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Table of Contents

August 2020; Volume 58,Issue 8

Photo Quiz

  • Free
    Photo Quiz: Sudden Death
    Special Issue Photo Quiz
    Photo Quiz: Sudden Death
    Isaac H. Solomon, Robert F. Padera
  • Free
    Answer to August 2020 Photo Quiz
    Special Issue Photo Quiz
    Answer to August 2020 Photo Quiz
    Isaac H. Solomon, Robert F. Padera

Editorials

  • Free
    What Is Needed for the Response to COVID-19? A Micro-Comic Strip
    Special Issue Editorial
    What Is Needed for the Response to COVID-19? A Micro-Comic Strip
    Alexander J. McAdam
  • Free
    Special Issue on Diagnostic Testing for Severe Acute Respiratory Syndrome Coronavirus 2 and Lessons from This Pandemic
    Special Issue Editorial
    Special Issue on Diagnostic Testing for Severe Acute Respiratory Syndrome Coronavirus 2 and Lessons from This Pandemic
    Alexander J. McAdam

The Brief Case

  • Free
    The Brief Case: “Not Positive” or “Not Sure”—COVID-19-Negative Results in a Symptomatic Patient
    Special Issue The Brief Case
    The Brief Case: “Not Positive” or “Not Sure”—COVID-19-Negative Results in a Symptomatic Patient
    Bijal A. Parikh, Thomas C. Bailey, Patrick G. Lyons, Neil W. Anderson
  • Free
    Closing the Brief Case: “Not Positive” or “Not Sure”—COVID-19-Negative Results in a Symptomatic Patient
    Special Issue The Brief Case
    Closing the Brief Case: “Not Positive” or “Not Sure”—COVID-19-Negative Results in a Symptomatic Patient
    Bijal A. Parikh, Thomas C. Bailey, Patrick G. Lyons, Neil W. Anderson

Commentaries

  • Free
    Understanding, Verifying, and Implementing Emergency Use Authorization Molecular Diagnostics for the Detection of SARS-CoV-2 RNA
    Special Issue Commentary
    Understanding, Verifying, and Implementing Emergency Use Authorization Molecular Diagnostics for the Detection of SARS-CoV-2 RNA

    The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has brought a new wave of challenges to health care, particularly in the area of rapid diagnostic test development and implementation. The diagnosis of acute coronavirus disease 2019 (COVID-19) is critically dependent on the detection of SARS-CoV-2 RNA from clinical specimens (e.g., nasopharyngeal swabs). While laboratory-developed testing for SARS-CoV-2 is an...

    Stephanie L. Mitchell, Kirsten St. George, Daniel D. Rhoads, Susan M. Butler-Wu, Vaishali Dharmarha, Peggy McNult, Melissa B. Miller
    and on behalf of the American Society for Microbiology Clinical and Public Health Microbiology Committee
  • Open Access
    The Role of Antibody Testing for SARS-CoV-2: Is There One?
    Special Issue Commentary
    The Role of Antibody Testing for SARS-CoV-2: Is There One?

    The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) brought with it rapid development of both molecular and serologic assays for identification of COVID-19 infections. While Food and Drug Administration (FDA) emergency use authorization (EUA) is required for clinical application of SARS-CoV-2 molecular tests, submission for EUA is currently a voluntary process for manufacturers of serologic assays. The absence...

    Elitza S. Theel, Patricia Slev, Sarah Wheeler, Marc Roger Couturier, Susan J. Wong, Kamran Kadkhoda
  • Free
    A Public Health Laboratory Response to the Pandemic
    Special Issue Commentary
    A Public Health Laboratory Response to the Pandemic

    An outbreak of coronavirus disease 2019 (COVID-19) caused by a novel coronavirus (severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2]) began in Wuhan, Hubei, China, in December 2019 and spread rapidly worldwide. The response by the Alberta Precision Laboratories, Public Health Laboratory (ProvLab), AB, Canada, included the development and implementation of nucleic acid detection-based assays and dynamic changes in testing...

    Kanti Pabbaraju, Anita A. Wong, Mark Douesnard, Raymond Ma, Kara Gill, Paul Dieu, Kevin Fonseca, Nathan Zelyas, Graham A. Tipples
  • Free
    My Experience with SARS-CoV-2, with a Focus on Testing
    Special Issue Commentary
    My Experience with SARS-CoV-2, with a Focus on Testing

    Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) got off to a slow start in the United States. In this commentary, I describe my experience with CoV disease 2019 (COVID-19), with a focus on being tested at the University of North Carolina—Chapel Hill Respiratory Diagnostic Center on its inaugural day.

    Peggy A. Cotter
  • Free
    The COVID-19 Diagnostic Dilemma: a Clinician's Perspective
    Special Issue Commentary
    The COVID-19 Diagnostic Dilemma: a Clinician's Perspective

    In this commentary, we provide a broad overview of how the rapidly evolving coronavirus disease 2019 (COVID-19) diagnostic landscape has impacted clinical care during the COVID-19 pandemic. We review aspects of both molecular and serologic testing and discuss the logistical challenges faced with each. We also highlight the progress that has been made in the development and implementation of these assays as well as the need for ongoing...

    Ralph Rogers, Thomas O’Brien, Jad Aridi, Curt G. Beckwith
  • Free
    The First Quarter of SARS-CoV-2 Testing: the University of Washington Medicine Experience
    Special Issue Commentary
    The First Quarter of SARS-CoV-2 Testing: the University of Washington Medicine Experience

    In early March 2020, the University of Washington Medical Center clinical virology laboratory became one of the first clinical laboratories to offer testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). When we first began test development in mid-January, neither of us believed there would be more than 2 million confirmed SARS-CoV-2 infections nationwide or that we would have performed more than 150,000 real-time PCR...

    Alexander L. Greninger, Keith R. Jerome

Minireview

  • Free
    Comparative Pathogenesis of Bovine and Porcine Respiratory Coronaviruses in the Animal Host Species and SARS-CoV-2 in Humans
    Special Issue Minireview
    Comparative Pathogenesis of Bovine and Porcine Respiratory Coronaviruses in the Animal Host Species and SARS-CoV-2 in Humans

    Discovery of bats with severe acute respiratory syndrome (SARS)-related coronaviruses (CoVs) raised the specter of potential future outbreaks of zoonotic SARS-CoV-like disease in humans, which largely went unheeded. Nevertheless, the novel SARS-CoV-2 of bat ancestral origin emerged to infect humans in Wuhan, China, in late 2019 and then became a global pandemic. Less than 5 months after its emergence, millions of people worldwide have...

    Linda J. Saif, Kwonil Jung

Bacteriology

  • Free
    Bacteremia and Blood Culture Utilization during COVID-19 Surge in New York City
    Special Issue Bacteriology
    Bacteremia and Blood Culture Utilization during COVID-19 Surge in New York City

    A surge of patients with coronavirus disease 2019 (COVID-19) presenting to New York City hospitals in March 2020 led to a sharp increase in blood culture utilization, which overwhelmed the capacity of automated blood culture instruments. We sought to evaluate the utilization and diagnostic yield of blood cultures during the COVID-19 pandemic to determine prevalence and common etiologies of bacteremia and to inform a diagnostic approach...

    Jorge Sepulveda, Lars F. Westblade, Susan Whittier, Michael J. Satlin, William G. Greendyke, Justin G. Aaron, Jason Zucker, Donald Dietz, Magdalena Sobieszczyk, Justin J. Choi, Dakai Liu, Sarah Russell, Charles Connelly, Daniel A. Green

Virology

  • Open Access
    Evaluation of the QIAstat-Dx Respiratory SARS-CoV-2 Panel, the First Rapid Multiplex PCR Commercial Assay for SARS-CoV-2 Detection
    Special Issue Virology
    Evaluation of the QIAstat-Dx Respiratory SARS-CoV-2 Panel, the First Rapid Multiplex PCR Commercial Assay for SARS-CoV-2 Detection

    In the race to contain severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), efficient detection and triage of infected patients must rely on rapid and reliable testing. In this work, we performed the first evaluation of the QIAstat-Dx respiratory SARS-CoV-2 panel (QIAstat-SARS) for SARS-CoV-2 detection. This assay is the first rapid multiplex PCR (mPCR) assay, including SARS-CoV-2 detection, and is fully compatible with a non-...

    Benoit Visseaux, Quentin Le Hingrat, Gilles Collin, Donia Bouzid, Samuel Lebourgeois, Diane Le Pluart, Laurène Deconinck, François-Xavier Lescure, Jean-Christophe Lucet, Lila Bouadma, Jean-François Timsit, Diane Descamps, Yazdan Yazdanpanah, Enrique Casalino, Nadhira Houhou-Fidouh
    and the Emergency Department Influenza Study Group
  • Open Access
    Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR
    Special Issue Virology
    Evaluation of Transport Media and Specimen Transport Conditions for the Detection of SARS-CoV-2 by Use of Real-Time Reverse Transcription-PCR

    The global coronavirus (CoV) disease 2019 (COVID-19) pandemic has resulted in a worldwide shortage of viral transport media and raised questions about specimen stability. The objective of this study was to determine the stability of severe acute respiratory syndrome CoV 2 (SARS-CoV-2) RNA in specimen transport media under various storage conditions. Transport media tested included UTM, UTM-RT, ESwab, M4, and saline (0.9% NaCl). Specimen...

    Amy A. Rogers, Russell E. Baumann, Gwynngelle A. Borillo, Ron M. Kagan, Hollis J. Batterman, Marzena M. Galdzicka, Elizabeth M. Marlowe
  • Open Access
    Comparison of Four Molecular <em>In Vitro</em> Diagnostic Assays for the Detection of SARS-CoV-2 in Nasopharyngeal Specimens
    Special Issue Virology
    Comparison of Four Molecular In Vitro Diagnostic Assays for the Detection of SARS-CoV-2 in Nasopharyngeal Specimens

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the novel human coronavirus that causes coronavirus disease 2019 (COVID-19), was first discovered in December 2019 as the cause of an outbreak of pneumonia in the city of Wuhan, Hubei province, China. The clinical presentation of COVID-19 is fairly nonspecific, and symptoms overlap those of other seasonal respiratory infections concurrently circulating in the population....

    Wei Zhen, Ryhana Manji, Elizabeth Smith, Gregory J. Berry
  • Open Access
    Clinical Evaluation of Three Sample-to-Answer Platforms for Detection of SARS-CoV-2
    Special Issue Virology
    Clinical Evaluation of Three Sample-to-Answer Platforms for Detection of SARS-CoV-2

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has now spread across the globe. As part of the worldwide response, many molecular diagnostic platforms have been granted emergency use authorization (EUA) by the Food and Drug Administration (FDA) to identify SARS-CoV-2 positive patients. Our objective was to evaluate three sample-to-...

    Wei Zhen, Elizabeth Smith, Ryhana Manji, Deborah Schron, Gregory J. Berry
  • Open Access
    Comparison of Commercially Available and Laboratory-Developed Assays for <em>In Vitro</em> Detection of SARS-CoV-2 in Clinical Laboratories
    Special Issue Virology
    Comparison of Commercially Available and Laboratory-Developed Assays for In Vitro Detection of SARS-CoV-2 in Clinical Laboratories

    Multiple laboratory-developed tests (LDTs) and commercially available assays have emerged to meet diagnostic needs related to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. To date, there is limited comparison data for these different testing platforms. We compared the analytical performance of a LDT developed in our clinical laboratory based on CDC primer sets and four commercially available, FDA emergency...

    Joshua A. Lieberman, Gregory Pepper, Samia N. Naccache, Meei-Li Huang, Keith R. Jerome, Alexander L. Greninger
  • Open Access
    Open Development and Clinical Validation of Multiple 3D-Printed Nasopharyngeal Collection Swabs: Rapid Resolution of a Critical COVID-19 Testing Bottleneck
    Special Issue Virology
    Open Development and Clinical Validation of Multiple 3D-Printed Nasopharyngeal Collection Swabs: Rapid Resolution of a Critical COVID-19 Testing Bottleneck

    The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a severe international shortage of the nasopharyngeal swabs that are required for collection of optimal specimens, creating a critical bottleneck blocking clinical laboratories’ ability to perform high-sensitivity virological testing for SARS-CoV-2. To address this crisis, we designed and executed an innovative, cooperative, rapid-response...

    Cody J. Callahan, Rose Lee, Katelyn E. Zulauf, Lauren Tamburello, Kenneth P. Smith, Joe Previtera, Annie Cheng, Alex Green, Ahmed Abdul Azim, Amanda Yano, Nancy Doraiswami, James E. Kirby, Ramy A. Arnaout
  • Free
    Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2
    Special Issue Virology
    Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as the cause of a worldwide pandemic. Many commercial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received Emergency Use Authorization from the U.S. Food and Drug Administration. However, there are limited data describing their performance, in particular the performance of high-throughput SARS-CoV-2 RT-PCR systems. We analyzed the diagnostic...

    Arryn R. Craney, Priya D. Velu, Michael J. Satlin, Kathy A. Fauntleroy, Katrina Callan, Amy Robertson, Marisa La Spina, Beryl Lei, Anqi Chen, Tricia Alston, Anna Rozman, Massimo Loda, Hanna Rennert, Melissa Cushing, Lars F. Westblade
  • Free
    Large-Scale, In-House Production of Viral Transport Media To Support SARS-CoV-2 PCR Testing in a Multihospital Health Care Network during the COVID-19 Pandemic
    Special Issue Virology
    Large-Scale, In-House Production of Viral Transport Media To Support SARS-CoV-2 PCR Testing in a Multihospital Health Care Network during the COVID-19 Pandemic

    The COVID-19 pandemic has severely disrupted worldwide supplies of viral transport media (VTM) due to widespread demand for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription-PCR (RT-PCR) testing. In response to this ongoing shortage, we began production of VTM in-house in support of diagnostic testing in our hospital network. As our diagnostic laboratory was not equipped for reagent production, we took...

    Kenneth P. Smith, Annie Cheng, Amber Chopelas, Sarah DuBois-Coyne, Ikram Mezghani, Shade Rodriguez, Mustafa Talay, James E. Kirby
  • Free
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test
    Special Issue Virology
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Test

    Nucleic acid amplification tests (NAATs) are the primary means of identifying acute infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Accurate and fast test results may permit more efficient use of protective and isolation resources and allow rapid therapeutic interventions. We evaluated the analytical and clinical performance characteristics of the Xpert Xpress SARS-CoV-2 (Xpert) test, a rapid,...

    Michael J. Loeffelholz, David Alland, Susan M. Butler-Wu, Utsav Pandey, Carlo Frederico Perno, Alice Nava, Karen C. Carroll, Heba Mostafa, Emma Davies, Ashley McEwan, Jennifer L. Rakeman, Randal C. Fowler, Jean-Michel Pawlotsky, Slim Fourati, Sukalyani Banik, Padmapriya P. Banada, Shobha Swaminathan, Soumitesh Chakravorty, Robert W. Kwiatkowski, Victor C. Chu, JoAnn Kop, Rajiv Gaur, Mandy L. Y. Sin, Duy Nguyen, Simranjit Singh, Na Zhang, David H. Persing
  • Free
    Clinical Performance of the Luminex NxTAG CoV Extended Panel for SARS-CoV-2 Detection in Nasopharyngeal Specimens from COVID-19 Patients in Hong Kong
    Special Issue Virology
    Clinical Performance of the Luminex NxTAG CoV Extended Panel for SARS-CoV-2 Detection in Nasopharyngeal Specimens from COVID-19 Patients in Hong Kong

    In December 2019, the coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first reported in the Hubei province of China and later spread all over the world. There was an urgent need of a high-throughput molecular test for screening the COVID-19 patients in the community. The Luminex NxTAG CoV extended panel is a high-throughput FDA emergency use-authorized molecular...

    Jonathan Hon-Kwan Chen, Cyril Chik-Yan Yip, Jasper Fuk-Woo Chan, Rosana Wing-Shan Poon, Kelvin Kai-Wang To, Kwok-Hung Chan, Vincent Chi-Chung Cheng, Kwok-Yung Yuen
  • Free
    Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2
    Special Issue Virology
    Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2

    We compared the ability of 2 commercial molecular amplification assays (RealTime SARS-CoV-2 on the m2000 [abbreviated ACOV; Abbott] and ID Now COVID-19 [abbreviated IDNOW; Abbott]) and a laboratory-developed test (modified CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioMérieux] and amplification on QuantStudio 6 or ABI 7500 real-time PCR system [abbreviated CDC COV]) to detect severe...

    Nicholas M. Moore, Haiying Li, Debra Schejbal, Jennifer Lindsley, Mary K. Hayden
  • Free
    Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number
    Special Issue Virology
    Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number

    The outbreak of coronavirus disease 2019 (COVID-19) has spread across the world and was characterized as a pandemic. To protect medical laboratory personnel from infection, most laboratories inactivate the virus causing COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in clinical samples before testing. However, the effect of inactivation on the detection results remains unknown. Here, we used a digital PCR assay...

    Hailong Chen, Rui Wu, Yuan Xing, Quanli Du, Zerun Xue, Yanli Xi, Yujie Yang, Yangni Deng, Yuewen Han, Kaixin Li, Yang Luan, Yalan Zhang, Xiaoguang Wei, Tongtong Yu, Hao Li, Lingxiang Zhu, Shisheng Su, Hao Lian, Linping Lu, Chianru Tan, Haichao Zheng, Baozhong Chen, Pengbo Yu, Yong Guo, Chaofeng Ma
  • Free
    Clinical Performance of SARS-CoV-2 Molecular Tests
    Special Issue Virology
    Clinical Performance of SARS-CoV-2 Molecular Tests

    Molecular testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the gold standard for diagnosis of coronavirus disease 2019 (COVID-19), but the clinical performance of these tests is still poorly understood, particularly with regard to disease course, patient-specific factors, and viral shedding. From 10 March to 1 May 2020, NewYork-Presbyterian laboratories performed 27,377 SARS-CoV-2 molecular assays from 22,338...

    Daniel A. Green, Jason Zucker, Lars F. Westblade, Susan Whittier, Hanna Rennert, Priya Velu, Arryn Craney, Melissa Cushing, Dakai Liu, Magdalena E. Sobieszczyk, Amelia K. Boehme, Jorge L. Sepulveda
  • Open Access
    Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19
    Special Issue Virology
    Culture-Based Virus Isolation To Evaluate Potential Infectivity of Clinical Specimens Tested for COVID-19

    Real-time reverse transcription-PCR (RT-PCR) is currently the most sensitive method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes coronavirus disease 2019 (COVID-19). However, the correlation between detectable viral RNA and culturable virus in clinical specimens remains unclear. Here, we performed virus culture for 60 specimens that were confirmed to be positive for SARS-CoV-2 RNA by real-time RT-...

    Chung-Guei Huang, Kuo-Ming Lee, Mei-Jen Hsiao, Shu-Li Yang, Peng-Nien Huang, Yu-Nong Gong, Tzu-Hsuan Hsieh, Po-Wei Huang, Ya-Jhu Lin, Yi-Chun Liu, Kuo-Chien Tsao, Shin-Ru Shih
  • Free
    Comparison of the Accula SARS-CoV-2 Test with a Laboratory-Developed Assay for Detection of SARS-CoV-2 RNA in Clinical Nasopharyngeal Specimens
    Special Issue Virology
    Comparison of the Accula SARS-CoV-2 Test with a Laboratory-Developed Assay for Detection of SARS-CoV-2 RNA in Clinical Nasopharyngeal Specimens

    Several point-of-care (POC) molecular tests have received emergency use authorization (EUA) from the Food and Drug Administration (FDA) for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The test performance characteristics of the Accula (Mesa Biotech) SARS-CoV-2 POC test need to be evaluated to inform its optimal use. The aim of this study was to assess the test performance of the Accula SARS-CoV-2 test...

    Catherine A. Hogan, Natasha Garamani, Andrew S. Lee, Jack K. Tung, Malaya K. Sahoo, ChunHong Huang, Bryan Stevens, James Zehnder, Benjamin A. Pinsky
  • Free
    Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution
    Special Issue Virology
    Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution

    The recent emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has posed formidable challenges for clinical laboratories seeking reliable laboratory diagnostic confirmation. The swift advance of the crisis in the United States has led to Emergency Use Authorization (EUA) facilitating the availability of molecular diagnostic assays without the more rigorous examination to which tests are normally...

    Atreyee Basu, Tatyana Zinger, Kenneth Inglima, Kar-mun Woo, Onome Atie, Lauren Yurasits, Benjamin See, Maria E. Aguero-Rosenfeld

Immunoassays

  • Open Access
    Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence in Boise, Idaho
    Special Issue Immunoassays
    Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence in Boise, Idaho

    Coronavirus disease 2019 (COVID-19), the novel respiratory illness caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is associated with severe morbidity and mortality. The rollout of diagnostic testing in the United States was slow, leading to numerous cases that were not tested for SARS-CoV-2 in February and March 2020 and necessitating the use of serological testing to determine past infections. Here, we...

    Andrew Bryan, Gregory Pepper, Mark H. Wener, Susan L. Fink, Chihiro Morishima, Anu Chaudhary, Keith R. Jerome, Patrick C. Mathias, Alexander L. Greninger
  • Free
    Evaluation of the COVID-19 IgG/IgM Rapid Test from Orient Gene Biotech
    Special Issue Immunoassays
    Evaluation of the COVID-19 IgG/IgM Rapid Test from Orient Gene Biotech

    While the coronavirus disease 2019 (COVID-19) pandemic has peaked in many countries already, the current challenge is to assess population immunity on a large scale. Many serological tests are available and require urgent independent validation. Here, we report performance characteristics of Orient Gene Biotech COVID-19 IgG/IgM Rapid Test Cassette (OG) and compare it to Abbott SARS-CoV-2 IgG immunoassay (ASIA). Patients (n = ...

    Sarah Dellière, Maud Salmona, Marine Minier, Audrey Gabassi, Alexandre Alanio, Jérôme Le Goff, Constance Delaugerre, Marie-Laure Chaix
    and on behalf of Saint-Louis CORE (COvid REsearch) group
  • Free
    Performance Characteristics of Four High-Throughput Immunoassays for Detection of IgG Antibodies against SARS-CoV-2
    Special Issue Immunoassays
    Performance Characteristics of Four High-Throughput Immunoassays for Detection of IgG Antibodies against SARS-CoV-2

    The role of serologic testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in both the clinical and public health settings, will continue to evolve as we gain increasing insight into our immune response to the virus. Here, we evaluated four high-throughput serologic tests for detection of anti-SARS-CoV-2 IgG antibodies, from Abbott Laboratories (Abbott Park, IL), Epitope Diagnostics, Inc. (San Diego, CA), Euroimmun...

    Elitza S. Theel, Julie Harring, Heather Hilgart, Dane Granger

Letters to the Editor

  • Open Access
    Validation of the Hologic Aptima Unisex and Multitest Specimen Collection Kits Used for Endocervical and Male Urethral Swab Specimens (Aptima Swabs) for Collection of Samples from SARS-CoV-2-Infected Patients
    Special Issue Letter to the Editor
    Validation of the Hologic Aptima Unisex and Multitest Specimen Collection Kits Used for Endocervical and Male Urethral Swab Specimens (Aptima Swabs) for Collection of Samples from SARS-CoV-2-Infected Patients
    E. Avaniss-Aghajani, A. Sarkissian, F. Fernando, A. Avaniss-Aghajani
  • Open Access
    Comparison of Abbott ID Now, DiaSorin Simplexa, and CDC FDA Emergency Use Authorization Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Individuals Diagnosed with COVID-19
    Special Issue Letter to the Editor
    Comparison of Abbott ID Now, DiaSorin Simplexa, and CDC FDA Emergency Use Authorization Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Individuals Diagnosed with COVID-19
    Daniel D. Rhoads, Sree S. Cherian, Katharine Roman, Lisa M. Stempak, Christine L. Schmotzer, Navid Sadri
  • Open Access
    Detection of SARS-CoV-2 by Use of the Cepheid Xpert Xpress SARS-CoV-2 and Roche cobas SARS-CoV-2 Assays
    Special Issue Letter to the Editor
    Detection of SARS-CoV-2 by Use of the Cepheid Xpert Xpress SARS-CoV-2 and Roche cobas SARS-CoV-2 Assays
    Angelica Moran, Kathleen G. Beavis, Scott M. Matushek, Carol Ciaglia, Nina Francois, Vera Tesic, Nedra Love
  • Open Access
    Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2
    Special Issue Letter to the Editor
    Saliva as a Noninvasive Specimen for Detection of SARS-CoV-2
    Eloise Williams, Katherine Bond, Bowen Zhang, Mark Putland, Deborah A. Williamson
  • Open Access
    Comparison of Abbott ID Now and Abbott m2000 Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Symptomatic Patients
    Special Issue Letter to the Editor
    Comparison of Abbott ID Now and Abbott m2000 Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Symptomatic Patients
    Amanda Harrington, Brian Cox, Jennifer Snowdon, Jonathan Bakst, Erin Ley, Patricia Grajales, Jack Maggiore, Stephen Kahn
  • Free
    Evaluation of a Rapid Diagnostic Assay for Detection of SARS-CoV-2 Antigen in Nasopharyngeal Swabs
    Special Issue Letter to the Editor
    Evaluation of a Rapid Diagnostic Assay for Detection of SARS-CoV-2 Antigen in Nasopharyngeal Swabs
    Sidonie Lambert-Niclot, Alexis Cuffel, Samuel Le Pape, Christelle Vauloup-Fellous, Laurence Morand-Joubert, Anne-Marie Roque-Afonso, Jérôme Le Goff, Constance Delaugerre
    and on behalf of the AP-HP/Universities/INSERMCOVID-19 Research Collaboration
  • Free
    Patients with Common Cold Coronaviruses Tested Negative for IgG Antibody to SARS-CoV-2
    Special Issue Letter to the Editor
    Patients with Common Cold Coronaviruses Tested Negative for IgG Antibody to SARS-CoV-2
    Stephen M. Brecher, Jonathan Dryjowicz-Burek, Hongbo Yu, Sheldon Campbell, Nora Ratcliffe, Kalpana Gupta
  • Free
    Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection
    Special Issue Letter to the Editor
    Stability of SARS-CoV-2 in Phosphate-Buffered Saline for Molecular Detection
    Garrett A. Perchetti, Meei-Li Huang, Vikas Peddu, Keith R. Jerome, Alexander L. Greninger
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    Saliva as an Alternate Specimen Source for Detection of SARS-CoV-2 in Symptomatic Patients Using Cepheid Xpert Xpress SARS-CoV-2
    Special Issue Letter to the Editor
    Saliva as an Alternate Specimen Source for Detection of SARS-CoV-2 in Symptomatic Patients Using Cepheid Xpert Xpress SARS-CoV-2
    Clare McCormick-Baw, Kristi Morgan, Donna Gaffney, Yareli Cazares, Karen Jaworski, Adrienne Byrd, Kyle Molberg, Dominick Cavuoti
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    Test Agreement between Roche Cobas 6800 and Cepheid GeneXpert Xpress SARS-CoV-2 Assays at High Cycle Threshold Ranges
    Special Issue Letter to the Editor
    Test Agreement between Roche Cobas 6800 and Cepheid GeneXpert Xpress SARS-CoV-2 Assays at High Cycle Threshold Ranges
    Kari Broder, Ahmed Babiker, Charles Myers, Terri White, Heather Jones, John Cardella, Eileen M. Burd, Charles E. Hill, Colleen S. Kraft
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    Preprocedural Surveillance Testing for SARS-CoV-2 in an Asymptomatic Population in the Seattle Region Shows Low Rates of Positivity
    Special Issue Letter to the Editor
    Preprocedural Surveillance Testing for SARS-CoV-2 in an Asymptomatic Population in the Seattle Region Shows Low Rates of Positivity
    James A. Mays, Alexander L. Greninger, Keith R. Jerome, John B. Lynch, Patrick C. Mathias
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    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Assay for the Detection of SARS-CoV-2 in Oropharyngeal Swab Specimens
    Special Issue Letter to the Editor
    Multicenter Evaluation of the Cepheid Xpert Xpress SARS-CoV-2 Assay for the Detection of SARS-CoV-2 in Oropharyngeal Swab Specimens
    Hongyan Hou, Jun Chen, Yueqin Wang, Yanjun Lu, Yaowu Zhu, Bo Zhang, Feng Wang, Lie Mao, Yi-Wei Tang, Bicheng Hu, Yi Ren, Ziyong Sun

Erratum

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    Erratum for McTaggart et al., “Phylogeny and Identification of <em>Nocardia</em> Species on the Basis of Multilocus Sequence Analysis”
    Erratum
    Erratum for McTaggart et al., “Phylogeny and Identification of Nocardia Species on the Basis of Multilocus Sequence Analysis”
    L. R. McTaggart, S. E. Richardson, M. Witkowska, S. X. Zhang

Author Correction

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    Correction for Khan et al., “Emergence of a Novel Coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2: Biology and Therapeutic Options”
    Author Correction
    Correction for Khan et al., “Emergence of a Novel Coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2: Biology and Therapeutic Options”
    Suliman Khan, Rabeea Siddique, Muhammad Adnan Shereen, Ashaq Ali, Jianbo Liu, Qian Bai, Nadia Bashir, Mengzhou Xue

Masthead

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    Editorial Board
    Masthead
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Journal of Clinical Microbiology: 58 (8)

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volume 58, issue 8
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  • Laboratory Diagnosis of COVID-19: Current Issues and Challenges
  • When To Retest: an Examination of Repeat COVID-19 PCR Patterns in an Ambulatory Population
  • Performance of Abbott ID Now COVID-19 Rapid Nucleic Acid Amplification Test Using Nasopharyngeal Swabs Transported in Viral Transport Media and Dry Nasal Swabs in a New York City Academic Institution
  • Performance Characteristics of the Abbott Architect SARS-CoV-2 IgG Assay and Seroprevalence in Boise, Idaho
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