Mycology

Evaluation of Serum Cryptococcal Antigen Testing Using Two Novel Semiquantitative Lateral Flow Assays in Persons with Cryptococcal Antigenemia

Caleb Skipper, Kiiza Tadeo, Emily Martyn, Elizabeth Nalintya, Radha Rajasingham, David B. Meya, Bosco Kafufu, Joshua Rhein, David R. Boulware
Kimberly E. Hanson, Editor
DOI: 10.1128/JCM.02046-19

Article Figures & Data

Figures

  • FIG 1
    FIG 1

    Example images of the CrAgSQ and CryptoPS assays. (A) Example of CrAgSQ strip with an interreader discrepancy, read as either 2+ or 3+. Photo was sent to IMMY who advised that 3+ was the correct reading, based on the line intensity of T1 being greater than that of T2. (B) CryptoPS cassette read as Positive, with clear lines at T1 and C and no line at T2.

Tables

  • TABLE 1

    Contingency tables comparing the results of the CrAgSQ and CryptoPS tests and the CrAg LFA reference standard

    TABLE 1

    • ↵a The CrAgSQ had 98% sensitivity (95% CI, 0.91 to 0.99) and 98% specificity (95% CI, 0.88 to 0.99).

    • ↵b The CryptoPS had 88% sensitivity (95% CI, 0.76 to 0.95) and 95% specificity (95% CI, 0.84 to 0.99).

  • TABLE 2

    CrAg LFA titers for each semiquantitative interpreted value

    TABLE 2

    • ↵a The IMMY CrAg LFA is the U.S. Food and Drug Administration (FDA)-approved reference assay. LFA, lateral flow assay; IQR, interquartile range.

    • ↵b No CrAgSQ 5+ readings were observed in our testing, which would occur if there was a potential prozone effect.

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KEYWORDS

Cryptococcus
CrAg positive
cryptococcal antigenemia
HIV
cryptococcal meningitis
CrAg
Cryptococcus neoformans
cryptococcal antigen
human immunodeficiency virus

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