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Journal of Clinical Microbiology
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Virology

Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens

Jasper Fuk-Woo Chan, Cyril Chik-Yan Yip, Kelvin Kai-Wang To, Tommy Hing-Cheung Tang, Sally Cheuk-Ying Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, Anthony Chin-Ki Ng, Zijiao Zou, Hoi-Wah Tsoi, Garnet Kwan-Yue Choi, Anthony Raymond Tam, Vincent Chi-Chung Cheng, Kwok-Hung Chan, Owen Tak-Yin Tsang, Kwok-Yung Yuen
Alexander J. McAdam, Editor
Jasper Fuk-Woo Chan
aState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
bDepartment of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong Province, China
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
dCarol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
eHainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, and The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Cyril Chik-Yan Yip
fDepartment of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
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Kelvin Kai-Wang To
aState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
bDepartment of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong Province, China
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
dCarol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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  • ORCID record for Kelvin Kai-Wang To
Tommy Hing-Cheung Tang
gDepartment of Medicine, Queen Elizabeth Hospital, Hong Kong, Hong Kong Special Administrative Region, China
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Sally Cheuk-Ying Wong
hDepartment of Pathology, Queen Elizabeth Hospital, Hong Kong, Hong Kong Special Administrative Region, China
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Kit-Hang Leung
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Agnes Yim-Fong Fung
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Anthony Chin-Ki Ng
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Zijiao Zou
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Hoi-Wah Tsoi
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Garnet Kwan-Yue Choi
fDepartment of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
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Anthony Raymond Tam
iDepartment of Medicine, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
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Vincent Chi-Chung Cheng
fDepartment of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
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Kwok-Hung Chan
aState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
dCarol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Owen Tak-Yin Tsang
jDepartment of Medicine and Geriatrics, Princess Margaret Hospital, Hong Kong, Hong Kong Special Administrative Region, China
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Kwok-Yung Yuen
bDepartment of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong Province, China
cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
dCarol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
eHainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, and The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
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Alexander J. McAdam
Boston Children’s Hospital
Roles: Editor
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DOI: 10.1128/JCM.00310-20
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Article Information

DOI 
https://doi.org/10.1128/JCM.00310-20
PubMed 
32132196

Published By 
American Society for Microbiology Journals
History 
  • Received February 20, 2020
  • Returned for modification February 26, 2020
  • Accepted March 3, 2020
  • Published online April 23, 2020.

Copyright & Usage 
Copyright © 2020 American Society for Microbiology. All Rights Reserved.
Editors and / or Reviewers 
  • Alexander J. McAdam, Editor, Boston Children’s Hospital

Article Versions

  • Accepted Manuscript version (March 04, 2020).
  • Accepted Manuscript version (March 30, 2020).
  • You are viewing the most recent version of this article.

Author Information

  1. Jasper Fuk-Woo Chana,b,c,d,e,
  2. Cyril Chik-Yan Yipf,
  3. Kelvin Kai-Wang Toa,b,c,d,
  4. Tommy Hing-Cheung Tangg,
  5. Sally Cheuk-Ying Wongh,
  6. Kit-Hang Leungc,
  7. Agnes Yim-Fong Fungc,
  8. Anthony Chin-Ki Ngc,
  9. Zijiao Zouc,
  10. Hoi-Wah Tsoic,
  11. Garnet Kwan-Yue Choif,
  12. Anthony Raymond Tami,
  13. Vincent Chi-Chung Chengf,
  14. Kwok-Hung Chana,c,d,
  15. Owen Tak-Yin Tsangj and
  16. Kwok-Yung Yuenb,c,d,e
  1. aState Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
  2. bDepartment of Clinical Microbiology and Infection, The University of Hong Kong-Shenzhen Hospital, Shenzhen, Guangdong Province, China
  3. cDepartment of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
  4. dCarol Yu Centre for Infection, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
  5. eHainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, and The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region, China
  6. fDepartment of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
  7. gDepartment of Medicine, Queen Elizabeth Hospital, Hong Kong, Hong Kong Special Administrative Region, China
  8. hDepartment of Pathology, Queen Elizabeth Hospital, Hong Kong, Hong Kong Special Administrative Region, China
  9. iDepartment of Medicine, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, China
  10. jDepartment of Medicine and Geriatrics, Princess Margaret Hospital, Hong Kong, Hong Kong Special Administrative Region, China
  • Address correspondence to Kwok-Yung Yuen, kyyuen{at}hku.hk.
  • Jasper Fuk-Woo Chan and Cyril Chik-Yan Yip contributed equally to this work as co-first authors. Author order was determined alphabetically.

  • Citation Chan JF-W, Yip CC-Y, To KK-W, Tang TH-C, Wong SC-Y, Leung K-H, Fung AY-F, Ng AC-K, Zou Z, Tsoi H-W, Choi GK-Y, Tam AR, Cheng VC-C, Chan K-H, Tsang OT-Y, Yuen K-Y. 2020. Improved molecular diagnosis of COVID-19 by the novel, highly sensitive and specific COVID-19-RdRp/Hel real-time reverse transcription-PCR assay validated in vitro and with clinical specimens. J Clin Microbiol 58:e00310-20. https://doi.org/10.1128/JCM.00310-20.

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Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens
Jasper Fuk-Woo Chan, Cyril Chik-Yan Yip, Kelvin Kai-Wang To, Tommy Hing-Cheung Tang, Sally Cheuk-Ying Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, Anthony Chin-Ki Ng, Zijiao Zou, Hoi-Wah Tsoi, Garnet Kwan-Yue Choi, Anthony Raymond Tam, Vincent Chi-Chung Cheng, Kwok-Hung Chan, Owen Tak-Yin Tsang, Kwok-Yung Yuen
Journal of Clinical Microbiology Apr 2020, 58 (5) e00310-20; DOI: 10.1128/JCM.00310-20

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Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens
Jasper Fuk-Woo Chan, Cyril Chik-Yan Yip, Kelvin Kai-Wang To, Tommy Hing-Cheung Tang, Sally Cheuk-Ying Wong, Kit-Hang Leung, Agnes Yim-Fong Fung, Anthony Chin-Ki Ng, Zijiao Zou, Hoi-Wah Tsoi, Garnet Kwan-Yue Choi, Anthony Raymond Tam, Vincent Chi-Chung Cheng, Kwok-Hung Chan, Owen Tak-Yin Tsang, Kwok-Yung Yuen
Journal of Clinical Microbiology Apr 2020, 58 (5) e00310-20; DOI: 10.1128/JCM.00310-20
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KEYWORDS

coronavirus
COVID-19
diagnostic
emerging
PCR
SARS
Wuhan
virus

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