastinal cytoponction (EBUS)
    14F, 91Induced sputumNegativePositive very lowNDPositive traceIPositive (12 days)NegativeMiliary lung infiltrate
    15F, 41Bronchial aspirateNegativePositive very lowNDPositive traceIPositive (18 days)NegativeHistory of tuberculosis, new lung infiltrate
    • ↵a ND, not detected; I, indeterminate; NA, not applicable.

Additional Files

  • Tables
  • Supplemental material

    • Supplemental file 1 -

      Tables S1 (Correlation between Xpert MTB/RIF, Xpert Ultra, and culture), S2 (Correlation between Xpert MTB/RIF, Xpert Ultra semiquantitative result, and smear microscopy results), S3 (Rifampin resistance detection), and S4 (Impact of defrosting)

      PDF, 71K

PreviousNext
Back to top
Download PDF
Citation Tools
Added Value of Xpert MTB/RIF Ultra for Diagnosis of Pulmonary Tuberculosis in a Low-Prevalence Setting
Onya Opota, Fathiah Zakham, Jesica Mazza-Stalder, Laurent Nicod, Gilbert Greub, Katia Jaton
Journal of Clinical Microbiology Jan 2019, 57 (2) e01717-18; DOI: 10.1128/JCM.01717-18

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Print

Alerts
Sign In to Email Alerts with your Email Address
Email

Thank you for sharing this Journal of Clinical Microbiology article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Added Value of Xpert MTB/RIF Ultra for Diagnosis of Pulmonary Tuberculosis in a Low-Prevalence Setting
(Your Name) has forwarded a page to you from Journal of Clinical Microbiology
(Your Name) thought you would be interested in this article in Journal of Clinical Microbiology.
Share
Added Value of Xpert MTB/RIF Ultra for Diagnosis of Pulmonary Tuberculosis in a Low-Prevalence Setting
Onya Opota, Fathiah Zakham, Jesica Mazza-Stalder, Laurent Nicod, Gilbert Greub, Katia Jaton
Journal of Clinical Microbiology Jan 2019, 57 (2) e01717-18; DOI: 10.1128/JCM.01717-18
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • ACKNOWLEDGMENTS
    • FOOTNOTES
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • PDF

KEYWORDS

Mycobacterium tuberculosis
Xpert MTB/RIF
Xpert MTB/RIF Ultra
acid-fast bacilli
airborne isolation
clinical microbiology
diagnostic microbiology
point-of-care test
smear microscopy
tuberculosis

Related Articles

Cited By...

About

  • About JCM
  • Editor in Chief
  • Board of Editors
  • Editor Conflicts of Interest
  • For Reviewers
  • For the Media
  • For Librarians
  • For Advertisers
  • Alerts
  • RSS
  • FAQ
  • Permissions
  • Journal Announcements

Authors

  • ASM Author Center
  • Submit a Manuscript
  • Article Types
  • Resources for Clinical Microbiologists
  • Ethics
  • Contact Us

Follow #JClinMicro

@ASMicrobiology

       

ASM Journals

ASM journals are the most prominent publications in the field, delivering up-to-date and authoritative coverage of both basic and clinical microbiology.

About ASM | Contact Us | Press Room

 

ASM is a member of

Scientific Society Publisher Alliance

Copyright © 2019 American Society for Microbiology | Privacy Policy | Website feedback

Print ISSN: 0095-1137; Online ISSN: 1098-660X