DOI: 10.1128/JCM.01717-18
Article Figures & Data
Tables
- TABLE 1
Comparative performance of smear microscopy, Xpert MTB/RIF, and Xpert Ultra using culture as the gold standard (n = 196 specimens)
Test M. tuberculosis detection % sensitivity (95% CI) % specificity (95% CI) All culture-positive specimens (n = 47) Smear-positive/culture-positive specimens (n = 23) Smear-negative/culture positive specimens (n = 24) All culture-negative specimens (n = 149) Smear microscopy 48.94 (35.28–62.76) 23/47 100 (97.49–100) 149/149 Xpert MTB/RIF 82.98 (69.86–91.11) 39/47 100 (85.69–100) 23/23 66.67 (46.71–82.03) 16/24 97.32 (93.30–98.95) 145/149 Xpert Ultra 95.74 (85.75–99.24) 45/47 100 (85.69–100) 23/23 91.67 (74.15–98.52) 22/24 96.64 (92.39–98.56) 144/149 - TABLE 2
Clinical characteristics of patients with discrepant results between M. tuberculosis culture, Xpert Ultra, and Xpert MTB/RIF and clinical characteristics of patients with Xpert Ultra positive “trace” resultsa
Patient Sex, age (yr) Specimen Smear result Xpert MTB/RIF rpoB mutation Xpert Xpert Ultra result rpoB mutation Ultra MTBC culture result RIF resistance culture result Clinical and radiologic finding 1 M, 52 Bronchial aspirate Negative Negative NA Positive very low ND Positive (15 days) Negative Cough, lung infiltrate, lymphadenopathy 2 F, 41 Sputum Negative Negative NA Positive very low ND Positive (16 days) Negative Cough, wt loss, hemoptysis, fever, miliary lung infiltrate, cavitations, lymphadenopathy 3 F, 43 Sputum Negative Negative NA Positive very low ND Positive (13 days) Negative HIV infection, cough, hemoptysis, fever, cavitation 4 M, 17 Bronchial aspirate Negative Negative NA Positive trace I Positive (20 days) Positive Cough, lymphadenopathy 5 F, 23 Bronchial aspirate Negative Negative NA Positive trace I Positive (20 days) Negative Mediastinal tuberculous lymphadenitis 6 F, 46 Sputum Negative Negative NA Positive very low ND Positive (13 days) Negative Hemoptysis, lung infiltrate 7 M, 15 Sputum Negative Negative NA Negative NA Positive (15 days) Negative Cough, hemoptysis, cavitation, fever 8 F, 26 Sputum Negative Negative NA Negative NA Positive (15 days) Negative Pleural effusion 9 M, 23 Bronchial aspirate Negative Positive low ND Positive very low ND Negative NA Cough, cavitation 10 M, 62 Bronchial aspirate Negative Positive very low ND Positive medium ND Negative NA Lung infiltrate with cavitation, weight loss; history of TB 20 years before with a relapse 10 years before 11 F, 34 Induced sputum Negative Positive very low ND Positive very low ND Negative NA No symptoms; cavitation, M. tuberculosis culture positive on another respiratory specimen 12 M, 39 Bronchoalveolar lavage Negative Positive very low ND Positive very low ND Negative NA HIV infection; no symptom; new cavitation and pulmonary nodules; history of TB 7 years before 13 F, 25 Bronchial aspirate Negative Negative NA Positive trace I Negative NA Cough, wt loss, asthenia, fever, lymphadenopathy, M. tuberculosis PCR and culture positive in a mediastinal cytoponction (EBUS) 14 F, 91 Induced sputum Negative Positive very low ND Positive trace I Positive (12 days) Negative Miliary lung infiltrate 15 F, 41 Bronchial aspirate Negative Positive very low ND Positive trace I Positive (18 days) Negative History of tuberculosis, new lung infiltrate ↵a ND, not detected; I, indeterminate; NA, not applicable.
Additional Files
Supplemental material
- Supplemental file 1 -
Tables S1 (Correlation between Xpert MTB/RIF, Xpert Ultra, and culture), S2 (Correlation between Xpert MTB/RIF, Xpert Ultra semiquantitative result, and smear microscopy results), S3 (Rifampin resistance detection), and S4 (Impact of defrosting)
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- Supplemental file 1 -
